Biomaterials, Год журнала: 2024, Номер 309, С. 122585 - 122585
Опубликована: Апрель 27, 2024
Язык: Английский
Bone Research, Год журнала: 2023, Номер 11(1)
Опубликована: Дек. 20, 2023
The skeleton is a highly innervated organ in which nerve fibers interact with various skeletal cells. Peripheral endings release neurogenic factors and sense signals, mediate bone metabolism pain. In recent years, tissue engineering has increasingly focused on the effects of nervous system regeneration. Simultaneous regeneration nerves through use materials or by enhancement endogenous repair signals been proven to promote functional Additionally, emerging information mechanisms interoception central regulation homeostasis provide an opportunity for advancing biomaterials. However, comprehensive reviews this topic are lacking. Therefore, review provides overview relationship between regeneration, focusing applications. We discuss novel regulatory explore innovative approaches based nerve-bone interactions Finally, challenges future prospects field briefly discussed.
Язык: Английский
Процитировано
45
Bone Research, Год журнала: 2024, Номер 12(1)
Опубликована: Янв. 23, 2024
Abstract Osteoporosis is a widely observed condition characterized by the systemic deterioration of bone mass and microarchitecture, which increases patient susceptibility to fragile fractures. The intricate mechanisms governing homeostasis are substantially impacted extracellular vesicles (EVs), play crucial roles in both pathological physiological contexts. EVs derived from various sources exert distinct effects on osteoporosis. Specifically, released osteoblasts, endothelial cells, myocytes, mesenchymal stem cells contribute formation due their unique cargo proteins, miRNAs, cytokines. Conversely, secreted osteoclasts immune promote resorption inhibit formation. Furthermore, use as therapeutic modalities or biomaterials for diagnosing managing osteoporosis promising. Here, we review current understanding impact homeostasis, including classification biogenesis regulatory present an overview latest research progress treating using EVs. Finally, discuss challenges prospects translational
Язык: Английский
Процитировано
32
Theranostics, Год журнала: 2024, Номер 14(5), С. 2099 - 2126
Опубликована: Янв. 1, 2024
Exosomes, which are small vesicles enclosed by a lipid bilayer and released many cell types, widely dispersed have garnered increased attention in the field of regenerative medicine due to their ability serve as indicators diseases agents with therapeutic potential. Exosomes play crucial role mediating intercellular communication through transfer biomolecules, including proteins, lipids, RNA, other molecular constituents, between cells. The targeted transport proteins nucleic acids specific cells has potential enhance or impair biological functions. applications, they can be used alone combination approaches. examination unique attributes functions these factors emerged prominent study realm biomedical research. This manuscript summarizes origins properties exosomes, structural, biological, physical, chemical aspects. paper offers complete recent progress tissue repair medicine, emphasizing possible implications methods forthcoming regeneration attempts.
Язык: Английский
Процитировано
23
Nano TransMed, Год журнала: 2024, Номер 3, С. 100033 - 100033
Опубликована: Фев. 4, 2024
Maxillofacial bone defects, arising from congenital malformations, tumors, infections, and traumas, represent a common clinical condition that impairs patients' appearance, speech, motor functions. Calcium phosphate nanoceramics, which bear chemical composition similar to autologous bone, demonstrate strong biocompatibility osteoconductivity, making them an extensively applied material in defect repairing. Notably, these nanoceramics exhibit inherent osteoinductive properties ,which can stimulate osteogenesis at non-osseous sites ,without the need for additional cells or growth factors, aspects further expand their application repair. Nevertheless, precise mechanism of calcium remains elusive, differences vivo individual persist, thereby academic communities resolve pressing issues restrict use. This paper reviews advancements research on factors affecting biological process formation, aiming provide new insights treatment maxillofacial defects.
Язык: Английский
Процитировано
21
Military Medical Research, Год журнала: 2025, Номер 12(1)
Опубликована: Март 3, 2025
Abstract Bone tissue relies on the intricate interplay between blood vessels and nerve fibers, both are essential for many physiological pathological processes of skeletal system. Blood provide necessary oxygen nutrients to bone tissues, remove metabolic waste. Concomitantly, fibers precede during growth, promote vascularization, influence cells by secreting neurotransmitters stimulate osteogenesis. Despite critical roles components, current biomaterials generally focus enhancing intraosseous vessel repair, while often neglecting contribution nerves. Understanding distribution main functions in is crucial developing effective engineering. This review first explores anatomy highlighting their vital embryonic development, metabolism, repair. It covers innovative regeneration strategies directed at accelerating intrabony neurovascular system over past 10 years. The issues covered included material properties (stiffness, surface topography, pore structures, conductivity, piezoelectricity) acellular biological factors [neurotrophins, peptides, ribonucleic acids (RNAs), inorganic ions, exosomes]. Major challenges encountered neurovascularized materials clinical translation have also been highlighted. Furthermore, discusses future research directions potential developments aimed producing repair that more accurately mimic natural healing tissue. will serve as a valuable reference researchers clinicians novel into practice. By bridging gap experimental practical application, these advancements transform treatment defects significantly improve quality life patients with bone-related conditions.
Язык: Английский
Процитировано
3
Frontiers in Pharmacology, Год журнала: 2025, Номер 16
Опубликована: Янв. 29, 2025
Schwann cells are specific peripheral glial with remarkable plasticity following nerve injury. Injury responses stimulate c-Jun activation in cells, drive epithelial-mesenchymal transition and cellular phenotypic changes, induce the generation of reprogrammed repair to orchestrate regeneration process. and/or cell-derived molecules commonly used as supporting neurotrophic factors construct cell-based tissue-engineered grafts for repairing severe injury long defects. Transplantation also serves a helpful approach treatment other injured tissues, such spinal cord, skin, digit tip, bone. not only associated tissue but involved tumorigenesis tumor progression. major component neurofibromatosis type 1 sole cell 2 schwannomatosis. In addition, function an important player microenvironment aid growth invasiveness many solid cancers. present review, we outline physiological pathological activities discuss functional roles homeostasis, regeneration, cancer.
Язык: Английский
Процитировано
2
ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(17), С. 20661 - 20676
Опубликована: Апрель 21, 2023
Organic-inorganic composites with high specific surface area and osteoinductivity provide a suitable microenvironment for cell ingrowth effective ossification, which could greatly promote bone regeneration. Here, we report gelatin methacryloyl (GelMA) cryogel microspheres that are reinforced hydroxyapatite (HA) nanowires calcium silicate (CS) nanofibers to achieve the goal. The prepared composite open porous structure rough facilitate anchoring, simultaneously exhibiting excellent injectability. Compared only HA- or CS-containing counterparts, GelMA composited HA:CS (termed as GMHC) sustained release of bioactive Ca, P, Si elements, conducive osteogenic differentiation marrow mesenchymal stromal cells (BMSCs). These can prevent from forming peralkalic conditions, is beneficial growth. After injection into rat calvarial defects, neo-bone tissue grows their pores, showing tight integration. embedded bioceramic components significantly regeneration, GMHC achieving best regenerative outcomes. Promisingly, organic-inorganic microspheres, area, biodegradability, osteoinductivity, act injectable microscaffolds repair defects enhanced efficiency, may widen scaffold strategy engineering.
Язык: Английский
Процитировано
43
Bioactive Materials, Год журнала: 2023, Номер 27, С. 377 - 393
Опубликована: Апрель 21, 2023
Crosstalk between nerves and bone is essential for repair, which Schwann cells (SCs) are crucial in the regulation of microenvironment. Considering that exosomes critical paracrine mediators intercellular communication exert important effects tissue aim this study to confirm function molecular mechanisms cell-derived (SC-exos) on regeneration propose engineered constructs simulate SC-mediated nerve–bone crosstalk. SCs promoted proliferation differentiation marrow mesenchymal stem (BMSCs) through exosomes. Subsequent mechanism studies demonstrated SC-exos BMSC osteogenesis by regulating TGF-β signaling pathway via let-7c-5p. Interestingly, migration tube formation performance endothelial progenitor cells. Furthermore, [email protected]/S were developed bioprinting technology simulated crosstalk improved microenvironment releasing SC-exos, exerting regulatory effect promote innervation, vascularization, thus effectively improving repair a cranial defect model. This demonstrates role underlying provides new strategy repair.
Язык: Английский
Процитировано
41
PubMed, Год журнала: 2023, Номер 4(4), С. 248 - 269
Опубликована: Янв. 1, 2023
While bone tissue is known for its inherent regenerative abilities, various pathological conditions and trauma can disrupt meticulously regulated processes of formation resorption. Bone engineering aims to replicate the extracellular matrix as well sophisticated biochemical mechanisms crucial effective regeneration. Traditionally, field has relied on external agents like growth factors pharmaceuticals modulate these processes. Although efficacious in certain scenarios, this strategy compromised by limitations such safety issues transient nature compound release half-life. Conversely, bioactive elements zinc (Zn), magnesium (Mg) silicon (Si), have garnered increasing interest their therapeutic benefits, superior stability, reduced biotic risks. Moreover, are often incorporated into biomaterials that function multifaceted components, facilitating regeneration via on-demand. By elucidating mechanistic roles efficacy elements, review establish a robust clinically viable advanced
Язык: Английский
Процитировано
27
Glia, Год журнала: 2024, Номер 72(4), С. 692 - 707
Опубликована: Янв. 8, 2024
Abstract Schwann cells (SCs), the primary glial of peripheral nervous system, which have been identified in many solid tumors, play an important role cancer development and progression by shaping tumor immunoenvironment supporting metastases. Using different cellular, molecular, genetic approaches with integrated bioinformatics analysis functional assays, we revealed human SC‐derived exosomal miRNAs lung vitro vivo. We found that miRNA‐21 from SCs up‐regulated proliferation, motility, invasiveness vitro, requires Rab small GTPases Rab27A Rab27B for exosome release. also SC miRNA‐21‐5p regulated activation targeting metalloprotease inhibitor RECK cells. Integrated bioinformatic analyses showed hsa‐miRNA‐21‐5p is associated poor prognosis patients adenocarcinoma can promote through multiple signaling pathways including MAPK, PI3K/Akt, TNF signaling. Furthermore, mouse xenograft models, exosomes augmented cell growth lymph node metastasis vivo . Together our data revealed, first time, SC‐secreted promoted spreading Thus, may oncogenic SC‐accelerated this pathway serve as a new therapeutic target further evaluation.
Язык: Английский
Процитировано
18