Extracellular vesicles in seminal fluid and effects on male reproduction. An overview in farm animals and pets

Animal Reproduction Science, Год журнала: 2021, Номер 246, С. 106853 - 106853

Опубликована: Сен. 16, 2021

Язык: Английский

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Microenvironment for spermatogenesis and sperm maturation

Histochemistry and Cell Biology, Год журнала: 2022, Номер 157(3), С. 273 - 285

Опубликована: Март 1, 2022

Язык: Английский

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Reproductive physiology of the boar: What defines the potential fertility of an ejaculate?

Animal Reproduction Science, Год журнала: 2024, Номер 269, С. 107476 - 107476

Опубликована: Апрель 18, 2024

Despite decades of research and handling semen for use in artificial insemination (AI) other assisted reproductive technologies, 5-10% selected boar sires are still considered sub-fertile, escaping current assessment methods sperm quality resilience to preservation. As end-product, the ejaculate (emitted spermatozoa sequentially exposed composite seminal plasma, SP) ought define homeostasis testes, epididymis, accessory sexual glands. Yet, linking findings production biology fertility is suboptimal. The present essay critically reviews how a fertile can help us diagnose both health handling, focusing on -available under development- identify suitable biomarkers cryotolerance fertility. Bulk SP, proteins microRNAs (miRNAs) have, albeit linked function after AI, failed enhance outcomes at commercial level, perhaps just being components complex functional pathway. Hence, focus now interaction sperm-SP, comparing vivo with ex vivo, regarding nano-sized lipid bilayer extracellular vesicles (sEVs) as priority. sEVs transport fragile molecules (lipids, proteins, nucleic acids) which, shielded from degradation, mediate cell-to-cell communication female internal genital tract. Such modulates essential processes, immunological tolerance. be harvested, characterized, stored, manipulated, e.g. used andrological diagnosis, selection breeders, alternatively additives improve cryosurvival

Язык: Английский

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Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(47)

Опубликована: Ноя. 11, 2024

Forecasted increases in the prevalence and severity of extreme weather events accompanying changes climatic behavior pose potential risk to reproductive capacity humans animals ecological agricultural significance. While several studies have revealed that heat stress induced by challenges such as testicular insulation can elicit a marked negative effect on male system, particularly production spermatozoa, less is known about immediate impact function following subchronic whole-body exposure elevated ambient temperature. To address this knowledge gap, we exposed unrestrained mice conditions emulate wave (daily cycle 8 h at 35 °C followed 16 25 °C) for period 7 d. Neither testes or epididymides heat-exposed exhibited evidence gross histological change, similarly, spermatozoa males retained their functionality ability support embryonic development. However, embryos generated from experienced pronounced gene expression linked acceleration early embryo development, aberrant blastocyst hatching, increased fetal:placental weight ratio. Such were causally associated with an altered sperm small noncoding RNA (sncRNA) profile, these developmental phenotypes recapitulated microinjection wild-type sired control RNAs extracted spermatozoa. data highlight even relatively modest excursions temperature affect identify sncRNA profile particular point vulnerability imposed environmental stress.

Язык: Английский

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Extracellular vesicles in reproductive medicines

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 243 - 281

Опубликована: Янв. 1, 2025

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miRNA-Profiling in Ejaculated and Epididymal Pig Spermatozoa and Their Relation to Fertility after Artificial Insemination

Biology, Год журнала: 2022, Номер 11(2), С. 236 - 236

Опубликована: Фев. 1, 2022

MicroRNAs (miRNAs) are short non-coding RNAs (20-25 nucleotides in length) capable of regulating gene expression by binding -fully or partially- to the 3'-UTR target messenger RNA (mRNA). To date, several studies have investigated role sperm miRNAs spermatogenesis and their remaining presence toward fertilization early embryo development. However, little is known about miRNA cargo different sources possible implications boar fertility. Here, we characterized differential abundance spermatozoa from terminal segment epididymis three fractions pig ejaculate (sperm-peak, sperm-rich, post-sperm rich) comparing breeding boars with higher (HF) lower (LF) fertility after artificial insemination (AI) using high-output small sequencing. We identified five that, our knowledge, not been previously reported pigs (mir-10386, mir-10390, mir-6516, mir-9788-1, mir-9788-2). Additionally, four (mir-1285, mir-92a, mir-34c, mir-30), were differentially expressed among sourced cauda epididymis, also was found between HF LF groups mir-182, mir-1285, mir-191, mir-96. These genes key roles fertility, survival, immune tolerance, cell cycle regulation, others. Linking current findings specific proteins would help predict future AI-sires.

Язык: Английский

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Updating Research on Extracellular Vesicles of the Male Reproductive Tract in Farm Animals: A Systematic Review

Animals, Год журнала: 2024, Номер 14(21), С. 3135 - 3135

Опубликована: Окт. 31, 2024

This systematic review examined research studies on extracellular vesicles (EVs) of the male reproductive tract in livestock species to summarize topics and methodologies used, key findings, future directions. PubMed Scopus were searched for time ranges up 1 September 2024, 1383 articles identified. The application screening eligibility criteria resulted selection 79 focusing EVs livestock. Porcine bovine most studied. A variety EV isolation techniques with ultracentrifugation being common. Characterization was a weak point, only 24.05% characterizing according MISEV guidelines. Inadequate characterization compromises reliability results. results 19 that provided good showed from are phenotypically compositionally heterogeneous. These papers also these would be involved regulation sperm functionality. Research remains scarce, further is needed, which should include appropriate aim find efficient methods isolate them assess their involvement functionality spermatozoa cells female genital tract.

Язык: Английский

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Biogenic and risk elements in wild boar testes and relation to spermatozoa motility

Journal of Environmental Science and Health Part A, Год журнала: 2024, Номер unknown, С. 1 - 6

Опубликована: Ноя. 5, 2024

Environmental pollution results in serious health hazards to animals, reflected biogenic and risk element concentrations animal tissues. The objective of this study was examinate concentration selected elements testes, epididymal spermatozoa motility wild boars. Wild boars were hunted region Žuhračka - Levice, Slovak Republic. Testes removed postmortem, collected from cauda epididymis assessed by CASA system. Elements measured ICP CV-AAS. Spermatozoa 44.29% progressive 18.47%. Concentration testes following order: K > Na Mg Ca Fe Zn Al Cu Se Mn As Cr Pb Mo Sr Ni Ba Cd Li Hg. most notable correlations indicate association between total motility, as well with furthermore velocity curved line beat cross frequency. A high positive significant correlation found mercury data may serve a fine control indicator detect potentially toxic accumulated polluted environment that can affect reproduction animals.

Язык: Английский

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Improving the process of spermatogenesis in azoospermic mice using spermatogonial stem cells co-cultured with epididymosomes in three-dimensional culture system

Life Sciences, Год журнала: 2022, Номер 310, С. 121057 - 121057

Опубликована: Окт. 8, 2022

Язык: Английский

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Morphology of the epididymal duct of the domestic pig (Sus scrofa domesticus)

Journal of Morphology, Год журнала: 2024, Номер 285(2)

Опубликована: Янв. 18, 2024

Abstract The study provides a general overview of the morphology epididymal duct in pigs. Four epididymides from two sexually mature boars were dissected into 32 segments and examined histologically. Duct lumen wall thickness measured relative surface area different components was assessed by Chalkley's random hit method. epithelial lining characterized at X1000. Lumen diameter efferent ductules averaged 177 30 µm, respectively. Of caput to distal corpus luminal 332 with narrower section proximal corpus. Wall 70 µm. In cauda, increased 717 751 consists single layer columnar cells average height 21 ciliated, pseudo‐stratified epithelium composed “basal cells” “principal cells.” Particularly tall principal (96 µm) found caput. Height decreased 40 µm cauda. Microvilli 14−17 long but 5 folded more so Secretory granules (epididymosomes) present small amounts duct; largest quantities occurred Leukocytes throughout duct, albeit insignificant numbers. method showed rapid spermatozoan transport through large fluid. Sperm concentration due fluid resorption caput, highest flexure cauda caudal flexure, indicating secretory activity.

Язык: Английский

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