Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Март 17, 2022
Abstract
Photoacoustic
microscopy
can
image
various
biological
molecules
and
nano-agents
in
vivo
via
low-scattering
ultrasonic
sensing.
Insufficient
sensitivity
has
been
a
long-standing
obstacle
for
imaging
of
many
low-absorbing
chromophores
with
less
photobleaching
or
toxicity,
reduced
perturbation
to
delicate
organs,
more
choices
lasers.
Here,
we
present
multi-spectral
super-low-dose
photoacoustic
(SLD-PAM)
that
is
up
~
33-times
sensitive
than
the
state
art.
We
collaboratively
innovate
probe
design
implement
spectral-spatial
filter.
The
new
collects
300%
acoustic
signals,
filter
further
improves
signal-to-noise
ratio
by
200%~1100%
across
all
optical
wavelengths.
SLD-PAM
visualize
microvessels
quantify
oxygen
saturation
(sO
2
)
1%
maximum
permissible
exposure,
dramatically
reducing
any
possible
phototoxicity
perturbation,
especially
eye
brain.
Capitalizing
on
high
sensitivity,
we,
first
time,
direct
deoxyhemoglobin
(Deoxy-Hb)
concentration
without
spectral
unmixing,
avoiding
wavelength-dependent
computational
noises.
show
molecular
reduce
at
least
85%.
also
demonstrate
achieves
similar
quality
using
80%
fewer
contrast
agents,
which
greatly
reduces
potential
biotoxicity.
enables
use
nano-agents,
small
molecules,
genetically-encoded
biomarkers,
as
well
low-power
light
sources
wider
spectra.
believe
offers
powerful
tool
anatomical,
functional,
imaging.
Chemical Society Reviews,
Год журнала:
2022,
Номер
51(3), С. 829 - 868
Опубликована: Янв. 1, 2022
Photoacoustic
(PA)
imaging
has
emerged
as
a
powerful
technique
for
the
high
resolution
visualization
of
biological
processes
within
deep
tissue.
Through
development
and
application
exogenous
targeted
contrast
agents
activatable
probes
that
can
respond
to
given
cancer
biomarker,
researchers
image
molecular
events
Abstract
Photoacoustic
imaging
(PAI)
and
imaging‐guided
photothermal
therapy
(PTT)
in
the
second
near‐infrared
window
(NIR‐II,
1000–1700
nm)
have
received
increasing
attention
owing
to
their
advantages
of
greater
penetration
depth
higher
signal‐to‐noise
ratio.
Plasmonic
nanomaterials
with
tunable
optical
properties
strong
light
absorption
provide
an
alternative
dye
molecules,
showing
great
prospects
for
phototheranostic
applications.
In
this
review,
research
progress
principally
modulating
plasmonic
nanomaterials,
especially
affecting
parameters
such
as
size,
morphology,
surface
chemical
modification,
is
introduced.
The
commonly
used
NIR‐II
window,
including
noble
metals,
semiconductors,
heterostructures,
are
then
summarized.
addition,
biomedical
applications
these
PAI
PTT
phototheranostics
highlighted.
Finally,
perspectives
challenges
advancing
practical
use
clinical
translation
discussed.
Advanced Functional Materials,
Год журнала:
2021,
Номер
31(46)
Опубликована: Авг. 6, 2021
Abstract
Optical
imaging
has
played
a
pivotal
role
in
deciphering
vivo
bioinformatics
but
is
limited
by
shallow
penetration
depth
and
poor
performance
owing
to
interfering
tissue
autofluorescence
induced
concurrent
photoexcitation.
The
emergence
of
near‐infrared
(NIR)
self‐luminescence
independent
real‐time
irradiation
timely
addressed
these
problems.
There
are
two
main
kinds
self‐luminescent
agents,
namely
inorganic
organic
luminophores.
Inorganic
luminophores
usually
suffer
from
long‐term
biotoxicity
concerns
resulting
potential
heavy‐metal
ions
leakage
nonbiodegradability,
which
hinders
their
further
translational
application.
In
contrast,
luminophores,
especially
semiconducting
(OSLs)
with
good
biodegradable
potential,
tunable
design,
outstanding
optical
properties,
preferred
biological
applications.
This
review
summarizes
the
recent
progress
OSLs
used
NIR
afterglow,
chemiluminescence,
bioluminescence
imaging.
Molecular
manipulation
nanoengineering
approaches
discussed,
emphasis
on
strategies
that
can
extend
emission
wavelength
visible
range
amplify
luminescence
signals.
concludes
discussion
current
challenges
possible
solutions
field.
Frontiers in Immunology,
Год журнала:
2021,
Номер
12
Опубликована: Дек. 15, 2021
Hepatocellular
carcinoma
(HCC)
is
the
third
leading
cause
of
cancer-related
deaths
worldwide
and
increasing
in
incidence.
Local
ablative
therapy
plays
a
role
HCC
treatment.
Radiofrequency
(RFA)
one
first-line
therapies
for
early
local
ablation.
Other
ablation
techniques
(e.g.,
microwave
ablation,
cryoablation,
irreversible
electroporation,
phototherapy.)
have
been
extensively
explored
clinical
trials
or
cell/animal
studies
but
not
yet
established
as
standard
treatment
applied
clinically.
On
hand,
single
may
meet
needs.
other
can
stimulate
systemic
immune
effects.
The
combination
strategy
immunotherapy
reasonable.
In
this
review,
we
briefly
summarized
current
status
progress
HCC.
effects
strategies
therapy,
especially
synergistic
based
on
biomedical
materials,
were
discussed.
This
review
hoped
to
provide
references
future
researches
arrive
promising
new
era
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 5, 2025
Photoacoustic
brain
imaging
(PABI)
has
emerged
as
a
promising
biomedical
modality,
combining
high
contrast
of
optical
with
deep
tissue
penetration
ultrasound
imaging.
This
review
explores
the
application
photoacoustic
in
tumor
imaging,
highlighting
synergy
between
nanomaterials
and
state
art
techniques
to
achieve
high-resolution
deeper
tissues.
PABI
leverages
effect,
where
absorbed
light
energy
causes
thermoelastic
expansion,
generating
waves
that
are
detected
converted
into
images.
technique
enables
precise
diagnosis,
therapy
monitoring,
enhanced
clinical
screening,
specifically
management
complex
diseases
such
breast
cancer,
lymphatic
disorder,
neurological
conditions.
Despite
integration
agents
radiation,
providing
comprehensive
overview
current
methodologies,
major
obstacles
treatment,
future
directions
for
improving
diagnostic
therapeutic
outcomes.
The
underscores
significance
robust
research
tool
medical
method,
potential
revolutionize
disease
diagnosis
treatment.
IEEE Transactions on Medical Imaging,
Год журнала:
2021,
Номер
41(3), С. 727 - 736
Опубликована: Окт. 25, 2021
Dual-modal
ultrasound
(US)
and
photoacoustic
(PA)
imaging
has
tremendous
advantages
in
biomedical
applications,
such
as
pharmacokinetics,
cancer
screening,
imaging-guided
therapy.
Compared
with
ring-shaped
arrays,
a
linear
piezoelectric
transducer
array
applies
to
more
anatomical
sites
been
widely
used
US/PA
imaging.
However,
the
may
limit
quality
due
narrow
bandwidth,
partial
detection
view,
or
sparse
spatial
sampling.
To
meet
clinic
demand
of
high-quality
transducer,
we
develop
dual-modal
wide-beam
harmonic
(WBHUS)
computed
tomography
at
video
rate.
The
US
employs
pulse
phase
inversion
reduce
clutters
improve
resolution.
Wide-beam
transmission
can
shorten
scanning
times
by
267%
enables
20-Hz
rate,
which
minimize
motion
artifacts
in
vivo
does
not
only
provide
accurate
references
for
locating
PA
features
but
also
reduces
images.
improved
image
allows
us
acquire
high-resolution
structures
deep
tissue
without
labeling.
fast-imaging
speed
visualizing
interventional
procedures
monitoring
pulsations
thoracic
aorta
radial
artery
real-time.
video-rate
single-shot
use
clinical-grade
linear-array
thus
be
readily
implemented
clinical
Photoacoustics,
Год журнала:
2022,
Номер
27, С. 100380 - 100380
Опубликована: Июнь 9, 2022
Full-ring
dual-modal
ultrasound
and
photoacoustic
computed
tomography
has
unique
advantages
of
nearly
isotropic
spatial
resolution,
complementary
contrast,
deep
penetration,
full-view
detection.
However,
the
imaging
quality
may
be
deteriorated
by
inaccurate
sound
speed
estimation.
Automatic
determining
compensation
for
been
a
long-standing
problem
in
image
reconstruction.
Here,
we
present
new
adaptive
dual-speed
(ADS-USPACT)
to
address
this
challenge.
The
system
features
coverage
(360°),
high-speed
(10-Hz),
automated
dual
correction,
synergistic
high
quality.
To
correct
speed,
develop
two-compartment
method
that
can
automatically
segment
sample
boundary
search
optimal
based
on
rich
ultrasonic
pulse-echo
signals.
does
not
require
operator's
intervention.
We
validate
technique
numerical
simulation,
phantom
study,
vivo
experiments.
ADS-USPACT
represents
significant
progress
imaging.
Molecular Pharmaceutics,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 14, 2025
The
morbidity
and
mortality
rates
of
hepatocellular
carcinoma
(HCC)
are
high
continue
to
increase.
antitumor
effects
single
therapies
limited
because
tumor
heterogeneity
drug
resistance,
the
lack
real-time
monitoring
progression
during
treatment
process
leads
poor
therapeutic
outcomes.
Therefore,
novel
nanodelivery
platforms
combining
therapy
diagnosis
have
garnered
extensive
attention.
In
this
study,
we
developed
a
multifunctional
vector,
LPSD-DOX/siRNA,
which
was
loaded
with
oleic
acid-modified
superparamagnetic
iron
oxide
nanoparticles
(OA-SPION)
doxorubicin
(DOX),
further
modified
by
DOTAP
carry
small
interfering
RNA
targeting
phosphatidylinositol
proteoglycan-3
(Glypican-3,
GPC3)
(siRNA-GPC3).
These
components
were
utilized
for
combined
HCC
magnetic
resonance
imaging.
LPSD-DOX/siRNA
exhibited
loading,
gene
transfection
efficiency,
low
toxicity.
Pharmacokinetic
in
vivo
distribution
experiments
showed
that
significantly
prolonged
circulation
time
DOX
enhanced
accumulation
at
site.
Magnetic
imaging
demonstrated
can
serve
as
T2
contrast
agent
enhance
between
site
other
tissues
facilitate
tissues.
Antitumor
revealed
promoted
inhibiting
expression
GPC3
protein
HepG2
cell-transplanted
tumors,
increased
apoptosis.
conclusion,
serves
promising
strategy
combination
HCC,
significant
potential
therapy.