Nanotechnology Strategies for Plant Genetic Engineering

Advanced Materials, Год журнала: 2021, Номер 34(7)

Опубликована: Окт. 26, 2021

Plant genetic engineering is essential for improving crop yield, quality, and resistance to abiotic/biotic stresses sustainable agriculture. Agrobacterium-, biolistic bombardment-, electroporation-, poly(ethylene glycol) (PEG)-mediated genetic-transformation systems are extensively used in plant engineering. However, these have limitations, including species dependency, destruction of tissues, low transformation efficiency, high cost. Recently, nanotechnology-based gene-delivery methods been developed transformation. This nanostrategy shows excellent good biocompatibility, adequate protection exogenous nucleic acids, the potential regeneration. nanomaterial-mediated system plants still its infancy, there many challenges broad applications. Herein, conventional techniques briefly discussed. After that, progress development nanomaterial-based considered. CRISPR-Cas-mediated genome editing combined applications with nanotechnology also The conceptual innovations, methods, practical summarized herein will be beneficial promoting modern

Язык: Английский

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Living Materials for Regenerative Medicine

Engineered Regeneration, Год журнала: 2021, Номер 2, С. 96 - 104

Опубликована: Янв. 1, 2021

Regenerative medicine has been attracting tremendous attention during the past few decades because it is promising in overcoming limitations of donor shortage and immune complications direct transplantations. The ongoing progress this field calls for rapid growth living materials, which consist live biological agents can be designed together with synthetic materials to meet application demands regenerative medicine. In review, we present a summary state-of-the-art that are applied We first introduce advanced engineering approaches employed prepare containing cells, typically including genetic engineering, cell coating, microfluidics, bioprinting, etc. Afterwards, enumerate different aspects medicine, tissue scaffold, therapy, models, so on. Finally, give concise conclusion provide perspective field.

Язык: Английский

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The Application of the CRISPR-Cas System in Antibiotic Resistance

Infection and Drug Resistance, Год журнала: 2022, Номер Volume 15, С. 4155 - 4168

Опубликована: Авг. 1, 2022

The emergence and global epidemic of antimicrobial resistance (AMR) poses a serious threat to public health in recent years. AMR genes are shared between bacterial pathogens mainly via horizontal gene transfer (HGT) on mobile genetic elements (MGEs), thereby accelerating the spread increasing burden drug resistance. There is an urgent need develop new strategies control infections clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas) RNA-guided adaptive immune system prokaryotes that recognizes defends against invasive such as phages plasmids. Because its specifically target cleave DNA sequences encoding antibiotic genes, CRISPR/Cas has been developed into gene-editing tool for prevention CRISPR-Cas plays potentially important role controlling limiting In this review, we will introduce structure working mechanism systems, followed by delivery strategies, then focus relationship CRISPR-Cas. Moreover, challenges prospects research field discussed, providing reference

Язык: Английский

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Metal-organic frameworks (MOFs) as biomolecules drug delivery systems for anticancer purposes

European Journal of Medicinal Chemistry, Год журнала: 2022, Номер 244, С. 114801 - 114801

Опубликована: Окт. 4, 2022

Язык: Английский

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Nanotechnological Approaches in Prostate Cancer Therapy: Integration of engineering and biology

Nano Today, Год журнала: 2022, Номер 45, С. 101532 - 101532

Опубликована: Июль 12, 2022

Язык: Английский

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Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

Journal of nanostructure in chemistry, Год журнала: 2022, Номер 12(5), С. 833 - 864

Опубликована: Фев. 14, 2022

Язык: Английский

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Cancer immunotherapy by immune checkpoint blockade and its advanced application using bio-nanomaterials

Seminars in Cancer Biology, Год журнала: 2022, Номер 86, С. 909 - 922

Опубликована: Фев. 16, 2022

Язык: Английский

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Application of CRISPR-Cas9 genome editing technology in various fields: A review

Narra J, Год журнала: 2023, Номер 3(2), С. e184 - e184

Опубликована: Авг. 27, 2023

CRISPR-Cas9 has emerged as a revolutionary tool that enables precise and efficient modifications of the genetic material. This review provides comprehensive overview technology its applications in genome editing. We begin by describing fundamental principles technology, explaining how system utilizes single guide RNA (sgRNA) to direct Cas9 nuclease specific DNA sequences genome, resulting targeted double-stranded breaks. In this review, we provide in-depth explorations agriculture, medicine, environmental sciences, fisheries, nanotechnology, bioinformatics, biotechnology. also highlight potential, ongoing research, ethical considerations controversies surrounding use. might contribute understanding implications various fields, paving way for future developments responsible transformative technology.

Язык: Английский

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A review on CRISPR/Cas: a versatile tool for cancer screening, diagnosis, and clinic treatment

Functional & Integrative Genomics, Год журнала: 2023, Номер 23(2)

Опубликована: Май 26, 2023

Язык: Английский

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Biomaterial-assisted targeted and controlled delivery of CRISPR/Cas9 for precise gene editing

Biomaterials Science, Год журнала: 2023, Номер 11(11), С. 3762 - 3783

Опубликована: Янв. 1, 2023

Development of a novel biomaterial-based CRISPR-Cas9 system for the precise control genome editing.

Язык: Английский

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