Cells,
Год журнала:
2022,
Номер
11(18), С. 2845 - 2845
Опубликована: Сен. 12, 2022
Preservation
of
human
spermatozoa
in
vitro
at
normothermia
or
hypothermia
maintaining
their
functions
and
fertility
for
several
days
plays
a
significant
role
reproductive
biology
medicine.
However,
it
is
well
known
that
left
deteriorate
over
time
irreversibly
as
the
consequence
various
stresses
such
change
osmolarity,
energy
deficiency,
oxidative
damage,
leading
to
substantial
limitations
including
need
semen
examinations,
preservation,
assisted
technology.
These
problems
may
be
addressed
with
aid
non-freezing
storage
techniques.
The
main
most
effective
preservation
strategies
are
partial
total
replacement
seminal
plasma
culture
medium,
named
extenders,
temperature-induced
metabolic
restriction.
Semen
extenders
consist
buffers,
osmolytes,
antioxidants,
etc.
protect
against
above-mentioned
adverse
factors.
Extended
has
negative
effect
on
sperm
parameters,
whereas
its
ART
outcomes
remains
inconsistent.
duration,
temperature,
pre-treatment
should
determined
according
aims
preservation.
Advanced
techniques
nanotechnology
omics
have
been
introduced
show
great
potential
lifespan
extension
sperm.
It
certain
more
patients
will
benefit
from
near
future.
This
review
provided
an
overview
current
knowledge
prospects
prolonged
vitro.
Journal of Extracellular Vesicles,
Год журнала:
2022,
Номер
11(10)
Опубликована: Окт. 1, 2022
Abstract
Extracellular
vesicles
(EVs)
have
been
shown
to
be
involved
in
cell‐cell
communication
and
take
part
both
physiological
pathological
processes.
Thanks
their
exclusive
cargo,
which
includes
proteins,
lipids,
nucleic
acids
from
the
originating
cells,
they
are
gaining
interest
as
potential
biomarkers
of
disease.
In
recent
years,
appealing
features
fascinating
researchers
all
over
world,
thus
increasing
number
vitro
studies
focused
on
EV
release,
content,
biological
activities.
Cultured
cell
lines
most‐used
source
EVs;
however,
EVs
released
cultures
influenced
by
culture
conditions,
such
use
foetal
bovine
serum
(FBS).
FBS
is
most
common
supplement
for
media,
but
it
also
a
contaminants,
exogenous
EVs,
RNA,
protein
aggregates,
that
can
contaminate
cell‐derived
influence
cargo
composition.
The
presence
contaminants
samples
well‐known
issue
limits
clinical
applications
need
standardization.
this
review,
we
will
discuss
pros
cons
using
well
protocols
used
remove
FBS.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 23, 2024
Abstract
Background
Porcine
seminal
plasma
(SP)
is
endowed
with
a
heterogeneous
population
of
extracellular
vesicles
(sEVs).
This
study
evaluated
the
immunophenotypic
profile
by
high-sensitivity
flow
cytometry
eight
sEV
subpopulations
isolated
according
to
their
size
(small
[S-sEVs]
and
large
[L-sEVs])
from
four
different
SP
sources,
namely
three
ejaculate
fractions
(the
first
10
mL
sperm
rich
fraction
[SRF-P1],
remaining
SRF
[SRF-P2],
post-SRF
[PSRF])
entire
(EE).
Methods
Seminal
EVs
were
using
exclusion
chromatography-based
protocol
six
pools
(five
ejaculates/pool)
each
source
characterized
complementary
approaches
including
total
protein
(BCA™assay),
particle
distribution
(dynamic
light
scattering),
morphology
(transmission
electron
microscopy),
purity
(albumin
Western
blot).
Expression
CD9,
CD63,
CD81,
CD44
HSP90β
was
analyzed
in
all
MIFlowCyt-EV
guidelines,
an
accurate
calibration,
controls,
discrimination
CFSE-labelling.
Results
Each
subpopulation
exhibited
specific
profile.
The
percentage
sEVs
positive
for
CD81
differed
between
S-
L-sEVs
(
P
<
0.0001).
Specifically,
CD9
CD63
higher
that
lower
than
sources.
However,
HSP90β-positive
S-sEVs
SRF-P1
EE
samples.
also
among
sources
0.0001),
being
highest
PSRF
Notably,
virtually
expressed
(range:
88.04–98.50%).
Conclusions
demonstrated
utility
immunophenotyping,
allowing
identification
distinct
may
have
cellular
origin,
cargo,
functions,
target
cells.
Theriogenology,
Год журнала:
2024,
Номер
219, С. 167 - 179
Опубликована: Фев. 26, 2024
Porcine
seminal
plasma
(SP)
is
loaded
with
a
heterogeneous
population
of
extracellular
vesicles
(sEVs)
that
modulate
several
reproductive-related
processes.
This
study
investigated
the
effect
two
sEV
subsets,
small
(S-sEVs)
and
large
(L-sEVs),
on
porcine
in
vitro
fertilization
(IVF).
The
sEVs
were
isolated
from
nine
SP
pools
(five
ejaculates/pool)
using
size-exclusion
chromatography-based
procedure
characterized
for
quantity
(total
protein),
morphology
(cryogenic
electron
microscopy),
size
distribution
(dynamic
light
scattering),
purity
EV-protein
markers
(flow
cytometry;
albumin,
CD81,
HSP90β).
characterization
confirmed
existence
subsets
high
(low
albumin
content)
differed
(S-
L-sEVs).
In
was
performed
matured
oocytes
frozen-thawed
spermatozoa
IVF
medium
supplemented
during
gamete
coincubation
(1
h
at
38.5
°C,
5
%
CO2
humidified
atmosphere)
three
different
concentrations
each
subset:
0
(control,
without
sEVs),
0.1,
0.2
mg/mL.
first
experiment
showed
sEVs,
regardless
subset
concentration,
decreased
penetration
rates
total
efficiency
(P
<
0.0001).
subsequent
experiment,
it
shown
impaired
ability
to
bind
zona
pellucida
following
subset,
bound
sperm
but
not
oocytes,
indicating
would
affect
functionality
oocyte
functionality.
lack
by
incubating
prior
IVF,
achieving
sperm-zona
binding
results
similar
those
control.
last
conducted
under
conditions,
analyzed
terms
tyrosine
phosphorylation,
acrosome
integrity
metabolism.
did
phosphorylation
or
integrity,
influence
metabolism
decreasing
ATP
production
capacitating
conditions.
conclusion,
this
demonstrated
presence
impairs
outcomes,
most
likely
altering
Molecular & Cellular Proteomics,
Год журнала:
2023,
Номер
22(4), С. 100514 - 100514
Опубликована: Фев. 14, 2023
Seminal
plasma
contains
many
morphologically
heterogeneous
extracellular
vesicles
(sEVs).
These
are
sequentially
released
by
cells
of
the
testis,
epididymis,
and
accessory
sex
glands
involved
in
male
female
reproductive
processes.
This
study
aimed
to
define
depth
sEV
subsets
isolated
ultrafiltration
size
exclusion
chromatography,
decode
their
proteomic
profiles
using
liquid
chromatography-tandem
mass
spectrometry,
quantify
identified
proteins
sequential
window
acquisition
all
theoretical
spectra.
The
were
defined
as
large
(L-EVs)
or
small
(S-EVs)
protein
concentration,
morphology,
distribution,
EV-specific
markers
purity.
Liquid
spectrometry
a
total
1034
proteins,
737
them
quantified
SWATH
S-EVs,
L-EVs,
non-EVs-enriched
samples
(18-20
chromatography-eluted
fractions).
differential
expression
analysis
revealed
197
differentially
abundant
between
both
EV
subsets,
S-EVs
37
199
L-EVs
versus
samples,
respectively.
gene
ontology
enrichment
suggested,
based
on
type
detected,
that
could
be
mainly
through
an
apocrine
blebbing
pathway
modulating
immune
environment
tract
well
during
sperm-oocyte
interaction.
In
contrast,
fusion
multivesicular
bodies
with
membrane
becoming
sperm
physiological
processes,
such
capacitation
avoidance
oxidative
stress.
conclusion,
this
provides
procedure
capable
isolating
EVs
from
pig
seminal
high
degree
purity
shows
differences
profile
indicating
different
sources
biological
functions
for
sEVs.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(5), С. 4818 - 4818
Опубликована: Март 2, 2023
Seminal
plasma
contains
numerous
extracellular
vesicles
(sEVs).
Since
sEVs
are
apparently
involved
in
male
(in)fertility,
this
systematic
review
focused
on
studies
specifically
investigating
such
relationship.
Embase,
PubMed,
and
Scopus
databases
were
searched
up
to
31
December
2022,
primarily
identifying
a
total
of
1440
articles.
After
processing
for
screening
eligibility,
305
selected
as
they
sEVs,
42
them
considered
eligible
because
included
the
word
fertility
or
related
infertility,
subfertility,
fertilization,
recurrent
pregnancy
loss
title,
objective(s),
and/or
keywords.
Only
nine
met
inclusion
criteria,
namely
(a)
conducting
experiments
aimed
at
associating
with
concerns
(b)
isolating
adequately
characterizing
sEVs.
Six
conducted
humans,
two
laboratory
animals,
one
livestock.
The
highlighted
some
sEV
molecules,
proteins
small
non-coding
RNAs,
that
showed
differences
between
fertile
subfertile
infertile
males.
content
was
also
sperm
fertilizing
capacity,
embryo
development,
implantation.
Bioinformatic
analysis
revealed
several
fertility-related
would
be
cross-linked
each
other
biological
pathways
(i)
EV
release
loading
(ii)
membrane
organization.
Frontiers in Veterinary Science,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 25, 2024
Extracellular
vesicles
are
nanoscale
that
transport
signals
between
cells,
mediating
both
physiological
and
pathological
processes.
EVs
facilitate
conserved
intercellular
communication.
By
transferring
bioactive
molecules
coordinate
systemic
responses,
regulating
homeostasis,
immunity,
disease
progression.
Given
their
biological
importance
involvement
in
pathogenesis,
show
promise
as
biomarkers
for
veterinary
diagnosis,
candidates
vaccine
production,
treatment
agents.
Additionally,
different
or
engineering
methods
could
be
used
to
boost
the
capability
of
extracellular
vesicles.
Despite
emerging
interest,
EV
research
has
been
predominantly
human-based.
Critical
knowledge
gaps
remain
regarding
isolation
protocols,
cargo
loading
mechanisms,
vivo
biodistribution,
species-specific
functions.
Standardized
characterization
validation
lacking.
Regulatory
uncertainties
impede
clinical
translation.
Advances
fundamental
biology
technology
needed
propel
field
forward.
This
review
introduces
from
a
perspective
by
introducing
latest
studies,
highlighting
potential
while
analyzing
challenges
motivate
expanded
investigation
Animal Reproduction Science,
Год журнала:
2021,
Номер
246, С. 106884 - 106884
Опубликована: Ноя. 5, 2021
The
sperm
plasma
membrane
is
important
in
modulating
many
functions.
composed
of
a
complex
mixture
lipids
including
phospholipids,
glycolipids
and
sterols.
There
are
differences
composition
among
mammalian
species
with
two
groups
differing
the
most
abundant
polyunsaturated
fatty
acid
(PUFA),
either
docosahexaenoic
(ω-3
PUFA)
or
docosapentaenoic
(ω-6
PUFA).
During
testicular
epididymal
maturation,
evolves
spermatozoa
gaining
capacity
for
fertilization.
importance
metabolism
complete
spermatogenesis
has
been
elucidated
using
gene
knockout
mice.
transit
cholesterol
content
decreases
PUFA
increases,
conferring
more
fluidity
properties
to
membrane.
relatively
lesser
antioxidant
enzymes
larger
make
particularly
susceptible
lipid
peroxidation
during
preservation.
In
numerous
studies,
there
was
adding
antioxidants
diet
animals
semen
extenders
aim
improve
integrity.
This
review
highlights
current
knowledge
on
effects
function
domestic
animals.
