Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: May 20, 2024
Abstract
The
membrane-fusion-based
internalization
without
lysosomal
entrapment
is
advantageous
for
intracellular
delivery
over
endocytosis.
However,
protein
corona
formed
on
the
membrane-fusogenic
liposome
surface
converts
its
membrane-fusion
performance
to
lysosome-dependent
endocytosis,
causing
poorer
efficiency
in
biological
conditions.
Herein,
we
develop
an
antifouling
effective
vivo.
Leveraging
specific
lipid
composition
at
optimized
ratio,
such
facilitates
fusion
capacity
even
protein-rich
conditions,
attributed
copious
zwitterionic
phosphorylcholine
groups
protein-adsorption
resistance.
Consequently,
demonstrates
robust
membrane-fusion-mediated
medium
with
up
38%
fetal
bovine
serum,
outclassing
two
traditional
liposomes
4%
and
6%
concentrations.
When
injected
into
mice,
can
keep
their
membrane-fusion-transportation
behaviors,
thereby
achieving
efficient
luciferase
transfection
enhancing
gene-editing-mediated
viral
inhibition.
This
study
provides
a
promising
tool
under
complex
physiological
environments,
enlightening
future
nanomedicine
design.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
36(13)
Published: July 12, 2023
Abstract
Clustered
regularly
interspaced
short
palindromic
repeats/associated
protein
9
(CRISPR/Cas9)
gene‐editing
technology
shows
promise
for
manipulating
single
or
multiple
tumor‐associated
genes
and
engineering
immune
cells
to
treat
cancers.
Currently,
most
strategies
rely
on
viral
delivery;
yet,
while
being
efficient,
many
limitations,
mainly
from
safety
packaging
capacity
considerations,
hinder
the
use
of
CRISPR
vectors
in
cancer
therapy.
In
contrast,
recent
advances
non‐viral
CRISPR/Cas9
nanoformulations
have
paved
way
better
gene
editing,
as
these
can
be
engineered
improve
safety,
efficiency,
specificity
through
optimizing
capacity,
pharmacokinetics,
targetability.
this
review,
advance
delivery
is
highlighted,
there
a
discussion
how
approaches
potentially
used
cancers
addressing
aforementioned
followed
by
perspectives
designing
proper
CRISPR/Cas9‐based
nanomedicine
system
with
translational
potential.
Aggregate,
Journal Year:
2023,
Volume and Issue:
4(4)
Published: Feb. 22, 2023
Abstract
Gold
nanoparticles
(AuNPs)
are
promising
materials
for
many
bioapplications.
However,
upon
contacting
with
biological
media,
AuNPs
undergo
changes.
The
interaction
proteins
results
in
the
so‐called
protein
corona
(PC)
around
AuNPs,
leading
to
new
bioidentity
and
optical
properties.
Understanding
mechanisms
of
PC
formation
its
functions
can
help
us
utilise
benefits
avoid
drawbacks.
To
date,
most
previous
works
aimed
understand
governing
focused
on
spherical
nanoparticles,
although
non‐spherical
designed
a
wide
range
applications
biosensing.
In
this
work,
we
investigated
differences
anisotropic
(nanostars
particular)
from
joint
experimental
(extinction
spectroscopy,
zeta
potential
surface‐enhanced
Raman
scattering
[SERS])
computational
methods
(the
finite
element
method
molecular
dynamics
[MD]
simulations).
We
discovered
that
does
not
fully
cover
surface
leaving
SERS
hot‐spots
at
tips
high
curvature
edges
‘available’
analyte
binding
(no
signal
after
pre‐incubation
protein)
while
providing
protein‐induced
stabilization
(indicated
by
extinction
spectroscopy)
layer
particle's
core.
findings
confirmed
our
MD
simulations,
adsorption
energy
significantly
decreases
increased
radius
curvature,
so
(adsorption
energy:
2762.334
kJ/mol)
would
be
least
preferential
site
compared
core
11819.263
kJ/mol).
These
observations
will
development
nanostructures
improved
sensing
targeting
ability.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: May 20, 2024
Abstract
The
membrane-fusion-based
internalization
without
lysosomal
entrapment
is
advantageous
for
intracellular
delivery
over
endocytosis.
However,
protein
corona
formed
on
the
membrane-fusogenic
liposome
surface
converts
its
membrane-fusion
performance
to
lysosome-dependent
endocytosis,
causing
poorer
efficiency
in
biological
conditions.
Herein,
we
develop
an
antifouling
effective
vivo.
Leveraging
specific
lipid
composition
at
optimized
ratio,
such
facilitates
fusion
capacity
even
protein-rich
conditions,
attributed
copious
zwitterionic
phosphorylcholine
groups
protein-adsorption
resistance.
Consequently,
demonstrates
robust
membrane-fusion-mediated
medium
with
up
38%
fetal
bovine
serum,
outclassing
two
traditional
liposomes
4%
and
6%
concentrations.
When
injected
into
mice,
can
keep
their
membrane-fusion-transportation
behaviors,
thereby
achieving
efficient
luciferase
transfection
enhancing
gene-editing-mediated
viral
inhibition.
This
study
provides
a
promising
tool
under
complex
physiological
environments,
enlightening
future
nanomedicine
design.
