Nobiletin and Eriodictyol Suppress Release of IL-1β, CXCL8, IL-6, and MMP-9 from LPS, SARS-CoV-2 Spike Protein, and Ochratoxin A-Stimulated Human Microglia

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 636 - 636

Опубликована: Янв. 14, 2025

Neuroinflammation is involved in various neurological and neurodegenerative disorders which the activation of microglia one key factors. In this study, we examined anti-inflammatory effects flavonoids nobiletin (5,6,7,8,3′,4′-hexamethoxyflavone) eriodictyol (3′,4′,5,7-tetraxydroxyflavanone) on human cell line stimulated by either lipopolysaccharide (LPS), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) full-length Spike protein (FL-Spike), or mycotoxin ochratoxin A (OTA). Human were preincubated with (10, 50, 100 µM) for h, following which, they 24 h. The inflammatory mediators interleukin-1 beta (IL-1β), chemokine (C-X-C motif) ligand 8 (CXCL8), IL-6, matrix metalloproteinase-9 (MMP-9) quantified culture supernatant enzyme-linked immunosorbent assay (ELISA). Both significantly inhibited LPS, FL-Spike, OTA-stimulated release IL-1β, CXCL8, MMP-9 at 50 µM, while, most cases, was also effective 10 pronounced reductions µM. These findings suggest that both are potent inhibitors pathogen-stimulated microglial mediators, highlighting their potential therapeutic application neuroinflammatory diseases, such as long COVID.

Язык: Английский

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The dual role of microglia in Alzheimer’s disease: from immune regulation to pathological progression

Frontiers in Aging Neuroscience, Год журнала: 2025, Номер 17

Опубликована: Март 27, 2025

Alzheimer’s disease (AD) is a widespread neurodegenerative disorder and one of the major challenges for public health. Despite extensive research, role microglia in AD remains complex dual. The aim this review to summarize most recent advances research regarding dual concerning both immunomodulation pathological progression by considering mechanisms activation microglia, effects on Aβ clearance, tau pathology, impacts due genetic variations microglial functions. Among these findings are status M1 M2 phenotypes, crucial that variants like TREM2 have modulating response microglia. This describes how modulation signaling pathway might be exploited therapeutically treatment underlines relevance personalized medicine approach.

Язык: Английский

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Neuron-Glial2 (NG2) Cells: A Promising Target for Neuroinflammation-Related Neurodegeneration

Translational Research in Anatomy, Год журнала: 2025, Номер unknown, С. 100395 - 100395

Опубликована: Март 1, 2025

Язык: Английский

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Bridging the Gap: Endothelial Dysfunction and the Role of iPSC-Derived Endothelial Cells in Disease Modeling

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13275 - 13275

Опубликована: Дек. 11, 2024

Endothelial cells (ECs) are crucial for vascular health, regulating blood flow, nutrient exchange, and modulating immune responses inflammation. The impairment of these processes causes the endothelial dysfunction (ED) characterized by oxidative stress, inflammation, permeability, extracellular matrix remodeling. While primary ECs have been widely used to study ED in vitro, their limitations—such as short lifespan donor variability—pose challenges. In this context, induced iECs derived from pluripotent stem offer an innovative solution, providing unlimited source explore disease-specific features ED. Recent advancements 3D models microfluidic systems enhanced physiological relevance iEC-based better mimicking microenvironment. These innovations bridge gap between understanding mechanisms drug developing screening prevent or treat This review highlights current state iEC technology a model non-vascular disorders, including diabetes, cardiovascular, neurodegenerative diseases.

Язык: Английский

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Acute subdural hematomas in nonagenarians and centenarians

Journal of Clinical Neuroscience, Год журнала: 2024, Номер 132, С. 110997 - 110997

Опубликована: Дек. 26, 2024

Язык: Английский

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