Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Окт. 11, 2022
Abstract
The
purpose
of
this
study
was
to
monitor
concentration
selected
biological
and
risk
elements
in
testes
later
epididymal
spermatozoa
motility
wild
boars
(Sus
scrofa
scrofa)
as
well
their
association.
Wild
were
hunted
region
Žuhračka
-
Levice
branch
plant,
Slovak
Republic.
Testes
surgically
removed
post-mortem
transported
the
laboratory.
Spermatozoa
obtained
by
dissecting
distal
portion
cauda
epididymis
analysed
Computer
assisted
semen
analyzer
(CASA)
system.
Concentration
measured
inductively-coupled
plasma
optical
emission
spectrometry
(ICP-OES)
cold
vapor
absorption
spectrometer
(MA-3
Solo
Mercury
Analyzer).
Total
at
level
44.29%
progressive
18.47%.
following
order:
K
>
Na
Mg
Ca
Fe
Zn
Al
Cu
Se
Mn
As
Cr
Pb
Mo
Sr
Ni
Ba
Cd
Li
Hg.
Negative
association
observed
between
motility,
further
velocity
curved
line
beat
cross
frequency.
showed
positive
correlation
with
Our
results
suggest
that
some
chemical
accumulated
from
polluted
environment
can
affect
reproduction
animals.
Biology of Reproduction,
Год журнала:
2022,
Номер
108(1), С. 52 - 71
Опубликована: Ноя. 2, 2022
Abstract
Bovine
male
fertility
in
animals
has
a
direct
impact
on
the
productivity
of
dairy
herds.
The
epididymal
sperm
maturations
involve
extensive
surface
modifications
to
gain
fertilizing
ability,
especially
by
absorptions
plethora
biomolecules,
including
glycoprotein
beta-defensins
(BDs),
enzymes,
organic
ions,
protein,
and
phospholipids.
Defensins
are
broad-range
nonspecific
antimicrobial
peptides
that
exhibit
strong
relations
with
innate
adaptive
immunity,
but
their
roles
relatively
recently
identified.
In
course
evolution,
BD
genes
give
rise
different
clusters
specific
functions,
reproductive
undergoing
duplications
nonsynonymous
mutations.
polymorphisms
have
been
reported
milk
compositions,
disease
resistance,
activities.
However,
recent
decades,
link
emerged
as
an
appealing
improvement
performance
such
motility,
membrane
integrity,
cervical
mucus
penetration,
evading
uterus
immunosurveillance,
oviduct
cell
attachment,
egg
recognition.
reproductive-specific
glycosylated
class-A
BDs
(CA-BDs)
shown
age-
sex-specific
expressions
organs,
signifying
physiological
pleiotropism,
maturation
transport
female
tract.
By
considering
adult
organ-specific
expressions,
importance
functionalities,
bioinformatic
analysis,
we
selected
two
bovine
BBD126
BBD129
novel
potential
biomarkers
fertility.
Despite
BDs,
however,
genomic
characterization
most
across
livestock
nonmodel
organisms
remains
predictive/incomplete.
current
review
discusses
our
understanding
pleiotropic
polymorphism,
structural
attributes
concerning
fertilizability
gamete
animals.
Scientific Reports,
Год журнала:
2022,
Номер
12(1)
Опубликована: Дек. 17, 2022
Abstract
This
single
cell
study
aimed
to
clarify
whether
an
elevated
incidence
of
sperm
with
a
retained
cytoplasmic
droplet
(CD)
can
be
compensated
by
higher
number
in
boar
semen
doses
maintain
fertility.
Cluster
analysis
motile
spermatozoa
(ten
boars)
revealed
that
CD
are
underrepresented
the
fast,
linearly
moving
cohort
compared
morphologically
normal
sperm.
Nonetheless,
response
motility
stimulator
procaine
was
barely
affected
distal
(Cramer’s
V
=
0.14),
but
moderately
proximal
(V
0.28).
Viability
lower
(
p
<
0.05)
not
during
168
h
storage
extended
samples
(n
11)
and
subsequent
thermic
stress.
Morphologically
from
normospermic
10)
or
high
(≥
15%)
9)
had
similar
patterns
responses
procaine.
The
origin
no
effect
on
viability
>
0.05;
n
26).
In
conclusion,
enhanced
prevalence
seems
compensable
increase
numbers
doses.
Viruses,
Год журнала:
2021,
Номер
14(1), С. 31 - 31
Опубликована: Дек. 24, 2021
African
swine
fever
(ASF)
has
evolved
from
an
exotic
animal
disease
to
a
threat
global
pig
production.
An
important
avenue
for
the
wide-spread
transmission
of
diseases
is
their
dissemination
through
boar
semen
used
artificial
insemination.
In
this
context,
we
investigated
role
male
reproductive
organs
in
ASF.
Mature
domestic
boars
and
adolescent
wild
boars,
inoculated
with
different
ASF
virus
strains,
were
by
means
virological
pathological
methods.
Additionally,
electron
microscopy
was
employed
investigate
vitro
sperm.
The
viral
genome,
antigens
infectious
could
be
found
all
gonadal
tissues
accessory
sex
glands.
antigen
mRNAs
mainly
mononuclear
cells
respective
tissues.
However,
some
other
cell
types,
including
Leydig,
endothelial
stromal
cells,
also
positive.
Using
RNAScope,
p72
mRNA
scattered
halo
epididymal
duct
epithelium,
which
point
disruption
barrier.
No
direct
infection
spermatozoa
observed
immunohistochemistry,
or
microscopy.
Taken
together,
our
results
strengthen
assumption
that
ASFV
can
transmitted
via
semen.
Future
studies
are
needed
explore
excretion
dynamics
efficiency.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(18), С. 13750 - 13750
Опубликована: Сен. 6, 2023
The
main
cation/calcium
channel
of
spermatozoa
(CatSper),
first
identified
in
2001,
has
been
thoroughly
studied
to
elucidate
its
composition
and
function,
while
distribution
among
species
sperm
sources
is
yet
incomplete.
CatSper
composed
several
subunits
that
build
a
pore-forming
calcium
channel,
mainly
activated
vivo
ejaculated
cells
by
intracellular
alkalinization
progesterone,
as
suggested
the
vitro
examinations.
relevance
dual:
maintain
homeostasis
(alongside
plethora
membrane
channels
present)
well
being
involved
pre-fertilization
events,
such
capacitation,
hyperactivation
motility
acrosome
reaction,
with
remarkable
differences.
Interestingly,
observed
variations
localization
plasma
seem
depend
on
source
explored
(i.e.,
epididymal
or
ejaculated,
immature
mature,
processed
not),
method
used
for
examination
and,
particularly,
specificity
antibodies
employed.
In
addition,
despite
multiple
findings
showing
fertilization,
few
studies
have
biomarker
fine-tune
diagnosis
sub-fertility
livestock
even
consider
potential
control
fertilization
plague
animals,
more
ethically
defensible
strategy
than
implicating
pharmacologically
modify
male-related
fertility
humans,
pets
wild
animals.
This
review
describes
inter-
intra-species
differences
localization,
structure
function
calling
caution
when
considering
manipulation
improvement.
African
swine
fever
(ASF)
has
evolved
from
an
exotic
animal
disease
to
a
threat
global
pig
production.
An
important
avenue
for
wide-spread
transmission
of
diseases
is
the
dissemination
viruses
through
boar
semen
used
artificial
insemination
(AI).
In
this
context,
we
investigated
role
male
reproductive
organs
in
ASF.
Mature
domestic
boars
and
adolescent
wild
inoculated
with
different
ASF
virus
strains
were
by
means
virological
pathological
methods.
Additionally,
electron
microscopy
was
employed
investigate
vitro
sperm.
Viral
genome,
antigen
infectious
could
be
found
all
gonadal
tissues
accessory
sex
glands.
The
viral
mRNAs
mainly
mononuclear
cells
respective
tissues.
However,
some
other
cell
types,
including
Leydig,
endothelial
stromal
also
positive.
Using
RNAScope,
p72
mRNA
scattered
halo
epididymal
duct
epithelium
which
point
disruption
barrier.
No
direct
infection
spermatozoa
observed
immunohistochemistry
or
microscopy.
Taken
together,
our
results
strengthen
assumption
that
ASFV
can
transmitted
via
semen.
Future
studies
are
needed
explore
excretion
dynamics
efficiency.
Abstract
Background
The
endoplasmic
reticulum
(ER)
is
the
central
hub
for
protein
quality
control,
where
disulfide
isomerases
(PDIs),
encoded
by
at
least
21
genes,
play
a
pivotal
role.
These
multifunctional
proteins
contribute
to
bond
formation,
proper
folding,
and
modifications,
may
act
as
hormone‐binding
(e.g.,
steroids),
influencing
hormone
biology.
interplay
between
ER
proteostasis,
PDIs,
epididymis—a
crucial
site
sperm
maturation—remains
largely
understudied.
Objectives
This
study
characterizes
transcriptional
signatures
of
Pdi
genes
in
epididymis.
Material
methods
Transcriptional
profiles
selected
were
assessed
adult
Wistar
rat
tissues,
epididymis
under
different
experimental
conditions
(developmental
stages,
surgical
castration,
efferent
ductules
ligation
[EDL]).
In
silico
bioinformatic
analyses
identified
expression
trends
this
gene
family
human
epididymal
segments.
Results
P4hb
,
Pdia3
Pdia5
Pdia6
Erp44
Erp29
Casq1
transcripts
detected
both
reproductive
non‐reproductive
while
Casq2
exhibited
higher
abundance
vas
deferens,
prostate,
heart.
Pdilt
highly
expressed
testis,
Pdia2
heart,
showed
minimal
mRNA
levels
mesonephric
duct,
embryonic
precursor,
mRNAs
found
gestational
day
(GD)
17.5.
Except
which
remained
stable,
these
transcript
increased
from
GD17.5
GD20.5,
when
morphogenesis
occurs,
maintained
varying
degrees
during
postnatal
development.
Surgical
castration
downregulated
transcripts,
an
effect
reversed
testosterone
replacement.
Conversely,
unaffected
EDL,
except
was
reduced
caput
All
PDI
diverse
across
Discussion
conclusion
findings
lay
foundations
explore
As
considerable
proportion
male
infertility
cases
are
idiopathic,
targeting
hormonal
regulation
control
represents
route
address
advance
therapeutic
interventions
domain.
Jurnal Medik Veteriner,
Год журнала:
2024,
Номер
7(2), С. 300 - 309
Опубликована: Окт. 4, 2024
The
epididymis
is
a
crucial
component
of
the
goat's
reproductive
structure.
responsible
for
transportation,
concentration,
and
maturation
sperm.
This
study
aimed
to
examine
differences
in
structure
macro
microanatomy,
tissue
composition,
existence
androgen
receptors
Kacang
goats
aged
4,
8,
12
months.
assessment
macroscopic
organ
growth
was
done
immediately
after
sampling,
whereas
microscopic
measurements
were
carried
out
following
histological
preparations
using
hematoxylin-eosin
(HE)
immunohistochemical
(IHC)
procedures.
results
on
anatomy
indicated
significant
association
between
age
with
width
caput
dexter
sinister
circumference
right
cauda.
However,
no
relationship
found
corpus
length
left
cauda's
circumference.
Significant
variations
observed
diameter
concentration
agglutinated
spermatozoa
lumen
during
histomorphometry
three
groups
goats.
There
statistically
expression
among
groups.
showed
that
correlation
coefficient
test
reveals
positive
dimensions,
indicating
these
tend
grow
as
increases.
On
other
hand,
epididymal
exhibits
ages
4
months,
8
months
suggesting
sperm
becomes
fully
matured
by
Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Окт. 11, 2022
Abstract
The
purpose
of
this
study
was
to
monitor
concentration
selected
biological
and
risk
elements
in
testes
later
epididymal
spermatozoa
motility
wild
boars
(Sus
scrofa
scrofa)
as
well
their
association.
Wild
were
hunted
region
Žuhračka
-
Levice
branch
plant,
Slovak
Republic.
Testes
surgically
removed
post-mortem
transported
the
laboratory.
Spermatozoa
obtained
by
dissecting
distal
portion
cauda
epididymis
analysed
Computer
assisted
semen
analyzer
(CASA)
system.
Concentration
measured
inductively-coupled
plasma
optical
emission
spectrometry
(ICP-OES)
cold
vapor
absorption
spectrometer
(MA-3
Solo
Mercury
Analyzer).
Total
at
level
44.29%
progressive
18.47%.
following
order:
K
>
Na
Mg
Ca
Fe
Zn
Al
Cu
Se
Mn
As
Cr
Pb
Mo
Sr
Ni
Ba
Cd
Li
Hg.
Negative
association
observed
between
motility,
further
velocity
curved
line
beat
cross
frequency.
showed
positive
correlation
with
Our
results
suggest
that
some
chemical
accumulated
from
polluted
environment
can
affect
reproduction
animals.
