Super-low-dose functional and molecular photoacoustic microscopy DOI Creative Commons
Yachao Zhang,

Jiangbo Chen,

JIE ZHANG

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Март 17, 2022

Abstract Photoacoustic microscopy can image various biological molecules and nano-agents in vivo via low-scattering ultrasonic sensing. Insufficient sensitivity has been a long-standing obstacle for imaging of many low-absorbing chromophores with less photobleaching or toxicity, reduced perturbation to delicate organs, more choices lasers. Here, we present multi-spectral super-low-dose photoacoustic (SLD-PAM) that is up ~ 33-times sensitive than the state art. We collaboratively innovate probe design implement spectral-spatial filter. The new collects 300% acoustic signals, filter further improves signal-to-noise ratio by 200%~1100% across all optical wavelengths. SLD-PAM visualize microvessels quantify oxygen saturation (sO 2 ) 1% maximum permissible exposure, dramatically reducing any possible phototoxicity perturbation, especially eye brain. Capitalizing on high sensitivity, we, first time, direct deoxyhemoglobin (Deoxy-Hb) concentration without spectral unmixing, avoiding wavelength-dependent computational noises. show molecular reduce at least 85%. also demonstrate achieves similar quality using 80% fewer contrast agents, which greatly reduces potential biotoxicity. enables use nano-agents, small molecules, genetically-encoded biomarkers, as well low-power light sources wider spectra. believe offers powerful tool anatomical, functional, imaging.

Язык: Английский

Hepatocellular Carcinoma DOI

Previn Ganesan,

Laura Kulik

Clinics in Liver Disease, Год журнала: 2022, Номер 27(1), С. 85 - 102

Опубликована: Окт. 18, 2022

Язык: Английский

Процитировано

288
Targeted contrast agents and activatable probes for photoacoustic imaging of cancer DOI
Zhenxiang Zhao, Chelsea B. Swartchick, Jefferson Chan

и другие.

Chemical Society Reviews, Год журнала: 2022, Номер 51(3), С. 829 - 868

Опубликована: Янв. 1, 2022

Photoacoustic (PA) imaging has emerged as a powerful technique for the high resolution visualization of biological processes within deep tissue. Through development and application exogenous targeted contrast agents activatable probes that can respond to given cancer biomarker, researchers image molecular events

Язык: Английский

Процитировано

97
Second Near‐Infrared Plasmonic Nanomaterials for Photoacoustic Imaging and Photothermal Therapy DOI Open Access

Tingjun Yan,

Mengyao Su,

Zhimin Wang

и другие.

Small, Год журнала: 2023, Номер 19(30)

Опубликована: Апрель 14, 2023

Abstract Photoacoustic imaging (PAI) and imaging‐guided photothermal therapy (PTT) in the second near‐infrared window (NIR‐II, 1000–1700 nm) have received increasing attention owing to their advantages of greater penetration depth higher signal‐to‐noise ratio. Plasmonic nanomaterials with tunable optical properties strong light absorption provide an alternative dye molecules, showing great prospects for phototheranostic applications. In this review, research progress principally modulating plasmonic nanomaterials, especially affecting parameters such as size, morphology, surface chemical modification, is introduced. The commonly used NIR‐II window, including noble metals, semiconductors, heterostructures, are then summarized. addition, biomedical applications these PAI PTT phototheranostics highlighted. Finally, perspectives challenges advancing practical use clinical translation discussed.

Язык: Английский

Процитировано

46
Organic Semiconducting Luminophores for Near‐Infrared Afterglow, Chemiluminescence, and Bioluminescence Imaging DOI
Xiaozhen Li, Chao Yin, Si Si Liew

и другие.

Advanced Functional Materials, Год журнала: 2021, Номер 31(46)

Опубликована: Авг. 6, 2021

Abstract Optical imaging has played a pivotal role in deciphering vivo bioinformatics but is limited by shallow penetration depth and poor performance owing to interfering tissue autofluorescence induced concurrent photoexcitation. The emergence of near‐infrared (NIR) self‐luminescence independent real‐time irradiation timely addressed these problems. There are two main kinds self‐luminescent agents, namely inorganic organic luminophores. Inorganic luminophores usually suffer from long‐term biotoxicity concerns resulting potential heavy‐metal ions leakage nonbiodegradability, which hinders their further translational application. In contrast, luminophores, especially semiconducting (OSLs) with good biodegradable potential, tunable design, outstanding optical properties, preferred biological applications. This review summarizes the recent progress OSLs used NIR afterglow, chemiluminescence, bioluminescence imaging. Molecular manipulation nanoengineering approaches discussed, emphasis on strategies that can extend emission wavelength visible range amplify luminescence signals. concludes discussion current challenges possible solutions field.

Язык: Английский

Процитировано

84
Combination of Ablation and Immunotherapy for Hepatocellular Carcinoma: Where We Are and Where to Go DOI Creative Commons
Kunpeng Wang,

Cong Wang,

Hao Jiang

и другие.

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Дек. 15, 2021

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide and increasing in incidence. Local ablative therapy plays a role HCC treatment. Radiofrequency (RFA) one first-line therapies for early local ablation. Other ablation techniques (e.g., microwave ablation, cryoablation, irreversible electroporation, phototherapy.) have been extensively explored clinical trials or cell/animal studies but not yet established as standard treatment applied clinically. On hand, single may meet needs. other can stimulate systemic immune effects. The combination strategy immunotherapy reasonable. In this review, we briefly summarized current status progress HCC. effects strategies therapy, especially synergistic based on biomedical materials, were discussed. This review hoped to provide references future researches arrive promising new era

Язык: Английский

Процитировано

63
Nanophotonic-enhanced photoacoustic imaging for brain tumor detection DOI Creative Commons

Ali Rizwan,

Badrinathan Sridharan,

Jin Hyeong Park

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 5, 2025

Photoacoustic brain imaging (PABI) has emerged as a promising biomedical modality, combining high contrast of optical with deep tissue penetration ultrasound imaging. This review explores the application photoacoustic in tumor imaging, highlighting synergy between nanomaterials and state art techniques to achieve high-resolution deeper tissues. PABI leverages effect, where absorbed light energy causes thermoelastic expansion, generating waves that are detected converted into images. technique enables precise diagnosis, therapy monitoring, enhanced clinical screening, specifically management complex diseases such breast cancer, lymphatic disorder, neurological conditions. Despite integration agents radiation, providing comprehensive overview current methodologies, major obstacles treatment, future directions for improving diagnostic therapeutic outcomes. The underscores significance robust research tool medical method, potential revolutionize disease diagnosis treatment.

Язык: Английский

Процитировано

2
Functional photoacoustic microscopy of hemodynamics: a review DOI
Chao Liu, Lidai Wang

Biomedical Engineering Letters, Год журнала: 2022, Номер 12(2), С. 97 - 124

Опубликована: Апрель 10, 2022

Язык: Английский

Процитировано

35
Video-Rate Dual-Modal Wide-Beam Harmonic Ultrasound and Photoacoustic Computed Tomography DOI
Yachao Zhang, Yue Wang, Puxiang Lai

и другие.

IEEE Transactions on Medical Imaging, Год журнала: 2021, Номер 41(3), С. 727 - 736

Опубликована: Окт. 25, 2021

Dual-modal ultrasound (US) and photoacoustic (PA) imaging has tremendous advantages in biomedical applications, such as pharmacokinetics, cancer screening, imaging-guided therapy. Compared with ring-shaped arrays, a linear piezoelectric transducer array applies to more anatomical sites been widely used US/PA imaging. However, the may limit quality due narrow bandwidth, partial detection view, or sparse spatial sampling. To meet clinic demand of high-quality transducer, we develop dual-modal wide-beam harmonic (WBHUS) computed tomography at video rate. The US employs pulse phase inversion reduce clutters improve resolution. Wide-beam transmission can shorten scanning times by 267% enables 20-Hz rate, which minimize motion artifacts in vivo does not only provide accurate references for locating PA features but also reduces images. improved image allows us acquire high-resolution structures deep tissue without labeling. fast-imaging speed visualizing interventional procedures monitoring pulsations thoracic aorta radial artery real-time. video-rate single-shot use clinical-grade linear-array thus be readily implemented clinical

Язык: Английский

Процитировано

24
Adaptive dual-speed ultrasound and photoacoustic computed tomography DOI Creative Commons
Yachao Zhang, Lidai Wang

Photoacoustics, Год журнала: 2022, Номер 27, С. 100380 - 100380

Опубликована: Июнь 9, 2022

Full-ring dual-modal ultrasound and photoacoustic computed tomography has unique advantages of nearly isotropic spatial resolution, complementary contrast, deep penetration, full-view detection. However, the imaging quality may be deteriorated by inaccurate sound speed estimation. Automatic determining compensation for been a long-standing problem in image reconstruction. Here, we present new adaptive dual-speed (ADS-USPACT) to address this challenge. The system features coverage (360°), high-speed (10-Hz), automated dual correction, synergistic high quality. To correct speed, develop two-compartment method that can automatically segment sample boundary search optimal based on rich ultrasonic pulse-echo signals. does not require operator's intervention. We validate technique numerical simulation, phantom study, vivo experiments. ADS-USPACT represents significant progress imaging.

Язык: Английский

Процитировано

17
MRI-Based Multifunctional Nanoliposomes for Enhanced HCC Therapy and Diagnosis DOI
Jingxin Sun, Zhehao Jin, Yong Jun Jin

и другие.

Molecular Pharmaceutics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

The morbidity and mortality rates of hepatocellular carcinoma (HCC) are high continue to increase. antitumor effects single therapies limited because tumor heterogeneity drug resistance, the lack real-time monitoring progression during treatment process leads poor therapeutic outcomes. Therefore, novel nanodelivery platforms combining therapy diagnosis have garnered extensive attention. In this study, we developed a multifunctional vector, LPSD-DOX/siRNA, which was loaded with oleic acid-modified superparamagnetic iron oxide nanoparticles (OA-SPION) doxorubicin (DOX), further modified by DOTAP carry small interfering RNA targeting phosphatidylinositol proteoglycan-3 (Glypican-3, GPC3) (siRNA-GPC3). These components were utilized for combined HCC magnetic resonance imaging. LPSD-DOX/siRNA exhibited loading, gene transfection efficiency, low toxicity. Pharmacokinetic in vivo distribution experiments showed that significantly prolonged circulation time DOX enhanced accumulation at site. Magnetic imaging demonstrated can serve as T2 contrast agent enhance between site other tissues facilitate tissues. Antitumor revealed promoted inhibiting expression GPC3 protein HepG2 cell-transplanted tumors, increased apoptosis. conclusion, serves promising strategy combination HCC, significant potential therapy.

Язык: Английский

Процитировано

0