Physics of Life Reviews, Год журнала: 2024, Номер 50, С. 13 - 26
Опубликована: Май 14, 2024
Язык: Английский
Physics of Life Reviews, Год журнала: 2024, Номер 50, С. 13 - 26
Опубликована: Май 14, 2024
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2135 - 2135
Опубликована: Фев. 10, 2024
Contact inhibition (CI) represents a crucial tumor-suppressive mechanism responsible for controlling the unbridled growth of cells, thus preventing formation cancerous tissues. CI can be further categorized into two distinct yet interrelated components: locomotion (CIL) and proliferation (CIP). These components have historically been viewed as separate processes, but emerging research suggests that they may regulated by both shared pathways. Specifically, recent studies indicated CIP CIL utilize mechanotransduction pathways, process involves cells sensing responding to mechanical forces. This review article describes role in CI, shedding light on how forces regulate CIP. Emphasis is placed filamin A (FLNA)-mediated mechanotransduction, elucidating FLNA senses translates them biochemical signals cell proliferation. In addition FLNA, trans-acting factors (TAFs), which are proteins or regulatory RNAs capable directly indirectly binding specific DNA sequences distant genes gene expression, emerge sensitive players signaling pathways CI. presents methods identifying these TAF profiling associated changes chromatin structure, offering valuable insights other biological functions mediated mechanotransduction. Finally, it addresses unanswered questions fields delineates their possible future directions.
Язык: Английский
Процитировано
8Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 2, 2025
Язык: Английский
Процитировано
1Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 171, С. 116117 - 116117
Опубликована: Янв. 3, 2024
Tumor angiogenesis is one of the typical hallmarks tumor occurrence and development, neovascularization also exhibits distinct characteristics from normal blood vessels. As number cells matrix inside increases, biomechanical force enhanced, specifically manifested as solid stress, fluid stiffness, topology. This mechanical microenvironment provides shelter for tumors intensifies angiogenesis, providing oxygen nutritional support progression. During emerges, which in turn feeds back to regulate progression, including biochemical signals can angiogenesis. Blood vessels possess inherent sensitivity stimuli, but compared extensive research on signal regulation, study regulation by remains relatively scarce. Biomechanical forces affect phenotypic signaling pathways vessels, directly regulating Meanwhile, they indirectly causing an imbalance affecting stromal cell function. Understanding regulatory mechanism beneficial better identifying even taming involved new therapeutic targets vascular normalization. Therefore, we summarized composition their direct or indirect neovascularization. In addition, this review discussed use combination with anti-angiogenic therapies treatment tumors, triggered delivery systems.
Язык: Английский
Процитировано
7Materials Today Bio, Год журнала: 2024, Номер 26, С. 101058 - 101058
Опубликована: Апрель 17, 2024
Biomechanical cues could effectively govern cell gene expression to direct the differentiation of specific stem lineage. Recently, medium viscosity has emerged as a significant mechanical stimulator that regulates cellular properties and various physiological functions. However, whether can regulate human mesenchymal cells (hMSCs) trigger osteogenic remains uncertain. The mechanism by which sense respond changes in viscosity, promote lineage, elusive. In this study, we demonstrated hMSCs, cultured high-viscosity medium, exhibited larger spreading area higher intracellular tension, correlated with elevated formation actin stress fibers focal adhesion maturation. Furthermore, these observed hMSCs were associated activation TRPV4 (transient receptor potential vanilloid sub-type 4) channels on membrane. This feedback loop among activation, tension results calcium influx, subsequently promotes nuclear localization NFATc1 (nuclear factor activated T 1). Concomitantly, induced deformation promoted YAP (YES-associated protein). concurrent significantly enhanced alkaline phosphatase (ALP) for pre-osteogenic activity. Taken together, findings provide more comprehensive view how viscosity-induced alterations biomechanical MSCs impact osteogenesis-related genes, ultimately
Язык: Английский
Процитировано
6EBioMedicine, Год журнала: 2024, Номер 110, С. 105412 - 105412
Опубликована: Окт. 31, 2024
Язык: Английский
Процитировано
6Clinical and Translational Medicine, Год журнала: 2024, Номер 14(5)
Опубликована: Май 1, 2024
Abstract Background Mucinous colorectal adenocarcinoma (MCA) is a distinct subtype of cancer (CRC) with the most aggressive pattern, but effective treatment MCA remains challenge due to its vague pathological characteristics. An in‐depth understanding transcriptional dynamics at cellular level critical for developing specialised strategies. Methods We integrated single‐cell RNA sequencing and spatial transcriptomics data systematically profile tumor microenvironment (TME), particularly interactome stromal immune cells. In addition, three‐dimensional bioprinting technique, canonical ex vivo co‐culture system, immunofluorescence staining were further applied validate communication networks within TME. Results This study identified crucial intercellular interactions that engaged in pathogenesis. found increased infiltration FGF7 + / THBS1 myofibroblasts tissues decreased expression genes associated leukocyte‐mediated immunity T cell activation, suggesting role these cells regulating immunosuppressive MS4A4A macrophages exhibit M2‐phenotype enriched tumoral niche high was poor prognosis cohort data. The ligand‐receptor‐based analysis revealed tight interaction MUC1 malignant ZEB1 endothelial cells, providing mechanistic information angiogenesis molecular targets subsequent translational applications. Conclusions Our provides novel insights into communications among tumour are significantly TME during progression, presenting potential prognostic biomarkers therapeutic strategies MCA. Key points Tumour profiling developed. interplay promote development. M2 phenotype endotheliocytes engage EndMT process
Язык: Английский
Процитировано
5Soft Matter, Год журнала: 2023, Номер 19(13), С. 2438 - 2445
Опубликована: Янв. 1, 2023
Using viscoelastically-tunable polyacrylamide hydrogels, we show that macrophage morphology and phagocytosis depend on substrate loss modulus, indicating viscoelasticity may be an important design parameter in immunomodulatory biomaterials.
Язык: Английский
Процитировано
12Biomaterials Advances, Год журнала: 2023, Номер 157, С. 213738 - 213738
Опубликована: Дек. 22, 2023
Язык: Английский
Процитировано
12International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(9), С. 8239 - 8239
Опубликована: Май 4, 2023
Healing after tooth extraction involves a series of reparative processes affecting both alveolar bone and soft tissues. The aim the present study was to investigate whether activation molecular signals during healing process confers regenerative advantage socket tissue (ESsT) at 8 weeks healing. Compared subepithelial connective graft (CTG), qRT-PCR analyses revealed dramatic enrichment ESsT in osteogenic differentiation markers. However, CTG shared characteristics nonspecialized by expressing comparable levels genes encoding abundant extracellular matrix (ECM) proteins. Genes transforming growth factor-β1 (TGF-β1) its receptors were strongly enriched CTG, whereas transcript for insulin-like factor-1 (IGF-1) showed significantly high expression Mechanical stimulation, means cyclic strain or stiffness applied primary cells (ESsT-C) fibroblasts (CTG-F) extracted from samples, that stress-induced TGF-β1 not exceeding 2.3 ng/mL, as measured ELISA, combination with IGF-1 up 2.5 ng/mL able induce potential ESsT-Cs. stiff matrices (50 kPa), upregulating 6.6 caused downregulation gene In CTG-Fs, endogenous ≥ 4.6 likely responsible complete lack osteogenesis. Treatment ESsT-Cs proved that, specific concentrations, two factors exhibited either an inductive-synergistic suppressive activity, thus determining mineralization Taken together, our data warrant clinical exploration augmentative procedures dental implant placement surgeries.
Язык: Английский
Процитировано
11International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(3), С. 1748 - 1748
Опубликована: Фев. 1, 2024
Ventilator-induced lung injury (VILI) during mechanical ventilation (MV) has been attributed to airway remodeling involving increased smooth muscle cells (ASMCs), but the underlying mechanism is not fully understood. Thus, we aimed investigate whether MV-associated high stretch (>10% strain) could modulate mechanosensitive Piezo1 expression and thereby alter cell migration of ASMCs as a potential pathway in VILI. C57BL/6 mice were subjected MV at tidal volume (VT, 18 mL/kg, 3 h) (13% strain, 0.5 Hz, 72 h), respectively. Subsequently, or evaluated for integrin mRNA by immunohistochemical staining quantitative PCR (qPCR), adhesion transwell assays. Cells either treated with siRNA, Piezo1-eGFP, knockin, Y27632, blebbistatin regulate inhibit Rho-associated kinase (ROCK) signaling prior assessment. We found that situ tissue, αVβ1 isolated from all reduced primary (pASMCs) was greatly enhanced. Similarly, line mouse (mASMCs) cultured vitro showed Interestingly, such effects on be induced abolished/reversed down/up-regulation inhibition ROCK signaling. High associated appears modulator can, thus, promote therapeutic MV. This may novel detrimental MV, and, therefore, intervention target treat
Язык: Английский
Процитировано
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