Regulatory Mechanisms in Biosystems,
Год журнала:
2024,
Номер
15(4), С. 894 - 899
Опубликована: Окт. 24, 2024
The
main
purpose
of
the
investigation
is
to
determine
toxic
effects
alkaloid-rich
extract
seeds
Datura
innoxia
Mill.
on
liver
tissue
white
laboratory
rats.
an
annual
herb
belonging
Solanaceae
family
and
widely
distributed
in
territory
Republic
Azerbaijan.
species
are
known
as
a
source
tropane
alkaloids,
which
have
therapeutic
effects.
primary
symptoms
poisoning,
related
anticholinergic
include
hallucinations,
mydriasis,
dry
skin,
dizziness,
tachycardia,
reduced
urinary
retention,
etc.
various
pathological
changes
living
organisms
due
toxicity
alkaloids
were
detected
by
different
groups
researchers.
In
presented
study
plant
was
prepared
using
acid-base
extraction
method
dissolved
0.9%
physiological
saline.
Animals
subjected
oral
administration
for
period
30
days
at
dose
5
mg/kg
body
weight,
once
daily.
At
end
experimental
study,
samples
obtained
from
control
groups.
Araldite-Epon
blocks
following
established
protocols
electron
microscopy,
semi-thin
ultrathin
sections
Leica
EM
UC7
ultramicrotome.
stained
examined
under
Primo
Star
light
microscope
JEM-1400
transmission
(TEM).
results
revealed
increased
vascular
permeability
damage
endothelial
cells
central
veins
sinusoids.
Edema
formation
observed
periendothelial
perivascular
spaces.
Stagnation
sinusoidal
lumen
presence
bridge-like
connections
among
majority
sinusoids
identified.
Necrosis
spaces
veins.
membranes
hepatocytes,
constitute
parenchyma
liver,
damaged,
cytoplasmic
organelles
migrated
intercellular
Disse
Glycogen
cytoplasm
hepatocytes
transformed
into
amorphous
form,
with
certain
nuclei
experiencing
dystrophy,
tight
junction
bile
canaliculus
disrupted,
sometimes
not
visible.
identified
indicate
that
utilization
over
resulted
Ecotoxicology and Environmental Safety,
Год журнала:
2025,
Номер
289, С. 117694 - 117694
Опубликована: Янв. 1, 2025
Arsenic
in
the
environment,
such
as
sodium
arsenic
(NaAsO2),
is
a
frequently
occurring
hazard
that
has
been
linked
to
nonalcoholic
steatohepatitis
(NASH).
Our
prior
research
established
involvement
of
ferroptosis
arsenic-induced
NASH,
but
precise
underlying
mechanisms
remain
elusive.
Here,
we
found
exposure
NaAsO2
had
suppressive
effect
on
expression
CDGSH
iron-sulfur
domain-containing
protein
2
(CISD2)
at
and
gene
levels,
overexpression
CISD2
inhibited
NaAsO2-induced
NASH.
Additionally,
administration
hepatocytes
triggered
mitochondrial
dysfunction,
manifesting
release
cytochrome
c,
impairment
respiratory
chain,
reduction
ATP
synthesis.
However,
these
adverse
effects
were
alleviated
through
CISD2.
Intracellular
iron
redistribution
was
induced
by
ferroptosis.
This
inhibition
characterized
cytoplasmic
levels
an
increase
levels.
study
demonstrated
intracellular
reorganization
dysfunction
inhibition,
leading
may
provide
novel
means
treatment
fatty
liver
disease
environmental
factors.
Applied Microbiology and Biotechnology,
Год журнала:
2025,
Номер
109(1)
Опубликована: Янв. 7, 2025
Abstract
Iron
oxide
nanoparticles,
recognized
for
their
superparamagnetic
properties,
are
promising
future
healthcare
therapies.
However,
extensive
use
in
medicine
and
electronics
contributes
to
discharge
into
our
environments,
highlighting
the
need
further
research
on
cellular
damage
effects
aquatic
organisms.
While
detrimental
properties
of
other
compounds
have
been
stated
early-life
stages
fish,
cytotoxic
consequences
iron
nanoparticles
(SPIONs)
these
still
unexplored.
Therefore,
using
red
tilapia
(
Oreochromis
sp.)
as
a
model
organism,
this
study
is
first
talk
about
subtle
alterations
caused
by
biologically
induced
biomineralized
Fe
3
O
4
-SPIONs
Bacillus
sp.
stages.
Once
eggs
were
fertilized,
they
challenged
different
doses
SPIONs
(0,
5,
10,
15,
30
mg/l),
tenfold
increases
(50,
100,
150,
300
mg/l)
72
h.
The
hatching
rate,
malformation
body
length,
deformities
larvae
all
studied.
Our
showed
that
harmful
early
life
embryos
larvae.
They
slowed
delay,
decrease
survival
an
increase
heart
bleeding,
arrested
development,
membrane
changed
axis’s
physiological
structure.
Additionally,
results
indicated
numerous
larvae,
with
lordosis,
kyphosis,
scoliosis
once
subjected
50
150
mg/l
concentrations,
respectively.
This
could
assist
us
recognizing
risk
evaluating
disrupting
potential
nanoparticles.
key
objective
inquiry
describe
existing
features
produced
magnetite
(29.44
g/l)
including
morphological,
chemical,
magnetic
characteristics.
Illustrate
current
role
medicinal
applications
organisms
studying
vivo
motivate
development
enhanced
systems.
As
recommendation,
more
needed
completely
understand
how
various
exposure
endpoints
disturb
bodies
Key
points
•
Biogenic
SPIONs:
material
future.
Characterization
essential
assess
functional
SPIONs.
-SPIONs’
impact
ontogeny.
Graphical
abstract
Ecotoxicology and Environmental Safety,
Год журнала:
2025,
Номер
292, С. 118016 - 118016
Опубликована: Март 1, 2025
Phenylthiourea
(PTU)
is
a
well-known
inhibitor
of
melanin
synthesis
that
has
been
extensively
utilized
in
ecotoxicological
studies
involving
zebrafish.
Although
there
are
reports
suggesting
PTU
may
influence
the
toxicity
various
compounds,
underlying
mechanisms
its
action
remain
unclear.
Bavachalcone
(BavaC)
wide
range
applications
agriculture
and
medicine,
it
can
enter
groundwater
through
variety
pathways
pose
risk
to
aquatic
ecosystems.
We
found
enhanced
hepatotoxicity
BavaC
zebrafish,
but
mechanism
was
In
this
study,
interactive
effects
200
μM
2.5
on
zebrafish
larvae
were
evaluated
after
72
h
exposure.
significantly
increased
BavaC-induced
hepatotoxicity,
which
characterized
by
liver
hypoplasia,
hepatocyte
vacuolation,
lipid
accumulation.
Matrix-assisted
laser
desorption
ionization
mass
spectrometry
imaging
(MALDI-MSI)
revealed
contents
fatty
acids,
phosphatidylcholine
glutathione
increased.
The
results
RNA
sequencing
(RNA-seq)
Real-time
PCR
(RT-qPCR)
analyses
indicated
several
metabolic
pathways,
including
xenobiotic
biodegradation
metabolism,
amino
acid
metabolism
carbohydrate
differentially
regulated
PTU+BavaC
group
compared
group.
Our
findings
indicate
PTU-induced
disorders
exacerbate
highlight
need
reconsider
use
zebrafish-based
assessments
environmental
pollutants.
