Articular cartilage repair biomaterials: strategies and applications

Materials Today Bio, Год журнала: 2024, Номер 24, С. 100948 - 100948

Опубликована: Янв. 5, 2024

Articular cartilage injury is a frequent worldwide disease, while effective treatment urgently needed. Due to lack of blood vessels and nerves, the ability self-repair limited. Despite availability various clinical treatments, unfavorable prognoses complications remain prevalent. However, advent tissue engineering regenerative medicine has generated considerable interests in using biomaterials for articular repair. Nevertheless, there remains notable scarcity comprehensive reviews that provide an in-depth exploration strategies applications. Herein, we present overview primary bioactive substances from perspective repair cartilage. The include regeneration, substitution, immunization. We comprehensively delineate influence mechanically supportive scaffolds on cellular behavior, shedding light emerging scaffold technologies, including stimuli-responsive smart scaffolds, 3D-printed bionic scaffolds. Biologically active substances, factors, stem cells, extracellular vesicles (EVs), organoids, are elucidated their roles regulating activity chondrocytes. Furthermore, composite produced industrially put into use, also explicitly presented. This review offers innovative solutions treating ailments emphasizes potential translation.

Язык: Английский

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Advancements in tissue engineering for articular cartilage regeneration

Heliyon, Год журнала: 2024, Номер 10(3), С. e25400 - e25400

Опубликована: Фев. 1, 2024

Articular cartilage injury is a prevalent clinical condition resulting from trauma, tumors, infection, osteoarthritis, and other factors. The intrinsic lack of blood vessels, nerves, lymphatic vessels within tissue severely limits its self-regenerative capacity after injury. Current treatment options, such as conservative drug therapy joint replacement, have inherent limitations. Achieving perfect regeneration repair articular remains an ongoing challenge in the field regenerative medicine. Tissue engineering has emerged key focus research, aiming to utilize cultured expanded cells combined with suitable scaffold materials create viable, functional tissues. This review article encompasses latest advancements seed cells, scaffolds, cytokines. Additionally, role stimulatory factors including cytokines growth factors, genetic techniques, biophysical stimulation, bioreactor systems, well scaffolding natural synthetic nanostructured scaffolds tissues are discussed. Finally, we also outline signaling pathways involved regeneration. Our provides valuable insights for scholars address complex problem repair.

Язык: Английский

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Recent advancements in cartilage tissue engineering innovation and translation

Nature Reviews Rheumatology, Год журнала: 2024, Номер 20(6), С. 323 - 346

Опубликована: Май 13, 2024

Язык: Английский

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Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Regenerative Biomaterials, Год журнала: 2023, Номер 11

Опубликована: Дек. 1, 2023

Abstract Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering, clinical transformation of these remained a big challenge because difficulty simulating native organs/tissues’ microenvironment. As kind natural tissue-derived biomaterials, decellularized extracellular matrix (dECM)-based have gained attention due to their unique biomimetic properties, providing specific microenvironment suitable promoting cell proliferation, migration, attachment and regulating differentiation. The medical applications dECM-based addressed critical challenges, including poor mechanical strength insufficient stability. For reconstruction damaged tissues or organs, different types composite platforms been designed mimic microenvironment, by integrating with polymer or/and syntenic adding bioactive factors. In this review, we summarized research progress regenerative medicine, highlighting challenges future perspectives related application materials.

Язык: Английский

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Biomaterial-based scaffolds in promotion of cartilage regeneration: Recent advances and emerging applications

Journal of Orthopaedic Translation, Год журнала: 2023, Номер 41, С. 54 - 62

Опубликована: Июль 1, 2023

Osteoarthritis (OA) poses a significant burden for countless individuals, inflicting relentless pain and impairing their quality of life. Although traditional treatments OA focus on management surgical interventions, they often fall short addressing the underlying cause disease. Fortunately, emerging biomaterial-based scaffolds offer hope therapy, providing immense promise cartilage regeneration in OA. These innovative are ingeniously designed to provide support mimic intricate structure natural extracellular matrix, thus stimulating damaged cartilage. In this comprehensive review, we summarize discuss current landscape Furthermore, delve into diverse range biomaterials employed construction explore cutting-edge techniques utilized fabrication. By examining both preclinical clinical studies, aim illuminate remarkable versatility untapped potential context thoroughly state research review provides valuable insights that bridge gap between scientific knowledge practical application. This is crucial clinicians researchers who strive develop go beyond symptom directly target Through analysis multidisciplinary approach, paves way translation applications, ultimately improving lives individuals suffering from shaping future orthopedic medicine.

Язык: Английский

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In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units

Bioactive Materials, Год журнала: 2023, Номер 27, С. 200 - 215

Опубликована: Апрель 10, 2023

The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial, directional and controllable differentiation mesenchymal stem cells (MSCs) into cartilage bone compartments. Emerging organoid technology offers new opportunities for regeneration. In this study, we developed gelatin-based microcryogels customized using hyaluronic acid (HA) hydroxyapatite (HYP), respectively (denoted as CH-Microcryogels OS-Microcryogels) through vivo self-assembly organoids. showed good cytocompatibility induced chondrogenic osteogenic MSCs, while also demonstrating the ability self-assemble organoids with no delamination biphasic cartilage-bone structure. Analysis by mRNA-seq that promoted inhibited inflammation, OS-Microcryogels facilitated suppressed immune response, regulating specific signaling pathways. Finally, engraftment pre-differentiated canine defects resulted spontaneous assembly an unit, simultaneous both articular subchondral bone. conclusion, novel approach generating self-assembling utilizing tailor-made presents a highly promising avenue advancing field tissue engineering.

Язык: Английский

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Cartilage lacuna-biomimetic hydrogel microspheres endowed with integrated biological signal boost endogenous articular cartilage regeneration

Bioactive Materials, Год журнала: 2024, Номер 41, С. 61 - 82

Опубликована: Июль 12, 2024

Despite numerous studies on chondrogenesis, the repair of cartilage-particularly reconstruction cartilage lacunae through an all-in-one advanced drug delivery system remains limited. In this study, we developed a lacuna-like hydrogel microsphere endowed with integrated biological signals, enabling sequential immunomodulation and endogenous articular regeneration. We first chondrogenic growth factor transforming factor-β3 (TGF-β3) into mesoporous silica nanoparticles (MSNs). Then, TGF-β3@MSNs insulin-like 1 (IGF-1) were encapsulated within microspheres made polydopamine (pDA). final step, factor-loaded MSN@pDA chitosan (CS) containing platelet-derived factor-BB (PDGF-BB) blended to produce factors loaded composite (GFs@μS) using microfluidic technology. The presence pDA reduced initial acute inflammatory response, early, robust release PDGF-BB aided in attracting stem cells. Over subsequent weeks, continuous IGF-1 TGF-β3 amplified chondrogenesis matrix formation. μS incorporated acellular extracellular (ACECM) combined polydopamine-modified polycaprolactone (PCL) structure tissue-engineered scaffold that mimicked evenly distributed matrix, resulting enhanced patellar protection. This research provides strategic pathway for optimizing ensuring prolonged microenvironmental remodeling, leading efficient

Язык: Английский

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Three‐dimensional printed biomimetic multilayer scaffolds coordinated with sleep‐related small extracellular vesicles: A strategy for extracellular matrix homeostasis and macrophage polarization to enhance osteochondral regeneration

View, Год журнала: 2024, Номер 5(2)

Опубликована: Янв. 10, 2024

Abstract Cartilage defects resulting from injury or degeneration are a common clinical problem, and due to its avascular nature, articular cartilage has poor self‐healing capacity. Three‐dimensional (3D) bioprinting attracted great attention in tissue engineering. Melatonin (MT), hormone mainly secreted at night, plays an important role repair. Small extracellular vesicles (sEV) considered ideal drug delivery vehicles MT‐sEV (sleep‐related sEV) have the potential ability promote regeneration. Here, biomimetic multilayer scaffolds were fabricated using 3D bioprinting. A double network hydrogel, composed of methacrylated hyaluronic acid gelatin methacryloyl (HG), was prepared. HG hydrogel used create layer. bone layer formed poly(ε‐caprolactone) hydroxyapatite ultralong nanowires. Additionally, two bioinks alternately printed interface The results RNA sequencing revealed regulatory mechanisms. showed promotional effects on cell migration, proliferation, chondrogenic differentiation, matrix (ECM) deposition. Moreover, altered macrophage polarization regulated expression inflammatory cytokines. In vivo experiments demonstrated that promoted These regulate immune microenvironment secretion ECM, providing promising strategy for

Язык: Английский

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Dual microparticles programmed delivery system regulating stem cell-based cartilage regeneration by cartilage-specific matrix hydrogels

Composites Part B Engineering, Год журнала: 2024, Номер 272, С. 111221 - 111221

Опубликована: Янв. 15, 2024

Bone marrow mesenchymal stem cells (BMSCs)-based engineered cartilage usually faces the significant challenge of endochondral ossification tendency in an ectopic environment because inevitable vascular infiltration during chondrogenic differentiation and developmental stages. Additionally, current scaffold-free BMSC regeneration requires a long vitro preinduction time for before vivo transplantation, which limits its application multiple defect repair. Therefore, ideal cell-based needs to meet time-dependent requirements both early late anti-angiogenic microenvironments vivo. Here, we developed cartilage-specific matrix hydrogel (CMH) with dual microparticle-based programmed delivery system (dM-PDs) dynamically regulate without preinduction. In this study, CMH scaffolds offer three-dimensional microenvironment tissue regeneration, while dM-PDs has induction function promote exerts effect stabilize cartilaginous phenotype. Stem was successfully achieved by sequentially dynamic regulation dM-PDs, TGFβ3-loaded microparticles effectively activated early-stage TGFβ/Smad signaling pathway levatinib-loaded regulated late-stage VEGF/TIMP pathway. This study demonstrates universal technique clinical defects

Язык: Английский

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Plant‐derived exosomes extracted from Lycium barbarum L. loaded with isoliquiritigenin to promote spinal cord injury repair based on 3D printed bionic scaffold

Bioengineering & Translational Medicine, Год журнала: 2024, Номер 9(4)

Опубликована: Янв. 30, 2024

Abstract Plant‐derived exosomes (PEs) possess an array of therapeutic properties, including antitumor, antiviral, and anti‐inflammatory capabilities. They are also implicated in defensive responses to pathogenic attacks. Spinal cord injuries (SCIs) regeneration represents a global medical challenge, with appropriate research concentration on three pivotal domains: neural promotion, inflammation inhibition, innovation application regenerative scaffolds. Unfortunately, the utilization PE SCI therapy remains unexplored. Herein, we isolated from traditional Chinese medicinal herb, Lycium barbarum L. discovered their inflammatory inhibition neuronal differentiation promotion Compared derived ectomesenchymal stem cells (EMSCs), demonstrated substantial enhancement differentiation. We encapsulated isoliquiritigenin (ISL)‐loaded plant‐derived (ISL@PE) within 3D‐printed bionic scaffold. The intricate construct modulated response following SCI, facilitating restoration damaged axons culminating ameliorated neurological function. This pioneering investigation proposes novel potential route for insoluble drug delivery via plant exosomes, as well repair. institutional animal care use committee number is UJS‐IACUC‐2020121602.

Язык: Английский

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