Aging and Disease,
Год журнала:
2024,
Номер
unknown, С. 0 - 0
Опубликована: Янв. 1, 2024
Fatigue,
commonly
experienced
in
daily
life,
is
a
feeling
of
extreme
tiredness,
shortage
or
lack
energy,
exhaustion,
and
difficulty
performing
voluntary
tasks.
Central
fatigue,
defined
as
progressive
failure
to
voluntarily
activate
the
muscle,
typically
linked
moderate-
light-intensity
exercise.
However,
some
instances,
high-intensity
exercise
can
also
trigger
onset
central
fatigue.
Exercise-induced
fatigue
often
precedes
decline
physical
performance
well-trained
athletes.
This
leads
reduction
nerve
impulses,
decreased
neuronal
excitability,
an
imbalance
brain
homeostasis,
all
which
adversely
impact
athlete's
longevity
their
sports
career.
Therefore,
implementing
strategies
delay
exercise-induced
vital
for
enhancing
athletic
safeguarding
athletes
from
debilitating
effects
In
this
review,
we
discuss
structural
basis,
measurement
methods,
biomarkers
Furthermore,
propose
non-pharmacological
interventions
mitigate
its
effects,
potentially
foster
improvements
athletes'
performances
healthful
sustainable
manner.
Subcellular
mitochondria
serve
as
sensors
for
energy
metabolism
and
redox
balance,
the
dynamic
regulation
of
functional
dysfunctional
plays
a
crucial
role
in
determining
cells'
fate.
Selective
removal
at
subcellular
level
can
provide
chondrocytes
with
to
prevent
degeneration,
thereby
treating
osteoarthritis.
Herein,
achieve
an
ideal
therapy,
cartilage
affinity
peptide
(WYRGRL)-decorated
liposomes
loaded
mitophagy
activator
(urolithin
A)
were
integrated
into
hyaluronic
acid
methacrylate
hydrogel
microspheres
through
microfluidic
technology,
named
HM@WY-Lip/UA,
that
could
efficiently
target
selectively
remove
mitochondria.
As
result,
this
system
demonstrated
advantage
function
restoration,
reactive
oxygen
species
scavenging,
cell
survival
rescue,
chondrocyte
homeostasis
maintenance
increasing
mitophagy.
In
rat
post-traumatic
osteoarthritis
model,
intra-articular
injection
HM@WY-Lip/UA
ameliorated
matrix
degradation,
osteophyte
formation,
subchondral
bone
sclerosis
8
weeks.
Overall,
study
indicated
provided
protective
effect
on
degeneration
efficacious
clinically
relevant
manner,
mitochondrial-oriented
strategy
has
great
potential
therapy
Cell Biology and Toxicology,
Год журнала:
2025,
Номер
41(1)
Опубликована: Фев. 24, 2025
Human
umbilical
cord
mesenchymal
stem
cell-derived
small
extracellular
vesicles
(hucMSC-sEV)
have
recently
garnered
attention
as
a
potential
therapeutic
approach
for
kidney
diseases
with
anti-inflammatory
effects.
Infiltrated
macrophages
play
an
important
role
in
facilitating
tissue
regeneration.
However,
the
intricate
regulatory
effects
of
hucMSC-sEV
on
during
cisplatin-induced
acute
injury
(AKI)
remain
unknown.
In
this
study,
we
uncovered
that
exhibited
potent
anti-inflammation
and
effectively
inhibited
polarization
M1
phenotype
macrophages.
Mechanically,
miRNA
sequencing
analysis
qRT-PCR
indicated
novel
miRNA,
named
miR-13896,
was
enriched
hucMSC-sEV.
When
transfected
miR-13896
mimic,
displayed
M2
elevated
levels
Arg1
IL-10,
while
inhibitor
promoted
phenotype.
Furthermore,
firstly
established
repressed
Tradd
expression
by
targeting
its
3'
untranslated
region
subsequently
NF-κB
signaling
pathway
Additionally,
to
improve
effects,
were
engineered
through
electroporation,
which
resulted
promoting
macrophages,
inhibiting
inflammatory
factors,
enhancing
repair.
Conclusively,
our
findings
provide
insights
into
mechanisms
underlying
AKI,
also
highlighting
electroporation
promising
strategy
treating
AKI.
ACS Nano,
Год журнала:
2024,
Номер
18(16), С. 10667 - 10687
Опубликована: Апрель 9, 2024
Cartilage
injuries
are
escalating
worldwide,
particularly
in
aging
society.
Given
its
limited
self-healing
ability,
the
repair
and
regeneration
of
damaged
articular
cartilage
remain
formidable
challenges.
To
address
this
issue,
nanomaterials
leveraged
to
achieve
desirable
outcomes
by
enhancing
mechanical
properties,
optimizing
drug
loading
bioavailability,
enabling
site-specific
targeted
delivery,
orchestrating
cell
activities
at
nanoscale.
This
review
presents
a
comprehensive
survey
recent
research
nanomedicine
for
repair,
with
primary
focus
on
biomaterial
design
considerations
advances.
The
commences
an
introductory
overview
intricate
microenvironment
further
delves
into
key
parameters
crucial
treating
damage,
including
microstructure,
surface
charge,
active
targeting.
focal
point
lies
advances
nano
delivery
systems
nanotechnology-enabled
3D
matrices
repair.
We
discuss
compositions
properties
these
elucidate
how
materials
impact
cartilage.
underscores
pivotal
role
nanotechnology
improving
efficacy
biomaterials
utilized
treatment
damage.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(30)
Опубликована: Май 1, 2024
Abstract
Osteoarthritis
(OA),
a
prevalent
joint
disease
affecting
many
people
globally,
presents
significant
challenge
in
current
treatments,
which
often
only
manage
symptoms
without
halting
progression.
This
review
illuminates
novel
approaches
OA
therapy,
focusing
mainly
on
intra‐articular
injection
of
nanomaterials.
The
innovative
materials,
designed
to
either
mimic
or
augment
natural
lubrication,
show
promise
restoring
biomechanics
and
alleviating
pain.
delves
into
an
array
biomimetic
lubricants,
including
polymer
brush,
nanocomposite
hydrogel,
nanoparticles,
underscoring
their
roles
anti‐inflammation,
targeting,
cartilage
repair,
drug
delivery.
Furthermore,
the
potential
mesenchymal
stem
cells
differentiate
chondrocytes,
coupled
with
delivery
these
exosomes
via
nanomaterials,
has
promoted
cell
therapy
avenues
for
OA.
also
highlights
function
non‐coding
RNAs
such
as
miRNA,
siRNA,
circRNA,
lncRNA,
antisense
oligonucleotides
impeding
progression,
nanomaterials
facilitating
delivery,
thus
advanced
therapeutic
possibilities
immune
evasion
bone
proliferation.
Overall,
this
encapsulates
evolution
treatment
from
material
cell,
ultimately
gene
forecasting
future
where
evolve
toward
integrated,
personalized
diagnostics
therapeutics
Journal of Advanced Research,
Год журнала:
2024,
Номер
66, С. 329 - 347
Опубликована: Янв. 11, 2024
Bone
metabolism
can
maintain
the
normal
homeostasis
and
function
of
bone
tissue.
Once
balance
is
broken,
it
will
cause
osteoporosis,
osteoarthritis,
defects,
tumors,
or
other
diseases.
However,
such
orthopedic
diseases
still
have
many
limitations
in
clinical
treatment,
as
drug
restrictions,
tolerance,
side
effects,
implant
rejection.
review:
In
complex
therapy
regeneration,
extracellular
derivatives
become
a
promising
research
focus
to
solve
problems
metabolic
These
derivatives,
which
include
components
matrix,
growth
factors,
vesicles,
significant
therapeutic
potential.
It
has
advantages
good
biocompatibility,
low
immune
response,
dynamic
demand
for
The
purpose
this
review
provide
comprehensive
perspective
on
elucidate
intrinsic
properties
versatility
derivatives.
Further
discussion
them
innovative
advanced
materials
improving
effectiveness
regeneration
processes.
Key
scientific
concepts
review,
we
first
listed
types
functions
three
Then,
discussed
effects
different
cell
sources
metabolism.
Subsequently,
collected
applications
treatment
summarized
challenges
applications.
Finally,
prospected
novel
We
hope
that
understanding
new
solutions
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 4, 2025
Abstract
Chondrocyte
senescence
is
an
important
pathogenic
factor
causing
osteoarthritis
(OA)
progression
through
persistently
producing
pro‐inflammatory
factors.
Mesenchymal
stem
cells‐derived
small
extracellular
vesicles
(MSC‐sEVs)
have
shown
anti‐inflammatory
effects
in
OA
models,
while
persistent
existence
of
senescent
chondrocytes
still
promotes
cartilage
destruction.
Therefore,
improving
the
targeted
elimination
ability
on
required
to
facilitate
translation
MSC‐sEVs
treatment.
In
this
study,
versatile
engineered
are
developed
targetedly
clear
and
maintain
metabolic
homeostasis.
Specifically,
loaded
with
siRNA
mouse
double
minute
2
homologue
(siMDM2)
modified
cartilage‐targeting
peptide
WYRGRL‐PEG
2K
‐DSPE
(WPD),
named
WPD‐sEVs
siMDM2
.
The
results
demonstrate
modification
improves
cellular
uptake
chondrocytes,
thus
antiaging
effects.
Importantly,
multifunctional
enhances
penetration
extends
joint
retention
time
MSC‐sEVs.
both
post‐traumatic
mice
naturally
aged
mice,
more
effectively
eliminates
maintained
matrix
By
using
P53
phosphorylation
inhibitor,
essential
role
MDM2‐P53
pathway
function
verified.
ex
vivo
cultured
human
explants,
it
confirmed
that
alleviates
phenotype.
Altogether,
findings
suggest
promising
translational
potential
for
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 23, 2025
Articular
cartilage
injuries
can
lead
to
pain,
stiffness,
and
reduced
mobility,
may
eventually
progress
osteoarthritis
(OA).
Despite
substantial
research
efforts,
effective
therapies
capable
of
regenerating
are
still
lacking.
Mesenchymal
stromal
cells
(MSCs)
known
for
their
differentiation
immunomodulatory
capabilities,
yet
challenges
such
as
limited
survival
post-injection
inconsistent
therapeutic
outcomes
hinder
clinical
application.
Recent
evidence
suggests
that
the
beneficial
effects
MSCs
largely
mediated
by
secreted
small
extracellular
vesicles
(sEVs),
which
have
been
shown
promote
tissue
repair
reduce
inflammation.
MSC-derived
sEVs
promise
in
mitigating
degradation
chondrocyte
apoptosis,
positioning
them
a
promising
alternative
MSC-based
OA
treatment.
This
review
explores
potential
limitations
regeneration.
systematic
was
conducted
following
PRISMA
guidelines,
with
comprehensive
search
Web
Science
Scopus
databases
studies
published
between
2019
2024.
A
total
223
records
were
identified,
132
articles
assessed
eligibility
based
on
general
selection
criteria.
After
full-text
screening,
60
initially
selected,
comprising
58
vitro
40
vivo
studies.
Following
further
exclusion
specific
criteria,
33
28
from
47
scientific
papers
included
final
qualitative
synthesis.
Most
indicate
enhance
proliferation,
improve
matrix
composition,
matrix-degrading
enzymes
inflammation,
thereby
delaying
progression.
growing
body
supports
use
tool
preventing
progression,
most
reporting
structure
function.
However,
remain
optimizing
standardizing
isolation,
dosage,
delivery
methods
Further
is
necessary
elucidate
mechanisms
underlying
sEVs-mediated
regeneration
facilitate
translation
into
therapies.
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Фев. 12, 2025
Bone-related
diseases
impact
a
large
portion
of
the
global
population
and,
due
to
their
high
disability
rates
and
limited
treatment
options,
pose
significant
medical
economic
challenges.
Mesenchymal
stem
cells
(MSCs)
can
differentiate
into
multiple
cell
types
offer
strong
regenerative
potential,
making
them
promising
for
treating
various
diseases.
However,
issues
with
immune
response
survival
limit
effectiveness
transplantation.
This
has
led
increased
interest
in
cell-free
therapy,
particularly
use
exosomes,
which
is
most
studied
form
this
approach.
Exosomes
are
extracellular
vesicles
that
contain
proteins,
lipids,
nucleic
acids
play
key
role
communication
material
exchange.
Pyroptosis,
death
involved
innate
immunity,
also
associated
many
Studies
have
shown
MSC-derived
exosomes
therapeutic
potential
range
conditions
by
regulating
inflammation
pyroptosis.
study
explored
modulating
pyroptosis
improve
bone-related
Biofabrication,
Год журнала:
2023,
Номер
16(1), С. 015008 - 015008
Опубликована: Окт. 19, 2023
Abstract
The
integrated
repair
of
cartilage
and
bone
involves
the
migration
differentiation
cells,
which
has
always
been
a
difficult
problem
to
be
solved.
We
utilize
natural
biomaterial
gelatin
construct
methacryloyl
(GelMA),
hydrogel
scaffold
with
high
cell
affinity.
GelMA
is
mixed
different
components
print
bi-layer
porous
modulus
composition
in
upper
lower
layers
through
three-dimensional
(3D)
printing
technology.
adds
black
phosphorus
(BP)
human
umbilical
cord
mesenchymal
stem
cells
(hUMSCs)
exosomes
(exos)
GelMA,
relatively
elastic
conducive
BMSCs
into
cartilage.
In
scaffold,
addition
BP
hUMSCs
exos,
β
-tricalcium
phosphate
(
-TCP),
osteoconductive
osteoinductive
effects,
added
GelMA.
-TCP
significantly
enhances
osteogenic
marrow
cells(BMSCs).
vitro
experiments
have
confirmed
that
scaffolds
can
promote
osteogenesis
chondrogenic
respectively.
And
rabbit
cartilage–bone
injury
model,
MRI
micro-CT
results
show
3D
printed
composite
effect
close
normal
tissue.
Journal of Advanced Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 1, 2024
As
people
age,
degenerative
bone
and
joint
diseases
(DBJDs)
become
more
prevalent.
When
middle-aged
elderly
are
diagnosed
with
one
or
disorders
such
as
osteoporosis
(OP),
osteoarthritis
(OA),
intervertebral
disc
degeneration
(IVDD),
it
often
signals
the
onset
of
prolonged
pain
reduced
functionality.
Chronic
inflammation
has
been
identified
underlying
cause
various
diseases,
including
DBJDs.
Recently,
excessive
activation
pyroptosis,
a
form
programed
cell
death
(PCD)
mediated
by
inflammasomes,
emerged
primary
driver
harmful
chronic
inflammation.
Consequently,
pyroptosis
potential
target
for
preventing
treating
This
review
explored
physiological
pathological
roles
pathway
in
development
its
relation
to
Meanwhile,
elaborated
molecular
mechanisms
within
individual
types
marrow
joints,
well
interplay
among
different
context
Furthermore,
this
presented
latest
compelling
evidence
supporting
idea
regulating
DBJDs
treatment,
discussed
potential,
limitations,
challenges
therapeutic
strategies
involving
regulation.
In
summary,
an
interesting
identity
unregulated
was
proposed
review,
which
undertaken
spoiler
peaceful
coexistence
between
cells
environment.
Over
extended
course
DBJDs,
perpetuated
activity
through
crosstalk
cascades
types,
thus
exacerbating
inflammatory
environment
throughout
entire
Correspondingly,
regulation
therapy
promising
option
clinical
treatment
