Alteration of Serum MLKL Levels and Their Association with Severity and Clinical Outcomes in Human Severe Traumatic Brain Injury: A Prospective Cohort Study DOI Creative Commons

Yidong Jin,

Han Zhang,

Min Zhou

и другие.

International Journal of General Medicine, Год журнала: 2024, Номер Volume 17, С. 5069 - 5084

Опубликована: Ноя. 1, 2024

Background: Mixed lineage kinase domain-like protein (MLKL), which modulates necroptosis, has been implicated in pathophysiological processes following acute brain injury. Here, serum MLKL was quantified to determine its prognostic significance severe traumatic injury (sTBI). Methods: This prospective cohort study enrolled 155 patients with sTBI and healthy volunteers. The severity metrics included the Glasgow Coma Scale (GCS) score Rotterdam computed tomography (CT) classification. extended outcome scale (GOSE) at posttraumatic 180 days considered as a parameter, of 1– 4 indicating poor prognosis. Univariate subsequent multivariate analyses were used for independent factorial investigation. Results: Compared controls, displayed profoundly elevated levels. In framework restricted cubic spline analysis, levels linearly correlated likelihood mortality, overall survival, Serum not only independently GCS, CT scores GOSE scores, but also predictive death, Subgroup analysis showed that exhibited negligible interactions age, sex, hypertension, diabetes, smoking habits, alcohol consumption distinguish possibility Within context receiver operating characteristic curve had strong discrimination effectiveness death prognosis and, contrast GCS have equivalent ability. Conclusion: Extreme elevation is intimately related trauma severity, neurological outcomes, suggesting may act potential predictor facilitating stratification prediction sTBI. Keywords: injury, mixed protein, outcome, biomarkers

Язык: Английский

Neurovascular unit, neuroinflammation and neurodegeneration markers in brain disorders DOI Creative Commons
Duraisamy Kempuraj,

Kirk D. Dourvetakis,

Jessica R. Cohen

и другие.

Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18

Опубликована: Окт. 25, 2024

Neurovascular unit (NVU) inflammation via activation of glial cells and neuronal damage plays a critical role in neurodegenerative diseases. Though the exact mechanism disease pathogenesis is not understood, certain biomarkers provide valuable insight into pathogenesis, severity, progression therapeutic efficacy. These markers can be used to assess pathophysiological status brain including neurons, astrocytes, microglia, oligodendrocytes, specialized microvascular endothelial cells, pericytes, NVU, blood-brain barrier (BBB) disruption. Damage or derangements tight junction (TJ), adherens (AdJ), gap (GJ) components BBB lead increased permeability neuroinflammation various disorders disorders. Thus, neuroinflammatory evaluated blood, cerebrospinal fluid (CSF), tissues determine neurological progression, responsiveness. Chronic common age-related Alzheimer's (AD), Parkinson's (PD), dementia. Neurotrauma/traumatic injury (TBI) also leads acute chronic responses. The expression some may altered many years even decades before onset In this review, we discuss neuroinflammation, neurodegeneration associated with disorders, especially those neurovascular pathologies. CSF, tissues. Neurofilament light (NfL), ubiquitin C-terminal hydrolase-L1 (UCHL1), fibrillary acidic protein (GFAP), Ionized calcium-binding adaptor molecule 1 (Iba-1), transmembrane 119 (TMEM119), aquaporin, endothelin-1, platelet-derived growth factor receptor beta (PDGFRβ) are important markers. Recent BBB-on-a-chip modeling offers promising potential for providing an in-depth understanding neurotherapeutics. Integration these clinical practice could potentially enhance early diagnosis, monitor improve outcomes.

Язык: Английский

Процитировано

5

The blood-brain barrier as a treatment target for neurodegenerative disorders DOI
Ruslan Rust, Abhay P. Sagare, Mingzi Zhang

и другие.

Expert Opinion on Drug Delivery, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Introduction The blood-brain barrier (BBB) is a vascular endothelial membrane which restricts entry of toxins, cells and microorganisms into the brain. At same time, BBB supplies brain with nutrients, key substrates for DNA RNA synthesis, regulatory molecules, removes metabolic waste products from to blood. breakdown and/or dysfunction have been shown in neurogenerative disorders including Alzheimer's disease (AD). Current data suggests that these changes may initiate contribute neuronal, synaptic cognitive dysfunction, possibly other aspects neurodegenerative processes.

Язык: Английский

Процитировано

0

Engineered nanoparticles for the treatment of Alzheimer’s disease DOI Creative Commons
Jia Jia, Shuang Zhao, Jinghan Zhao

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Апрель 3, 2025

Alzheimer’s disease (AD) is one of the most common diseases characterized by neurodegeneration and becoming a major public health problem worldwide. AD manifested mainly progressive impairments in cognition, emotion, language memory elderly population. Many treatment strategies have been explored for decades; however, there still no effective way to address root cause pathogenesis, only target symptoms improve patient cognitive outcomes. Intracerebral administration difficult because challenges posed blood‒brain barrier (BBB). NPs are materials with sizes between 1 100 nm that can biocompatibility, extend half-life, transport macromolecules, be delivered across BBB central nervous system, exhibit good targeting capabilities. provide new ideas terms their antiaging, antineuroinflammatory, antioxidative, nerve repair-promoting effects. In this manuscript, we first describe relationship BBB. Second, introduce application nanoparticles treatment. Finally, summarize faced AD.

Язык: Английский

Процитировано

0

Evidence Suggesting Prolonged Neuroinflammation in a Subset of Children after Moderate/Severe TBI: A UCLA RAPBI Study DOI Creative Commons

Courtney McCabe,

Emily L. Dennis, Hannah M. Lindsey

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Abstract Traumatic brain injury (TBI) presents a public health concern as leading cause of death and disability in children. Pediatric populations are particularly vulnerable to adverse outcomes following TBI due periods rapid growth, synaptic pruning, myelination. patients with moderate-severe (msTBI) healthy controls were evaluated from the post-acute (2-5 months) chronic phase (13-19 recovery using diffusion magnetic resonance imaging (dMRI) interhemispheric transfer time (IHTT), which is an event-related potential measure speed information across corpus callosum. We previously identified two subgroups based on IHTT, one group showing significantly slower IHTT (TBI-slow), poorer cognitive performance, progressive structural damage. In contrast, other (TBI-normal) did not differ or performance showed relative over time. Here, we examined differences restricted (RDI), dMRI metric sensitive inflammation. Comparing TBI-slow, TBI-normal, RDI cross-sectionally, connectometry analysis revealed higher white matter TBI-slow compared both control TBI-normal groups. Longitudinal analyses indicated that while groups exhibited decrease time, suggesting resolution neuroinflammation recovery, decreases smaller. The between suggest inflammation may play key role prolonged including structure, symptom reports, pediatric msTBI.

Язык: Английский

Процитировано

0

Artificial intelligence-driven prediction and validation of blood-brain barrier permeability and absorption, distribution, metabolism, excretion profiles in Natural Product Research Laboratory compounds DOI Creative Commons
Jai‐Sing Yang,

E.P. Huang,

Ken Ying-Kai Liao

и другие.

Biomedicine, Год журнала: 2024, Номер 14(4)

Опубликована: Дек. 1, 2024

Introduction: Our previous research demonstrated that a large language model (LLM) based on the transformer architecture, specifically MegaMolBART encoder with an XGBoost classifier, effectively predicts blood-brain barrier (BBB) permeability of compounds. However, coefficients compounds can traverse this remain unclear. Additionally, absorption, distribution, metabolism, and excretion (ADME) characteristics substances obtained from Natural Product Research Laboratory (NPRL) at China Medical University Hospital (CMUH) have not yet been determined.

Язык: Английский

Процитировано

1

Bursts of brain erosion: seizures and age-dependent neurological vulnerability DOI
Noémie Cresto, Laurent Givalois, Jérôme Badaut

и другие.

Trends in Molecular Medicine, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

1

Evaluation of Blood–Brain Barrier Disruption Using Low- and High-Molecular-Weight Complexes in a Single Brain Sample in a Rat Traumatic Brain Injury Model: Comparison to an Established Magnetic Resonance Imaging Technique DOI Open Access

Vladislav Zvenigorodsky,

Benjamin F. Gruenbaum, Ilan Shelef

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11241 - 11241

Опубликована: Окт. 19, 2024

Traumatic brain injury (TBI), a major cause of death and disability among young people, leads to significant public health economic challenges. Despite its frequency, treatment options remain largely unsuitable. However, examination the blood–brain barrier (BBB) can assist with understanding mechanisms dynamics dysfunction, which affects TBI sufferers secondarily injury. Here, we present rat model focused on two standard BBB assessment markers, high- low-molecular-weight complexes, in order understand disruption. In addition, tested new technique evaluate disruption single set, comparing neuroimaging. A total 100 Sprague–Dawley rats were separated into following five groups: naive (n = 20 rats), control administration 60 rats). Rats assessed at different time points after measure using low- high-molecular-weight complexes. Neurological severity score was evaluated baseline 24 h TBI. During neurological exam TBI, scanned magnetic resonance imaging euthanized for permeability. We found that markers displayed examples same set tissues over period week. Our innovative protocol assessing permeability complexes showed appropriate results. Additionally, determined lower limit sensitivity, therefore demonstrating accuracy this method.

Язык: Английский

Процитировано

0

Editorial: 15 years of frontiers in cellular neuroscience: blood brain barrier modulation and dysfunction in brain diseases DOI Creative Commons
Duraisamy Kempuraj, Stefania Ceruti

Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18

Опубликована: Окт. 29, 2024

The blood-brain barrier (BBB) is a dynamic structure that regulates transport (influx and efflux) of molecules between brain parenchyma blood circulation protects the from various pathogens, neurotoxic periphery (Zapata-Acevedo et al., 2024) drugs (Ronaldson Davis, 2024). These protective actions are achieved due to presence tight junctions (TJ) with claudin 5, occluden, junctional adhesion (JAMs), zonula occludens-1 (ZO-1), adherens (AJ) Ve-Cadherin, catenin, gap (GJ) connexins adjacent endothelial cells provide physical peripheral (Kempuraj 2020;Andjelkovic 2023;Haruwaka 2019). Microvascular in express many transporters regulate molecules, including glucose. Neurovascular unit (NVU) consists microvascular cells, astrocytes, pericytes (overall forming BBB) neurons. An intact BBB maintains homeostasis microenvironment for normal neuronal signaling, while disrupted BBB, derangement junction proteins associated increased permeability observed neuroinflammatory neurodegenerative disorders (Sweeney 2018;Kempuraj 2020). Neuroinflammation plays an important role such as chronic diseases Alzheimer's disease, Parkinson's Multiple Sclerosis (MS), stroke, traumatic injury (TBI) Systemic inflammatory can cause exacerbate responses (Paouri Georgopoulos, 2019;Cheng 2022). This research topic "Blood-brain modulation dysfunction diseases" presents collection following articles demonstrate disruption pathogenesis how it be therapeutically overcome.Gemfibrozil lipid-lowering drug showed neuroprotective effects by inhibiting glial inflammation, oxidative stress apoptosis (Ivraghi A previous study has shown gemfibrozil's therapeutic beneficial experimental autoimmune encephalomyelitis (EAE), animal model demyelinating disease MS (Dasgupta 2007). Gemfibrozil known suppress expression through its ligand peroxisome proliferator-activated receptor alpha (PPARα). paper Mondal al. this special shows gemfibrozil integrity BBB/blood-spinal cord (BSB), enhances Tregs, decreases EAE PPARβ, but not PPARα mice (Mondal Further, above indicates immunomodulatory PPARβ may explored intervention 2024).Pericytes flow thus contribute pathophysiology NVU (Bhowmick Pericytes highly plastic contractile microvessels, maintenance integrity, angiogenesis, control leukocyte transmigration (Alarcon-Martinez 2021). In their review article topic, Fu highlight pericyte biomarkers, stem cell paracrine functions, neuroinflammation especially ischemic stroke (Fu 2023). these authors suggest could targets disorders, TBI provides significant current information on fundamental still partially unknown contributors NVU/BBB functions 2023).Oxidative causes damage TBI. Inhibition histone deacetylases (HDACs) been decrease level reducing reactive oxygen species (Lu paper, Inoue studied mechanism HDAC inhibition using vascular (Inoue They report incubation immortalized human (HCMEC/D3) treated W2A-16 (an inhibitor) retained property trans-endothelial electrical resistance (TEER) levels compared untreated cells. study, were cultured hydrogen peroxidase then medium was switched or without cultured. concludes formation 2024).Microglia brain-resident immune present M0 (resting), M1 (proinflammatory) M2 anti-inflammatory phenotypes (Wendimu Hooks, Microglial activation implicated diseases. recent discussed microglia health (Mayer Fischer, Deng crosstalk communication brain, heart, spleen after (Deng discuss microglia-associated network inflammation induces cardiovascular disorders. they indicate involve permeability, entry into brain-derived organs monocytes 2023).Detection biomarkers cerebrospinal fluid (CSF), blood, tissues, validation vitro culture models useful diagnoses assessing severity components (Kadry Kempuraj overview most markers NVU/BBB, expressed D, derangements TJ, AJ, GJ lead resulting edema, 2024).Additionally, highlights BBB-on-a-chip modeling offers promising potential pre-clinical in-depth understanding pathologies neurotherapeutics.Neuroinflammatory show dysfunctions permeability. Understanding mechanisms underlying pathological events assessment identify innovative treatment options several insights which development more effective

Язык: Английский

Процитировано

0

Alteration of Serum MLKL Levels and Their Association with Severity and Clinical Outcomes in Human Severe Traumatic Brain Injury: A Prospective Cohort Study DOI Creative Commons

Yidong Jin,

Han Zhang,

Min Zhou

и другие.

International Journal of General Medicine, Год журнала: 2024, Номер Volume 17, С. 5069 - 5084

Опубликована: Ноя. 1, 2024

Background: Mixed lineage kinase domain-like protein (MLKL), which modulates necroptosis, has been implicated in pathophysiological processes following acute brain injury. Here, serum MLKL was quantified to determine its prognostic significance severe traumatic injury (sTBI). Methods: This prospective cohort study enrolled 155 patients with sTBI and healthy volunteers. The severity metrics included the Glasgow Coma Scale (GCS) score Rotterdam computed tomography (CT) classification. extended outcome scale (GOSE) at posttraumatic 180 days considered as a parameter, of 1– 4 indicating poor prognosis. Univariate subsequent multivariate analyses were used for independent factorial investigation. Results: Compared controls, displayed profoundly elevated levels. In framework restricted cubic spline analysis, levels linearly correlated likelihood mortality, overall survival, Serum not only independently GCS, CT scores GOSE scores, but also predictive death, Subgroup analysis showed that exhibited negligible interactions age, sex, hypertension, diabetes, smoking habits, alcohol consumption distinguish possibility Within context receiver operating characteristic curve had strong discrimination effectiveness death prognosis and, contrast GCS have equivalent ability. Conclusion: Extreme elevation is intimately related trauma severity, neurological outcomes, suggesting may act potential predictor facilitating stratification prediction sTBI. Keywords: injury, mixed protein, outcome, biomarkers

Язык: Английский

Процитировано

0