Inactivation Protocols for African Swine Fever Virus in Serum and Saliva Samples DOI Open Access
Lorena Franco‐Martínez, Martin Beer, Silvia Martı́nez-Subiela

и другие.

Опубликована: Май 30, 2022

African swine fever (ASF) is a notifiable viral disease of domestic and wild suids. Despite intensive re-search efforts, the pathogenesis still far from being understood. Analysis biomarkers in different body fluids may supplement traditional studies. As reliable protocols are often es-tablished laboratories with lower biosafety, inactivation samples crucial. The objective this study was to find procedure that inactivates virus while preserving for down-stream analyses. To means, three were employed, namely Tergitol-type NP-40 (NP-40) polyoxyethylene-p-t-octylphenol (Triton X-100), respectively, one 95 °C heating. It could be demonstrated all treated 0.5% (v/v) concentration both deter-gents showed absence infectivity. same true heated samples. However, serum not suitable Next, treatment impact on biomarker readouts assessed. While had an detection biomarkers, correlation retained. Especially desired detergent more accurate measurements achieving efficient inactivation. Based these studies, can reliably inactivated most analyses thus broader interdisciplinary cooperation possible.

Язык: Английский

Pathology of African Swine Fever in Wild Boar Carcasses Naturally Infected with German Virus Variants DOI Creative Commons
Julia Sehl‐Ewert, Paul Deutschmann, Angele Breithaupt

и другие.

Pathogens, Год журнала: 2022, Номер 11(11), С. 1386 - 1386

Опубликована: Ноя. 20, 2022

In 2020, African swine fever (ASF) was first identified in German wild boar, reaching a case number of about 4400 to date. Upon experimental infection, pathology is well documented; however, data on field infections are scarce domestic pigs and not available from respectively. Although the ASF viral genome considered exceptionally stable, total five lineages with 10 distinct virus variants genotype II have emerged Eastern Germany. To investigate naturally infected boar evaluate II, III IV for their virulence, carcasses were obtained three different outbreak areas. The underwent virological pathomorphological investigation. animals revealed characteristic lesions highest severity accompanied by bacterial several cases. particular, variant Spree-Neiße (SN) district showed lower loads antigen scores, but simultaneously tended reveal more chronic lesions. Our findings indicate protracted course disease at least after infection IV, need confirmation under standardized conditions. There strong monitor differences virulence among identify potential attenuation that might complicate diagnosis. addition, veterinarians, hunters farmers be made aware less acute courses consider this as an important differential classical fever.

Язык: Английский

Процитировано

10
Artificial Insemination as an Alternative Transmission Route for African Swine Fever Virus DOI Creative Commons
Virginia Friedrichs,

Darwin Reicks,

Tobias Hasenfuß

и другие.

Pathogens, Год журнала: 2022, Номер 11(12), С. 1539 - 1539

Опубликована: Дек. 14, 2022

The rapid spread of the African swine fever virus (ASFV), causing severe disease with often high fatality rates in Eurasian suids, prevails as a threat for pig populations and dependent industries worldwide. Although advancing scientific progress continually enhances our understanding ASFV pathogenesis, alternative transmission routes have yet to be assessed. Here, we demonstrate that can efficiently transferred from infected boars naïve recipient gilts through artificial insemination (AI). In modern production, semen boar studs supplies many sow herds. Thus, infection stud presents risk rapidly widely distributing within or between countries. Daily blood collection four after intramuscular inoculation strain ‘Estonia 2014’ resulted detection genomes early 2 dpi, at 1 dpi while quality remained largely unaffected. Ultimately, extended semen, 7 14 were positive by days post insemination, all 35 insemination. Twelve out 13 pregnant aborted resorbed onset fever. A proportion fetuses originating remaining gilt showed both abnormalities replication fetal tissues. present evidence efficient via AI also implanted embryos. These results underline critical role could play transmission.

Язык: Английский

Процитировано

10
Artificial insemination as alternative transmission route for African Swine Fever Virus DOI Open Access
Virginia Friedrichs,

Darwin Reicks,

Tobias Hasenfuss

и другие.

Опубликована: Ноя. 22, 2022

Rapid spread of African swine fever virus (ASFV), causing severe disease with often high fatality rates in Eurasian suids, prevails as a threat for pig populations and dependent industries world-wide. Although advancing scientific progress continually enhances our understanding ASFV pathogenesis, alternative transmission routes have yet to be assessed. Here, we demonstrate that can efficiently transferred from infected boars naïve recipient gilts through artificial insemination (AI). In modern production, semen boar studs supplies many sow herds. Thus, the infection stud presents risk rapidly widely distributing within or between countries. Daily blood collection four after intramuscular inoculation strain ‘Estonia 2014’ resulted detection genomes early 2 dpi, at 1 dpi while quality remained largely unaffected. Ultimately, extended semen, 7 14 were positive by days post insemination, all 35 insemi-nation. Twelve out 13 aborted onset fever. A proportion fetuses originating remaining gilt showed both abnormalities replication fetal tissues. present evidence efficient via AI also im-planted embryos. These results underline critical role could play transmission.

Язык: Английский

Процитировано

6
Pathology of African Swine Fever in Reproductive Organs of Mature Breeding Boars DOI Creative Commons
Julia Sehl‐Ewert, Virginia Friedrichs, Tessa Carrau

и другие.

Viruses, Год журнала: 2023, Номер 15(3), С. 729 - 729

Опубликована: Март 11, 2023

African swine fever (ASF) is a severe, globally important disease in domestic and wild pigs. The testing of alternative transmission routes has proven that the ASF virus (ASFV) can be efficiently transmitted to sows via semen from infected boars through artificial insemination. Boars intramuscularly inoculated with ASFV strain “Estonia 2014” showed grossly microscopically visible changes testis, epididymis, prostate, vesicular gland. gross lesions included hemorrhages on scrotum, testicular membranes, parenchyma; edema; hydroceles; proliferations tunica vaginalis. Histopathologically, vasculitis perivasculitis was detected testis epididymis. Subacutely animals further revealed degeneration epididymal tubules, pointing destruction blood–testis blood–epididymis barriers upon progression. This confirmed by evidence round cells sperm abnormalities at later time points after infection. histopathology associated presence viral DNA infectious virus, limited amount antigens. In most scenarios, impact these reproductive performance long-term persistence probably negligible due culling animals. However, under backyard conditions boar populations, males will remain population fate should evaluated.

Язык: Английский

Процитировано

2
Impact of ASFV Detergent Inactivation on Biomarkers in Serum and Saliva Samples DOI Creative Commons
Lorena Franco‐Martínez, Martin Beer, Silvia Martı́nez-Subiela

и другие.

Pathogens, Год журнала: 2022, Номер 11(7), С. 750 - 750

Опубликована: Июнь 30, 2022

African swine fever (ASF) is a notifiable viral disease of domestic and wild suids. Despite intensive research efforts, the pathogenesis still far from being understood. Analysis biomarkers in different body fluids may supplement traditional studies. As reliable protocols are often established laboratories with lower biosafety, inactivation samples crucial. The objective this study was to find procedure that inactivates virus while preserving for downstream analyses. To means, three were employed, namely Tergitol-type NP-40 (NP-40), polyoxyethylene-p-t-octylphenol (Triton X-100) one 95 °C heating. It could be demonstrated all treated 0.5% (v/v) concentration both detergents showed an absence infectivity. same true heated samples. However, serum not suitable Next, impact treatment on biomarker readouts assessed. While had detection biomarkers, correlation retained. In particular, desired detergent more accurate measurements achieving efficient inactivation. Based these studies, can reliably inactivated most analyses, thus broader interdisciplinary cooperation possible.

Язык: Английский

Процитировано

2
Inactivation Protocols for African Swine Fever Virus in Serum and Saliva Samples DOI Open Access
Lorena Franco‐Martínez, Martin Beer, Silvia Martı́nez-Subiela

и другие.

Опубликована: Май 30, 2022

African swine fever (ASF) is a notifiable viral disease of domestic and wild suids. Despite intensive re-search efforts, the pathogenesis still far from being understood. Analysis biomarkers in different body fluids may supplement traditional studies. As reliable protocols are often es-tablished laboratories with lower biosafety, inactivation samples crucial. The objective this study was to find procedure that inactivates virus while preserving for down-stream analyses. To means, three were employed, namely Tergitol-type NP-40 (NP-40) polyoxyethylene-p-t-octylphenol (Triton X-100), respectively, one 95 °C heating. It could be demonstrated all treated 0.5% (v/v) concentration both deter-gents showed absence infectivity. same true heated samples. However, serum not suitable Next, treatment impact on biomarker readouts assessed. While had an detection biomarkers, correlation retained. Especially desired detergent more accurate measurements achieving efficient inactivation. Based these studies, can reliably inactivated most analyses thus broader interdisciplinary cooperation possible.

Язык: Английский

Процитировано

0