bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 21, 2024
Abstract
Background
Exosomal
microRNAs
(exomiRs),
transported
via
exosomes,
play
a
pivotal
role
in
intercellular
communication.
In
cancer,
exomiRs
influence
tumor
progression
by
regulating
key
cellular
processes
such
as
proliferation,
angiogenesis,
and
metastasis.
Their
mediating
communication
between
cancer
cells
the
microenvironment
highlights
their
significance
potential
diagnostic
therapeutic
targets.
Methodology
this
study,
we
aimed
to
characterize
of
influencing
pre-metastatic
niche
(PMN).
Across
7
types,
including
4
cell
lines
three
tumors,
extracted
high
confidence
(Log
FC
>=
2
exosomes
relative
control)
targets
(experimentally
identified
targeted
at
least
exomiRs).
Subsequently,
enriched
pathways
selected
top
100
high-confidence
exomiR
based
on
frequency
appearance
pathways.
These
were
consistently
used
throughout
analysis.
Results
Cancer
line
derived
ExomiRs
have
significantly
higher
GC
content
genomic
background.
Pathway
among
included
general
cancer-associated
“wound
healing”
“regulation
epithelial
proliferation”,
well
cancer-specific
processes,
angiogenesis
kidney”
(KIRC),
“ossification”
lung
(LUAD),
“positive
regulation
cytokine
production”
pancreatic
(PAAD).
Similarly,
‘Pathways
cancer’
‘MicroRNAs
ranked
10
KEGG
all
types.
ExomiR
not
only
for
suppressor
genes
(TSG)
but
are
also
downregulated
formed
lungs
compared
normal
lung.
Motif
analysis
shows
similarity
motifs
from
across
Our
recapitulates
associated
with
M2
macrophage
differentiation
chemoresistance
miR-21
miR-222-3p,
signaling
PTEN/PI3/Akt,
NF-κB,
etc.
Cox
regression
indicated
that
overall
survival
patients
TCGA.
Lastly,
Support
Vector
Machine
(SVM)
model
using
target
gene
expression
classified
responders
non-responders
neoadjuvant
chemotherapy
an
AUROC
0.96
(in
LUAD),
than
other
previously
reported
signatures.
Conclusion
study
characterizes
shaping
PMN
diverse
cancers,
underscoring
potential.
Cancer Cell International,
Год журнала:
2025,
Номер
25(1)
Опубликована: Май 25, 2025
Osteosarcoma
(OS),
chondrosarcoma
(CHS),
and
Ewing
sarcoma
(EWS)
are
the
main
types
of
bone
cancer
(BC).
OS
is
most
common
BC
in
this
group.
It
children
older
people,
especially
their
long
bones.
Treatments
for
sarcomas
tumors
have
slowly
improved,
so
researchers
began
looking
into
additional
alternative
approaches
to
standard
therapies.
Therefore,
ability
precisely
manipulate
metallic
nanoparticles
(MNPs)'
form,
size,
charge,
surface
modification
makes
them
very
useful
treating
cancer.
However,
due
biocompatibility
possible
toxicity
MNPs,
MNP
has
limits
clinical
use
BC.
green
synthesis
MNPs
achieved
by
bio-reducing
ions,
which
results
creation
NPs,
using
living
entities
or
extracts.
Green
derived
from
natural
sources
provide
a
secure
environmentally
responsible
solution.
Benefits
include
tailored
medicine
delivery
biocompatibility.
reduce
damage
healthy
cells
while
improving
targeting
cells.
In
study,
we
reviewed
how
different
synthesized
methods
can
help
treat
various
This
work
usual
way
making
BC,
problems
with
benefits
future
uses
synthetic
IGI Global eBooks,
Год журнала:
2025,
Номер
unknown, С. 283 - 324
Опубликована: Июнь 5, 2025
Adaptation
mediated
by
drug
resistance
is
one
of
the
barriers
to
combating
cancer.
Moreover,
during
stress
responses,
such
as
hypoxia
and
nutrient
deprivation,
mitophagy,
which
selective
autophagy
mitochondria,
plays
an
essential
role
in
influencing
cancer
cell
drugs.
This
chapter
describes
molecular
pathways
involved
mitophagy
their
effects
on
survival
cells
mechanisms.
The
latest
discoveries
listed
this
illustrate
how
facilitates
ability
adjust
metabolic
therapeutic
emergencies.
Modulation
established
a
promising
strategy
for
mediating
resistance.
future
studies
should
focus
development
targeted
therapies
alter
regulation
improve
treatment
outcomes.
Computational and Structural Biotechnology Journal,
Год журнала:
2024,
Номер
27, С. 252 - 264
Опубликована: Дек. 26, 2024
Exosomal
microRNAs
(exomiRs)
play
a
critical
role
in
intercellular
communication,
especially
cancer,
where
they
regulate
key
cellular
processes
like
proliferation,
angiogenesis,
and
metastasis,
highlighting
their
significance
as
potential
diagnostic
therapeutic
targets.
Here,
we
aimed
to
characterize
the
of
exomiRs,
derived
from
seven
cancer
types
(four
cell
lines
three
tumors),
influencing
pre-metastatic
niche
(PMN).
In
each
type
extracted
high
confidence
exomiRs
(LogFC
>=
2
exosomes
relative
control),
experimentally
validated
targets,
enriched
pathways
among
those
We
then
selected
top100
high-confidence
targets
based
on
frequency
appearance
pathways.
observed
significantly
higher
GC
content
genomic
background.
Gene
Ontology
analysis
revealed
both
general
processes,
such
wound
healing
epithelial
well
cancer-specific
"angiogenesis"
kidney
"ossification"
lung.
ExomiR
were
for
tumor
suppressor
genes
downregulated
PMN
formed
lungs
compared
normal.
Motif
showed
inter-cancer
similarity
motifs
exomiRs.
Our
recapitulated
associated
with
M2
macrophage
differentiation
chemoresistance,
miR-21
miR-222-3p,
regulating
signaling
PTEN/PI3/Akt,
NF-kB,
etc.
Additionally,
Cox
regression
TCGA
indicated
that
exomiR
are
better
overall
survival
patients.
Lastly,
support
vector
machine
model
using
gene
expression
classified
responders
non-responders
therapy
an
AUROC
ranging
0.72
0.96,
than
previously
reported
signatures.
Objectives:
Adrenocortical
carcinoma
(ACC),
a
rare
and
aggressive
adrenal
cortex
cancer,
poses
significant
challenges
due
to
high
mortality,
poor
prognosis,
early
post-surgery
recurrence.
Variability
in
survival
across
ACC
stages
emphasizes
the
need
uncover
its
molecular
underpinnings.
adenoma,
benign
tumor,
adds
diagnostic
challenges,
highlighting
necessity
for
insights.
The
Non-SMC
Associated
Condensin
Complex
(NCAP)
gene
family,
recognized
roles
chromosomal
structure
cell
cycle
control.
This
study
focuses
on
evaluating
NCAP
functions
importance
through
expression
profiling
identify
therapeutic
targets.
Methods:
Microarray
datasets
from
patients,
obtained
Gene
Expression
Omnibus
database,
were
normalized
eliminate
batch
effects.
Differential
analysis
of
family
genes,
facilitated
by
GEPIA2
included
pathological
stage
evaluations.
A
Protein-Protein
Interaction
network
was
constructed
using
GeneMANIA,
additional
insights
gained
Ontology
enrichment
analysis,
correlation
ROC
curve
analysis.
Results:
samples
exhibited
elevated
levels
NCAPG,
NCAPG2,
NCAPH
compared
normal
adenoma
samples.
Increased
these
genes
correlated
with
overall
survival,
particularly
advanced
disease
stages.
highlighted
interactions
related
proteins,
demonstrated
their
involvement
Differentially
expressed
showed
positive
associations,
indicated
discriminatory
power
identifying
Conclusion:
potential
ACC,
suggesting
tumor
aggressiveness
relevance.
These
could
serve
as
targets
markers
but
further
exploration
into
activities
validation
studies
is
imperative
fully
harness
potential,
advancing
precision
medicine
approaches
against
this
lethal
malignancy.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12524 - 12524
Опубликована: Ноя. 21, 2024
Glioblastoma
Multiforme
(GBM)
is
the
most
aggressive
primary
tumor
of
Central
Nervous
System
(CNS)
with
a
low
survival
rate.
The
malignancy
GBM
sustained
by
bidirectional
crosstalk
between
cells
and
Tumor
Microenvironment
(TME).
This
mechanism
intercellular
communication
mediated,
at
least
in
part,
release
exosomes.
Glioma-Derived
Exosomes
(GDEs)
work,
indeed,
as
potent
signaling
particles
promoting
progression
brain
tumors
inducing
proliferation,
invasion,
migration,
angiogenesis
resistance
to
chemotherapy
or
radiation.
Given
their
nanoscale
size,
exosomes
can
cross
blood-brain
barrier
(BBB),
thus
becoming
not
only
promising
biomarker
predict
diagnosis
prognosis
but
also
therapeutic
target
treat
GBM.
In
this
review,
we
describe
structural
functional
characteristics
involvement
development,
diagnosis,
treatment.
addition,
discuss
how
be
modified
used
target/drug
delivery
system
for
clinical
applications.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 21, 2024
Abstract
Background
Exosomal
microRNAs
(exomiRs),
transported
via
exosomes,
play
a
pivotal
role
in
intercellular
communication.
In
cancer,
exomiRs
influence
tumor
progression
by
regulating
key
cellular
processes
such
as
proliferation,
angiogenesis,
and
metastasis.
Their
mediating
communication
between
cancer
cells
the
microenvironment
highlights
their
significance
potential
diagnostic
therapeutic
targets.
Methodology
this
study,
we
aimed
to
characterize
of
influencing
pre-metastatic
niche
(PMN).
Across
7
types,
including
4
cell
lines
three
tumors,
extracted
high
confidence
(Log
FC
>=
2
exosomes
relative
control)
targets
(experimentally
identified
targeted
at
least
exomiRs).
Subsequently,
enriched
pathways
selected
top
100
high-confidence
exomiR
based
on
frequency
appearance
pathways.
These
were
consistently
used
throughout
analysis.
Results
Cancer
line
derived
ExomiRs
have
significantly
higher
GC
content
genomic
background.
Pathway
among
included
general
cancer-associated
“wound
healing”
“regulation
epithelial
proliferation”,
well
cancer-specific
processes,
angiogenesis
kidney”
(KIRC),
“ossification”
lung
(LUAD),
“positive
regulation
cytokine
production”
pancreatic
(PAAD).
Similarly,
‘Pathways
cancer’
‘MicroRNAs
ranked
10
KEGG
all
types.
ExomiR
not
only
for
suppressor
genes
(TSG)
but
are
also
downregulated
formed
lungs
compared
normal
lung.
Motif
analysis
shows
similarity
motifs
from
across
Our
recapitulates
associated
with
M2
macrophage
differentiation
chemoresistance
miR-21
miR-222-3p,
signaling
PTEN/PI3/Akt,
NF-κB,
etc.
Cox
regression
indicated
that
overall
survival
patients
TCGA.
Lastly,
Support
Vector
Machine
(SVM)
model
using
target
gene
expression
classified
responders
non-responders
neoadjuvant
chemotherapy
an
AUROC
0.96
(in
LUAD),
than
other
previously
reported
signatures.
Conclusion
study
characterizes
shaping
PMN
diverse
cancers,
underscoring
potential.
