Abstract
Gold
nanoparticles
(AuNPs)
are
promising
materials
for
many
bioapplications.
However,
upon
contacting
with
biological
media,
AuNPs
undergo
changes.
The
interaction
proteins
results
in
the
so‐called
protein
corona
(PC)
around
AuNPs,
leading
to
new
bioidentity
and
optical
properties.
Understanding
mechanisms
of
PC
formation
its
functions
can
help
us
utilise
benefits
avoid
drawbacks.
To
date,
most
previous
works
aimed
understand
governing
focused
on
spherical
nanoparticles,
although
non‐spherical
designed
a
wide
range
applications
biosensing.
In
this
work,
we
investigated
differences
anisotropic
(nanostars
particular)
from
joint
experimental
(extinction
spectroscopy,
zeta
potential
surface‐enhanced
Raman
scattering
[SERS])
computational
methods
(the
finite
element
method
molecular
dynamics
[MD]
simulations).
We
discovered
that
does
not
fully
cover
surface
leaving
SERS
hot‐spots
at
tips
high
curvature
edges
‘available’
analyte
binding
(no
signal
after
pre‐incubation
protein)
while
providing
protein‐induced
stabilization
(indicated
by
extinction
spectroscopy)
layer
particle's
core.
findings
confirmed
our
MD
simulations,
adsorption
energy
significantly
decreases
increased
radius
curvature,
so
(adsorption
energy:
2762.334
kJ/mol)
would
be
least
preferential
site
compared
core
11819.263
kJ/mol).
These
observations
will
development
nanostructures
improved
sensing
targeting
ability.
Advanced Materials,
Год журнала:
2023,
Номер
36(13)
Опубликована: Июль 12, 2023
Abstract
Clustered
regularly
interspaced
short
palindromic
repeats/associated
protein
9
(CRISPR/Cas9)
gene‐editing
technology
shows
promise
for
manipulating
single
or
multiple
tumor‐associated
genes
and
engineering
immune
cells
to
treat
cancers.
Currently,
most
strategies
rely
on
viral
delivery;
yet,
while
being
efficient,
many
limitations,
mainly
from
safety
packaging
capacity
considerations,
hinder
the
use
of
CRISPR
vectors
in
cancer
therapy.
In
contrast,
recent
advances
non‐viral
CRISPR/Cas9
nanoformulations
have
paved
way
better
gene
editing,
as
these
can
be
engineered
improve
safety,
efficiency,
specificity
through
optimizing
capacity,
pharmacokinetics,
targetability.
this
review,
advance
delivery
is
highlighted,
there
a
discussion
how
approaches
potentially
used
cancers
addressing
aforementioned
followed
by
perspectives
designing
proper
CRISPR/Cas9‐based
nanomedicine
system
with
translational
potential.
Abstract
Gold
nanoparticles
(AuNPs)
are
promising
materials
for
many
bioapplications.
However,
upon
contacting
with
biological
media,
AuNPs
undergo
changes.
The
interaction
proteins
results
in
the
so‐called
protein
corona
(PC)
around
AuNPs,
leading
to
new
bioidentity
and
optical
properties.
Understanding
mechanisms
of
PC
formation
its
functions
can
help
us
utilise
benefits
avoid
drawbacks.
To
date,
most
previous
works
aimed
understand
governing
focused
on
spherical
nanoparticles,
although
non‐spherical
designed
a
wide
range
applications
biosensing.
In
this
work,
we
investigated
differences
anisotropic
(nanostars
particular)
from
joint
experimental
(extinction
spectroscopy,
zeta
potential
surface‐enhanced
Raman
scattering
[SERS])
computational
methods
(the
finite
element
method
molecular
dynamics
[MD]
simulations).
We
discovered
that
does
not
fully
cover
surface
leaving
SERS
hot‐spots
at
tips
high
curvature
edges
‘available’
analyte
binding
(no
signal
after
pre‐incubation
protein)
while
providing
protein‐induced
stabilization
(indicated
by
extinction
spectroscopy)
layer
particle's
core.
findings
confirmed
our
MD
simulations,
adsorption
energy
significantly
decreases
increased
radius
curvature,
so
(adsorption
energy:
2762.334
kJ/mol)
would
be
least
preferential
site
compared
core
11819.263
kJ/mol).
These
observations
will
development
nanostructures
improved
sensing
targeting
ability.
