Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration

Biomaterials Research, Год журнала: 2023, Номер 27(1)

Опубликована: Фев. 5, 2023

In recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more cells from joint cavity defect area promote regeneration.In this study, we used ECM alone as a bioink low-temperature deposition manufacturing (LDM) 3D printing then successfully fabricated hierarchical porous scaffold incorporating GDF-5.Comparative vitro experiments showed that 7% scaffolds had best biocompatibility. After addition of GDF-5 protein, significantly improved bone marrow mesenchymal cell (BMSC) migration chondrogenic differentiation. Most importantly, vivo results ECM/GDF-5 enhanced repair.In conclusion, study reports construction new based concept regeneration, believe our findings will provide treatment strategy for AC repair.

Язык: Английский

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Cartilage lacuna-biomimetic hydrogel microspheres endowed with integrated biological signal boost endogenous articular cartilage regeneration

Bioactive Materials, Год журнала: 2024, Номер 41, С. 61 - 82

Опубликована: Июль 12, 2024

Despite numerous studies on chondrogenesis, the repair of cartilage-particularly reconstruction cartilage lacunae through an all-in-one advanced drug delivery system remains limited. In this study, we developed a lacuna-like hydrogel microsphere endowed with integrated biological signals, enabling sequential immunomodulation and endogenous articular regeneration. We first chondrogenic growth factor transforming factor-β3 (TGF-β3) into mesoporous silica nanoparticles (MSNs). Then, TGF-β3@MSNs insulin-like 1 (IGF-1) were encapsulated within microspheres made polydopamine (pDA). final step, factor-loaded MSN@pDA chitosan (CS) containing platelet-derived factor-BB (PDGF-BB) blended to produce factors loaded composite (GFs@μS) using microfluidic technology. The presence pDA reduced initial acute inflammatory response, early, robust release PDGF-BB aided in attracting stem cells. Over subsequent weeks, continuous IGF-1 TGF-β3 amplified chondrogenesis matrix formation. μS incorporated acellular extracellular (ACECM) combined polydopamine-modified polycaprolactone (PCL) structure tissue-engineered scaffold that mimicked evenly distributed matrix, resulting enhanced patellar protection. This research provides strategic pathway for optimizing ensuring prolonged microenvironmental remodeling, leading efficient

Язык: Английский

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Stem cell recruitment polypeptide hydrogel microcarriers with exosome delivery for osteoarthritis treatment

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 27, 2024

With the accelerated aging tendency, osteoarthritis (OA) has become an intractable global public health challenge. Stem cells and their derivative exosome (Exo) have shown great potential in OA treatment. Research this area tends to develop functional microcarriers for stem cell Exo delivery improve therapeutic effect. Herein, we a novel system of Exo-encapsulated cell-recruitment hydrogel from liquid nitrogen-assisted microfluidic electrospray Benefited advanced droplet generation capability microfluidics mild cryogelation procedure, resultant particles show uniform size dispersion excellent biocompatibility. Moreover, acryloylated recruitment peptides SKPPGTSS are directly crosslinked within by ultraviolet irradiation, thus simplifying peptide coupling process preventing its premature release. The SKPPGTSS-modified can recruit endogenous promote cartilage repair released further enhances performance through synergistic effects. These features suggest that proposed microcarrier is promising candidate

Язык: Английский

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Rapid Customization of Biomimetic Cartilage Scaffold with Stem Cell Capturing and Homing Capabilities for In Situ Inductive Regeneration of Osteochondral Defects

Advanced Functional Materials, Год журнала: 2024, Номер 34(28)

Опубликована: Май 31, 2024

Abstract 3D printing of articular cartilage tissue faces challenges like replicating its complex structure, time‐consuming in vitro stem cell culture, and a lack robust situ regeneration methods for osteochondral defects (OC). In response, an innovative approach utilizing pre‐designed bioink modular units one‐step immediate implantation is proposed, circumventing the need prior cultivation. The resulting printed scaffold not only accurately reproduces three‐layer structure material gradient but also attains impressive compressive strength (6.3 MPa) through reinforcement hydroxyapatite nanofibers establishment chemical bonds with hydrogels. Moreover, integrates capturing homing layers on bottom top via crosslinking aptamer loading poly (lactic‐co‐glycolic acid) (PLGA) nanospheres encapsulated stromal cell‐derived factor‐1α (SDF‐1α), respectively. This design enables specific capture bone marrow mesenchymal cells (BMSCs) vivo interaction, followed by their mobilization to home hyaline layer chemotaxis SDF‐1α concentration gradient. Within scaffold's microenvironment, these BMSCs undergo differentiation into distinct each layer, effectively contributing repair OC rabbits.

Язык: Английский

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3D printed PCL/GelMA biphasic scaffold boosts cartilage regeneration using co-culture of mesenchymal stem cells and chondrocytes: In vivo study

Materials & Design, Год журнала: 2021, Номер 210, С. 110065 - 110065

Опубликована: Авг. 23, 2021

Cartilage injury is difficult to self-repair, which poses a challenge scaffold design and selection of seeded cells for cartilage tissue engineering. In this study, we presented biphasic consisting cells-encapsulated biodegradable gelatin methacrylate (GelMA) hydrogel on the upper layer macroporous poly(ε-caprolactone) (PCL) filled with GelMA lower support regeneration using co-culture bone marrow mesenchyml stem (BMSCs) costal chondrocytes (CChon). We determined that synergistic effect BMSCs was sufficient induce chondrogenesis, but better under TGF-β3 supplement. Memorably, when implanted in rat osteochondral defect model 12 weeks, PCL/GelMA scaffolds showed excellent regenerative capability, enabled Young's modulus engineered comparable native cartilage. Furthermore, implantation resulted less mechanical pain than defects by gait analysis. Together, both vitro vivo results demonstrated promising regeneration, pave new way solve key issues clinical repair.

Язык: Английский

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The Application of Bioreactors for Cartilage Tissue Engineering: Advances, Limitations, and Future Perspectives

Stem Cells International, Год журнала: 2021, Номер 2021, С. 1 - 13

Опубликована: Янв. 21, 2021

Tissue engineering (TE) has brought new hope for articular cartilage regeneration, as TE can provide structural and functional substitutes native tissues. The basic elements of involve scaffolds, seeded cells, biochemical biomechanical stimuli. However, there are some limitations TE; what most important is that static cell culture on scaffolds cannot simulate the physiological environment required development natural cartilage. Recently, bioreactors have been used to physical mechanical during This review aims an overview concepts, categories, applications with emphasis design various bioreactor systems.

Язык: Английский

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Solubilized Cartilage ECM Facilitates the Recruitment and Chondrogenesis of Endogenous BMSCs in Collagen Scaffolds for Enhancing Microfracture Treatment

ACS Applied Materials & Interfaces, Год журнала: 2021, Номер 13(21), С. 24553 - 24564

Опубликована: Май 20, 2021

Articular cartilage has very poor intrinsic healing ability and its repair remains a significant clinical challenge. To promote neocartilage regeneration, we fabricated two collagen (Col) scaffolds functionalized with porcine decellularized extracellular matrix (dECM) in the forms of particle solution named pE-Col sE-Col, respectively. Their differences were systematically compared, including biochemical compositions, scaffold properties, cell–material interactions, situ regeneration. While it is demonstrated that both dECM could enhance cell recruitment, proliferation, chondrogenesis bone marrow stem cells (BMSCs) vitro, better performance was seen sE-Col group, which quickly provide more favorable chondrogenic microenvironment for endogenous BMSCs. The superiority also proved by our vivo study, showed achieved structural hyaline-like formation subchondral compared to scaffold, according gross morphology, biological assessment, micro-CT imaging analysis. Together, this study suggests holds great potential developing one-step microfracture-based strategy reminds us despite being promising biomaterial tissue engineering, optimization proper processing methodology would be crucial consideration future design dECM-based articular

Язык: Английский

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Engineering the dynamics of biophysical cues in supramolecular hydrogels to facile control stem cell chondrogenesis for cartilage regeneration

Composites Part B Engineering, Год журнала: 2022, Номер 250, С. 110429 - 110429

Опубликована: Ноя. 18, 2022

Язык: Английский

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Advancing drug delivery to articular cartilage: From single to multiple strategies

Acta Pharmaceutica Sinica B, Год журнала: 2022, Номер 13(10), С. 4127 - 4148

Опубликована: Ноя. 25, 2022

Язык: Английский

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Microenvironmentally optimized 3D-printed TGFβ-functionalized scaffolds facilitate endogenous cartilage regeneration in sheep

Acta Biomaterialia, Год журнала: 2022, Номер 150, С. 181 - 198

Опубликована: Июль 25, 2022

Язык: Английский

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