Genes,
Год журнала:
2023,
Номер
14(12), С. 2229 - 2229
Опубликована: Дек. 17, 2023
Since
the
introduction
of
new
molecular
techniques,
diagnostic
landscape
soft
tissue
and
bone
tumors
has
expanded
greatly
over
past
few
years.
The
use
techniques
led
to
identification
genetic
alterations
and,
therefore,
a
better
understanding
tumorigenesis,
tumor
detection
classification.
Furthermore,
methylation
profiling
emerged
as
classification
tool
for
tumors.
Molecular
pathology
also
plays
an
important
role
in
determination
patient
prognosis
targets
that
can
be
used
targeted
therapy.
As
result,
gained
more
prominent
daily
practice
surgical
pathologist.
This
review
delves
into
various
applied
It
highlights
their
applications
through
analysis
five
specific
cases.
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 282 - 282
Опубликована: Янв. 27, 2025
This
paper
explores
various
aspects
of
microbiology
and
immunology,
with
a
particular
focus
on
the
epidemiology
molecular
characterisation
infectious
diseases
in
Caribbean
South
America.
Key
areas
investigation
include
tuberculosis
(TB),
experimental
vaccines,
bloodborne
pathogens.
A
retrospective
study
conducted
Jamaica
highlights
significance
early
HIV
screening,
timely
diagnosis,
inte-grated
care.
The
also
examines
challenges
posed
by
nosocomial
infections,
particularly
those
caused
antibiotic-resistant
Gram-negative
bacteria
methicillin-resistant
Staphylococcus
aureus
(MRSA),
emphasising
critical
importance
infection
control
measures.
Additionally,
it
regional
microbiome,
global
response
to
diseases,
immune
responses
patients
immunodeficiency
disorders
such
as
severe
combined
(SCID)
chronic
granulomatous
disease
(CGD),
underscoring
their
heightened
susceptibility
wide
range
infections.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 26, 2025
DNA
serves
as
the
foundation
for
molecular
biology,
leading
to
development
of
numerous
techniques.
Often,
these
techniques
necessitate
separation
and
visualization
specific
regions.
Electrophoresis
provides
a
solution
this
requirement.
However,
purification
from
agarose
gels
presents
significant
challenge,
both
in
terms
complexity
cost.
Therefore,
here
we
propose
two
main
protocols
that
are
cost-effective
efficient
based
on
silica
columns
or
freezing
follow
by
alcohol
precipitation.
In
case
column
extraction,
gel
was
partially
completely
dissolved,
yielding
most
situations.
extraction
precipitation
with
ethanol,
obtained
only
out
three
treatments.
A
successful
bacterial
transformation
PCR
were
achieved
confirmed
suitability
recovered
further
applications.
Diagnostics,
Год журнала:
2025,
Номер
15(8), С. 1044 - 1044
Опубликована: Апрель 20, 2025
Multiplex
Polymerase
Chain
Reaction
(PCR)
has
significantly
impacted
the
field
of
infectious
disease
diagnostics,
offering
rapid
and
precise
identification
bacterial
fungal
pathogens.
Unlike
traditional
culture
methods,
which
may
take
days
to
yield
results,
multiplex
PCR
provides
diagnostic
insights
within
hours,
enabling
faster,
targeted
antimicrobial
therapy
reducing
delay
in
treating
critical
infections
like
sepsis.
The
technique’s
high
sensitivity
broad
pathogen
coverage
make
it
ideal
for
both
single
polymicrobial
infections,
improving
outcomes
across
respiratory,
bloodstream,
bacterial/fungal
infections.
However,
is
not
without
challenges;
initial
costs
need
specialized
training
can
limit
its
adoption,
especially
low-resource
settings.
This
review
discusses
clinical
advantages
limitations
PCR,
highlighting
influence
on
accuracy,
stewardship,
global
fight
against
resistance
(AMR).
Furthermore,
recent
innovations
such
as
digital
portable
devices,
are
explored
potential
tools
expanding
access
diagnostics
worldwide.
Viruses,
Год журнала:
2024,
Номер
16(5), С. 765 - 765
Опубликована: Май 11, 2024
(1)
Goose
astrovirus
(GAstV)
is
a
novel
emerging
pathogen
that
causes
significant
economic
losses
in
waterfowl
farming.
A
convenient,
sensitive,
and
specific
detection
method
for
GAstV
field
samples
important
order
to
effectively
control
GAstV.
Droplet
digital
polymerase
chain
reaction
(ddPCR)
novel,
good-precision,
absolute
quantitation
PCR
technology
which
does
not
require
calibration
curves.
(2)
In
this
study,
we
developed
ddPCR
system
the
sensitive
accurate
quantification
of
using
conserved
region
ORF2
gene.
(3)
The
limit
was
10
copies/µL,
~28
times
greater
sensitivity
than
quantitative
real-time
(qPCR).
specificity
test
determined
by
failure
amplification
other
avian
viruses.
Both
qPCR
tests
showed
good
repeatability
linearity,
established
had
high
Clinical
sample
results
positive
rate
(88.89%)
higher
(58.33%).
(4)
As
result,
our
suggest
newly
might
offer
improved
analytical
its
measurements.
could
be
widely
applied
clinical
infections.
Aging Medicine,
Год журнала:
2024,
Номер
7(2), С. 202 - 213
Опубликована: Фев. 20, 2024
Abstract
The
precise
identification
of
Alzheimer's
disease
and
other
prevalent
neurodegenerative
diseases
remains
a
difficult
issue
that
requires
the
development
early
detection
inexpensive
biomarkers
can
replace
present
cerebrospinal
fluid
imaging
biomarkers.
Blood
biomarkers,
such
as
amyloid
neurofilament
light,
have
been
emphasized
an
important
practical
tool
in
testing
or
examination
procedure
thanks
to
advancements
ultra‐sensitive
techniques.
Although
saliva
is
not
currently
being
researched
for
diseases,
it
source
be
used
has
some
advantages
over
biofluids.
While
this
may
true
most
people,
getting
from
elderly
people
presents
significant
challenges.
In
overview,
we
will
first
discuss
how
created
aging‐related
illnesses
affect
amount
kind
produced.
findings
support
use
salivary
protein,
tau
species,
novel
diagnosis
disease.
Graphene-based
biosensors
(GBBs)
have
distinct
electrochemical,
mechanical,
and
thermal
properties,
making
them
attractive
candidates
for
a
wide
range
of
biological
applications.
Since
the
discovery
graphene,
GBBs
been
used
in
research
led
way
development
innovative
diagnostic
methods
diseases,
including
dementia.
Techniques
GBB
synthesis
functionalization
are
covered
number
reviews.
In
contrast,
focus
this
review
is
on
most
current
scientific
developments
dementia,
particularly
with
regard
to
diagnosis
treatment.
The
major
characteristics
dementia
briefly
described,
then
creation
high-performance
dementia-sensing
electrodes,
their
analytical
results,
interference
tests,
ranking
according
limit
detection
samples
all
highlighted.
Moreover,
proved
best
detection.
From
clinical
perspective,
graphene-based
biosensing
tools
appropriate
point-of-care
testing
tailored
management.
industry,
being
developed
into
smartphone-connected
intelligent
disease
management
solutions.
BMC Pregnancy and Childbirth,
Год журнала:
2024,
Номер
24(1)
Опубликована: Июль 11, 2024
Abstract
Background
Digital
Polymerase
Chain
Reaction
(dPCR)
presents
a
promising
approach
for
quantifying
DNA
and
analyzing
copy
number
variants,
particularly
in
non-invasive
prenatal
testing.
This
method
offers
streamlined
time-efficient
procedure
contrast
to
the
widely
used
next-generation
sequencing
Studies
have
reported
encouraging
results
dPCR
detecting
fetal
autosomal
aneuploidies.
Consequently,
this
systematic
review
aimed
evaluate
effectiveness
of
screening
trisomy
21,
18,
13.
Methods
A
search
was
conducted
PubMed,
Web
Sciences,
Embase
relevant
articles
published
up
December
30,
2023.
The
Quality
Assessment
Diagnostic
Accuracy
Studies-2
(QUADAS-2)
utilized
quality
assessment
included
articles.
Furthermore,
bivariate
random-effect
regression
model
conduct
meta-analysis
on
utility
21
screening.
Results
total
9
were
review,
with
all
them
assessing
screening,
2
1
studies
conducting
additional
analysis
abilities
18
13,
respectively.
random-effects
calculated
pooled
sensitivity
specificity
95%
confidence
interval
(CI).
Meta-analysis
6
comparing
trisomy-21
karyotyping
demonstrated
dPCR's
98%
[95%
CI:
94
-100]
99%
99
-100].
While
13
proved
impractical,
values
favorable.
Conclusions
These
findings
suggest
that
holds
promise
as
an
effective
tool
testing,
presenting
less
time-consuming
intricate
alternative
sequencing.
However,
further
research
is
necessary
applicability
clinical
settings
delineate
its
specific
advantages
over
study
contributes
valuable
insights
into
potential
enhancing
methodologies.
Trial
registration
protocol
registered
International
Prospective
Register
Systematic
Reviews
(PROSPERO)
7/3/2024,
code
CRD42024517523.
