The Role of Piezo1 in Regulating Collagen Expression in Orbital Fibroblasts under High Pressure DOI
Yan Wen,

Taixing Liu,

Qingqing Tang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 10, 2024

Abstract Purpose.To explore the effect of Pizeo 1 on expression collagen Ⅰ\Ⅲ\Ⅴof orbital fibroblasts in pressure culture simulated high thyroid-associated ophthalmopathy (TAO). Methods.The primary guinea pig were isolated and cultured by enzymatic digestion method, levels Piezo1, α-SMA, collagenⅠ\Ⅲ\Ⅴin cells under different (0, 1, 2, 3Kpa) checked WB PCR. And then a constant 3KPa treated with concentrations (1, 5, 10 μmol/L) Piezo1 inhibitor GsMTx4, respectively. The collagenⅠ\Ⅲ\Ⅴ observed. CCK8 Flow cytometry used to observe cell proliferation apoptosis. Results.With increase pressure, protein mRNA increased significantly (P< 0.05). Meanwhile, inhibition GsMTx4 reduced OFs transdifferentiation Furthermore, was enhanced pressure. Under co-culture low concentration group promoted fibroblasts, (10μM) at early stage, but less than 5μM group. later inhibited. Compression alone or compression combined did not affect apoptosis fibroblasts. Conclusion. Down-regulating piezo1 can inhibit synthesis secretion collagenⅠ\Ⅲ\Ⅴ, which provide new idea for exploring fibrosis mechanism TAO.

Язык: Английский

Emerging Trends in Nanomedicine: Carbon-Based Nanomaterials for Healthcare DOI Creative Commons
Nargish Parvin, Vineet Kumar, Sang Woo Joo

и другие.

Nanomaterials, Год журнала: 2024, Номер 14(13), С. 1085 - 1085

Опубликована: Июнь 25, 2024

Carbon-based nanomaterials, such as carbon quantum dots (CQDs) and 2D nanosheets (graphene, graphene oxide, graphdiyne), have shown remarkable potential in various biological applications. CQDs offer tunable photoluminescence excellent biocompatibility, making them suitable for bioimaging, drug delivery, biosensing, photodynamic therapy. Additionally, CQDs' unique properties enable bioimaging-guided therapy targeted imaging of biomolecules. On the other hand, exhibit exceptional physicochemical attributes, with excelling biosensing also delivery antimicrobial applications, graphdiyne tissue engineering. Their properties, porosity high surface area, contribute to controlled release enhanced regeneration. However, challenges, including long-term biocompatibility large-scale synthesis, necessitate further research. Potential future directions encompass theranostics, immunomodulation, neural interfaces, bioelectronic medicine, expanding bioimaging capabilities. In summary, both hold promise revolutionize biomedical sciences, offering innovative solutions improved therapies diverse contexts. Addressing current challenges will unlock their full can shape medicine biotechnology.

Язык: Английский

Процитировано

14
Rectifying artificial nanochannels with multiple interconvertible permeability states DOI Creative Commons
Ruo‐Can Qian,

Man‐Sha Wu,

Zhenglin Yang

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 6, 2024

Abstract Transmembrane channels play a vital role in regulating the permeation process, and have inspired recent development of biomimetic channels. Herein, we report class artificial nanochannels based on DNAzyme-functionalized glass nanopipettes to realize delicate control channel permeability, whereby surface wettability charge can be tuned by metal ions DNAzyme-substrates, allowing reversible conversion between different permeability states. We demonstrate that reversibly switched four states showing distinct various functional molecules. By embedding into plasma membrane single living cells, achieve selective transport dye molecules across cell membrane. Finally, advanced functions including gene silencing miR-21 cancer cells Ca 2+ PC-12 cells. In this work, provide versatile tool for design rectifying with on-demand functions.

Язык: Английский

Процитировано

8
Protein-mediated fluorescent probes for bioimaging and biosensing: From fundamentals to applications DOI Open Access

Xiaokan Yu,

Chenming Li, Baozhu Wang

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 170, С. 117462 - 117462

Опубликована: Ноя. 23, 2023

Язык: Английский

Процитировано

11
A triphenylamine based fluorescent probe for Zn2+ detection and its applicability in live cell imaging DOI
Ling-hui Tang, Dong Yan,

Mingxue Cui

и другие.

Polyhedron, Год журнала: 2023, Номер 248, С. 116759 - 116759

Опубликована: Дек. 1, 2023

Язык: Английский

Процитировано

10
Wireless Stimulation of Barium Titanate@PEDOT Nanoparticles Toward Bioelectrical Modulation in Cancer DOI

Catarina Franco Jones,

Marta S. Carvalho, Akhil Jain

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Cancer cells possess distinct bioelectrical properties, yet therapies leveraging these characteristics remain underexplored. Herein, we introduce an innovative nanobioelectronic system combining a piezoelectric barium titanate nanoparticle core with conducting poly(3,4-ethylenedioxythiophene) shell (BTO@PEDOT NPs), designed to modulate cancer cell bioelectricity through noninvasive, wireless stimulation. Our hypothesis is that acting as nanoantennas, BTO@PEDOT NPs convert mechanical inputs provided by ultrasound (US) into electrical signals, capable of interfering the bioelectronic circuitry two human breast lines, MCF-7 and MDA-MB-231. Upon US stimulation, viability MDA-MB-231 treated 200 μg mL-1 reduced significantly 31% 24%, respectively, while healthy mammary fibroblasts (HMF) were unaffected treatment. Subsequent assays shed light on how this approach could interact cell's mechanisms, namely, increasing intracellular reactive oxygen species (ROS) calcium concentrations. Furthermore, was able polarize membranes, halting their cycle potentially harnessing tumorigenic characteristics. These findings underscore crucial role in progression highlight potential systems emerging promising strategy for intervention.

Язык: Английский

Процитировано

0
Understanding the Mechanism of Cardiotoxicity Induced by Nanomaterials: A Comprehensive Review DOI Creative Commons
Zaiyong Zheng, Shuang Zhu, Xiaobo Wang

и другие.

Small Science, Год журнала: 2025, Номер 5(5)

Опубликована: Фев. 20, 2025

Nanomaterials have been vastly used in daily life. However, owing to their unique properties, nanomaterials also show potential side effects. Among the various organs affected by nanomaterials, circulatory system stands out as particularly vulnerable, drawing additional attention its cardiac toxicity. To address cardiovascular nanotoxicity and further promote safe use of nanotechnology, a comprehensive review cardiotoxicity induced is provided. The begins identifying current research trends hotspots this field via bibliometric analysis. Subsequently, based on objectively obtained hotspots, mechanism nanotoxicity, including exposure route, membrane injury, ion disturbance, oxidative stress, inflammation, cell death, reviewed discussed. Finally, strategies for mitigation are proposed. objective assist readers understanding facilitate application human health.

Язык: Английский

Процитировано

0
Stimuli-Responsive PEGylated Nanocarriers DOI
Samah Hamed Almurisi, Praveen Rao, Thiagarajan Madheswaran

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Silk fibroin protein-templated gold nanoclusters for in vivo fluorescence imaging DOI

Shanshan He,

Baozhu Wang,

Harvey J. M. Hou

и другие.

Photochemical & Photobiological Sciences, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Язык: Английский

Процитировано

0
Wireless discharge of piezoelectric nanogenerator opens voltage-gated ion channels for calcium overload-mediated tumor treatment DOI
Yuchu He, Xiaoyu Yang, Meng Yuan

и другие.

Biomaterials, Год журнала: 2025, Номер unknown, С. 123311 - 123311

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Nanobionics and Nanomaterials: Impact on Soil Microbial Community and Functions DOI
Viabhav Kumar Upadhayay, Dhruv Mishra,

Manoj Kumar Prajapati

и другие.

Nanotechnology in the life sciences, Год журнала: 2025, Номер unknown, С. 37 - 69

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0