Point-of-Care Detection of Mycobacterium in Bovine Feces Using a Portable Real-time Loop-mediated Isothermal Amplification System DOI
Wenjing Sun, Caiming Li, Xiaolin Wu

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137857 - 137857

Published: April 1, 2025

Language: Английский

Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview DOI Creative Commons
Nan Yang, Han Zhang,

Xiu Li Han

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 17, 2024

Loop-mediated isothermal amplification (LAMP) is a novel method for nucleic acid detection known its properties, high efficiency, sensitivity, and specificity. LAMP employs 4 to 6 primers targeting 8 regions of the desired sequence, allowing at temperatures between 60 65°C production up 10

Language: Английский

Citations

20
Comparison of four isothermal amplification techniques: LAMP, SEA, CPA, and RPA for the identification of chicken adulteration DOI
Song Yan, Cuiling Li, Hangzhen Lan

et al.

Food Control, Journal Year: 2024, Volume and Issue: 159, P. 110302 - 110302

Published: Jan. 9, 2024

Language: Английский

Citations

16
Rapid and sensitive detection of Salmonella in agro-Food and environmental samples: A review of advances in rapid tests and biosensors DOI
Siti Nur Hazwani Oslan, Nik Yusnoraini Yusof, Si Jie Lim

et al.

Journal of Microbiological Methods, Journal Year: 2024, Volume and Issue: 219, P. 106897 - 106897

Published: Feb. 9, 2024

Language: Английский

Citations

13
A dual-signal amplification strategy based on rolling circle amplification and APE1-assisted amplification for highly sensitive and specific miRNA analysis for early diagnosis of alzheimer's disease DOI
Juan Xie, Jing Chen, Ya Zhang

et al.

Talanta, Journal Year: 2024, Volume and Issue: 272, P. 125747 - 125747

Published: Feb. 10, 2024

Language: Английский

Citations

7
Recent advancements with loop-mediated isothermal amplification (LAMP) in assessment of the species authenticity with meat and seafood products DOI
Shihui Wang,

Hongwei Song,

Tianlong Wang

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 22

Published: March 18, 2024

Species adulteration or mislabeling with meat and seafood products could negatively affect the fair trade, wildlife conservation, food safety, religion aspect, even public health. While PCR-based methods remain gold standard for assessment of species authenticity, there is an urgent need alternative testing platforms that are rapid, accurate, simple, portable. Owing to its ease use, low cost, rapidity, LAMP becoming increasingly used method in analysis detecting mislabeling. In this review, we outline how features have been leveraged authentication test products. Meanwhile, as trend detection rapid instrument-free, it great necessity carry out end-point visual detection, principles various colorimetry also reviewed. Moreover, aim enhance reaction, different strategies summarized either suppress nonspecific amplification, avoid results amplification. Finally, microfluidic chip a promising point-of-care method, which has subject deal research directed toward development platforms-based systems authenticity

Language: Английский

Citations

5
Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies DOI
Zhikun Zhang, Tianyi Liu, Ming Dong

et al.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(3)

Published: May 1, 2024

Abstract MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role gene expression regulation. Salivary miRNA, particular, stands out for its non‐invasive collection method ease of accessibility, offering promising avenues the development point‐of‐care diagnostics a spectrum diseases, including cancer, neurodegenerative disorders, infectious diseases. Such promises rapid precise diagnosis, enabling timely treatment. Despite advancements salivary miRNA‐based testing, challenges persist quantification, multiplexing, sensitivity, specificity, particularly miRNA at low concentrations complex biological mixtures. This work delves into these challenges, focusing on application tests use. We explore biogenesis analyze quantitative disease relevance infection, disorders. also examined recent progress extraction, amplification, multiplexed detection methods. study offers comprehensive view testing (POCT). Its successful advancement could revolutionize early detection, monitoring, management various conditions, enhancing healthcare outcomes. article is categorized under: Diagnostic Tools > Biosensing Nanodevices

Language: Английский

Citations

5
One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives DOI

Xingyu Ye,

Haoyang Wu, Jinghan Liu

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(11), P. 1410 - 1426

Published: July 20, 2024

Language: Английский

Citations

5
Evaluating Commercial Loop-Mediated Isothermal Amplification Master Mixes for Enhanced Detection of Foodborne Pathogens DOI Creative Commons
Ana Costa-Ribeiro, Alexandre Lamas, Alejandro Garrido‐Maestu

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(11), P. 1635 - 1635

Published: May 24, 2024

Loop-mediated isothermal amplification, LAMP, is nowadays the most popular nucleic acid amplification technique, and as such, several commercial, ready-to-use master mixes have flourished. Unfortunately, independent studies to determine their performance are limited. The current study performed an evaluation of existing commercial LAMP WarmStart® Kit, LavaLAMP™ DNA Master Mix, Saphir Bst Turbo GreenMaster, OptiGene Fast Mix ISO-004, SynLAMP Mix. To reduce bias, three different genes, namely ttr (Salmonella spp.), rfbE (E. coli O157), hly (Listeria monocytogenes), were targeted. comparison was based on speed, with decreasing concentrations, effect five typical reaction additives (betaine, DMSO, pullulan, TMAC, GuHCl). Significant differences observed among mixes. provided fastest showed less detrimental effects associated supplements evaluated. Out chemicals tested, pullulan best results in terms speed. It noteworthy that impacted differently. Overall, provides insights into mixes, which can be value for scientific community better select appropriate reagents when developing new methods.

Language: Английский

Citations

4
Molecular diagnostics: past, present, and future DOI
Mrutyunjay Suar, Namrata Misra,

Madhabi Madhusmita Bhanjadeo

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 3 - 23

Published: Jan. 1, 2024

Citations

4
Food Authentication Using the Loop-Mediated Isothermal Amplification (LAMP) Method: Current Trends and Future Prospects DOI
Jingbin Zhang, Marti Z. Hua, Huan Chen

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104874 - 104874

Published: Jan. 1, 2025

Language: Английский

Citations

0