Metabolic Engineering for Squalene Production: Advances and Perspectives
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 3, 2024
Squalene
is
a
linear
polyunsaturated
triterpene
which
has
multiple
physiological
functions
including
anticancer,
antioxidant,
and
skin-care.
It
been
widely
used
in
the
food,
medicine,
cosmetics
sectors
also
serves
as
precursor
of
triterpenes
steroids.
Recently,
production
squalene
by
microbial
cell
hosts
drawn
much
attention
due
to
its
sustainability,
environmental
friendliness,
great
efficiency.
In
this
review,
we
first
introduce
recent
developments
employing
factories,
especially
yeasts.
Next,
underscore
primary
metabolic
strategies,
biosynthetic
pathway
engineering,
manipulation,
cofactor
organelle
engineering.
addition
traditional
engineering
discuss
some
prospective
regulation
approaches,
lipid
synthesis,
identifying
manipulating
related
transcription
factors,
dynamic
pathway,
secretion
membrane-impermeable
terpenoids.
These
approaches
provide
insights
for
further
Язык: Английский
Potential of oleaginous microbes for lipid accumulation and renewable energy generation
World Journal of Microbiology and Biotechnology,
Год журнала:
2024,
Номер
40(11)
Опубликована: Окт. 3, 2024
Язык: Английский
Novel cell factory for the production of 24-epi-ergosterol, an un-natural semi-synthetic precursor for the production of brassinolide in Yarrowia lipolytica
World Journal of Microbiology and Biotechnology,
Год журнала:
2025,
Номер
41(3)
Опубликована: Март 1, 2025
Язык: Английский
Multidimensional insights into squalene epoxidase drug development: in vitro mechanisms, in silico modeling, and in vivo implications
Expert Opinion on Therapeutic Targets,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 30, 2025
Squalene
epoxidase
(SQLE)
is
a
pivotal
enzyme
in
sterol
biosynthesis,
catalyzing
the
conversion
of
squalene
to
2,3-oxidosqualene.
Beyond
its
core
role
cholesterol
homeostasis,
SQLE
implicated
cancer,
hypercholesterolemia,
and
fungal
infections,
positioning
it
as
valuable
therapeutic
target.
We
conducted
comprehensive
literature
search
across
primary
databases
gather
vitro,
silico,
vivo
evidence
on
SQLE.
This
review
explores
enzyme's
structural
functional
features,
including
substrate
specificity
catalytic
mechanisms,
examines
inhibitor
interactions.
Computational
methods
predict
-
dynamics,
guiding
drug
design,
while
investigations
clarify
SQLE's
metabolic
disorders
tumorigenesis.
Challenges
include
resistance
study
discrepancies,
but
emerging
technologies,
such
cryo-electron
microscopy
CRISPR
editing,
offer
new
avenues
for
deeper
exploration.
an
underexplored
yet
promising
target,
with
particular
relevance
oxidative
stress,
ferroptosis,
gut
microbiota
research.
Overcoming
current
barriers
through
advanced
technologies
multidisciplinary
strategies
could
propel
SQLE-targeted
treatments
into
clinical
practice,
supporting
precision
medicine
broader
translational
applications.
Язык: Английский