Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Июль 17, 2024

Loop-mediated isothermal amplification (LAMP) is a novel method for nucleic acid detection known its properties, high efficiency, sensitivity, and specificity. LAMP employs 4 to 6 primers targeting 8 regions of the desired sequence, allowing at temperatures between 60 65°C production up 10

Язык: Английский

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Comparison of four isothermal amplification techniques: LAMP, SEA, CPA, and RPA for the identification of chicken adulteration

Food Control, Год журнала: 2024, Номер 159, С. 110302 - 110302

Опубликована: Янв. 9, 2024

Язык: Английский

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Rapid and sensitive detection of Salmonella in agro-Food and environmental samples: A review of advances in rapid tests and biosensors

Journal of Microbiological Methods, Год журнала: 2024, Номер 219, С. 106897 - 106897

Опубликована: Фев. 9, 2024

Язык: Английский

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A dual-signal amplification strategy based on rolling circle amplification and APE1-assisted amplification for highly sensitive and specific miRNA analysis for early diagnosis of alzheimer's disease

Talanta, Год журнала: 2024, Номер 272, С. 125747 - 125747

Опубликована: Фев. 10, 2024

Язык: Английский

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One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives

Trends in biotechnology, Год журнала: 2024, Номер 42(11), С. 1410 - 1426

Опубликована: Июль 20, 2024

Язык: Английский

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Multi-target microfluidic loop-mediated isothermal amplification technology for the detection of foodborne pathogens in various food matrices

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 343751 - 343751

Опубликована: Янв. 1, 2025

Язык: Английский

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Recent advancements with loop-mediated isothermal amplification (LAMP) in assessment of the species authenticity with meat and seafood products

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 22

Опубликована: Март 18, 2024

Species adulteration or mislabeling with meat and seafood products could negatively affect the fair trade, wildlife conservation, food safety, religion aspect, even public health. While PCR-based methods remain gold standard for assessment of species authenticity, there is an urgent need alternative testing platforms that are rapid, accurate, simple, portable. Owing to its ease use, low cost, rapidity, LAMP becoming increasingly used method in analysis detecting mislabeling. In this review, we outline how features have been leveraged authentication test products. Meanwhile, as trend detection rapid instrument-free, it great necessity carry out end-point visual detection, principles various colorimetry also reviewed. Moreover, aim enhance reaction, different strategies summarized either suppress nonspecific amplification, avoid results amplification. Finally, microfluidic chip a promising point-of-care method, which has subject deal research directed toward development platforms-based systems authenticity

Язык: Английский

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Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2024, Номер 16(3)

Опубликована: Май 1, 2024

Abstract MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role gene expression regulation. Salivary miRNA, particular, stands out for its non‐invasive collection method ease of accessibility, offering promising avenues the development point‐of‐care diagnostics a spectrum diseases, including cancer, neurodegenerative disorders, infectious diseases. Such promises rapid precise diagnosis, enabling timely treatment. Despite advancements salivary miRNA‐based testing, challenges persist quantification, multiplexing, sensitivity, specificity, particularly miRNA at low concentrations complex biological mixtures. This work delves into these challenges, focusing on application tests use. We explore biogenesis analyze quantitative disease relevance infection, disorders. also examined recent progress extraction, amplification, multiplexed detection methods. study offers comprehensive view testing (POCT). Its successful advancement could revolutionize early detection, monitoring, management various conditions, enhancing healthcare outcomes. article is categorized under: Diagnostic Tools > Biosensing Nanodevices

Язык: Английский

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Molecular diagnostics: past, present, and future

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 3 - 23

Опубликована: Янв. 1, 2024

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Evaluating Commercial Loop-Mediated Isothermal Amplification Master Mixes for Enhanced Detection of Foodborne Pathogens

Foods, Год журнала: 2024, Номер 13(11), С. 1635 - 1635

Опубликована: Май 24, 2024

Loop-mediated isothermal amplification, LAMP, is nowadays the most popular nucleic acid amplification technique, and as such, several commercial, ready-to-use master mixes have flourished. Unfortunately, independent studies to determine their performance are limited. The current study performed an evaluation of existing commercial LAMP WarmStart® Kit, LavaLAMP™ DNA Master Mix, Saphir Bst Turbo GreenMaster, OptiGene Fast Mix ISO-004, SynLAMP Mix. To reduce bias, three different genes, namely ttr (Salmonella spp.), rfbE (E. coli O157), hly (Listeria monocytogenes), were targeted. comparison was based on speed, with decreasing concentrations, effect five typical reaction additives (betaine, DMSO, pullulan, TMAC, GuHCl). Significant differences observed among mixes. provided fastest showed less detrimental effects associated supplements evaluated. Out chemicals tested, pullulan best results in terms speed. It noteworthy that impacted differently. Overall, provides insights into mixes, which can be value for scientific community better select appropriate reagents when developing new methods.

Язык: Английский

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