Materials Today Bio,
Год журнала:
2024,
Номер
26, С. 101104 - 101104
Опубликована: Май 27, 2024
Clinical
treatment
of
diabetic
refractory
ulcers
is
impeded
by
chronic
inflammation
and
cell
dysfunction
associated
with
wound
healing.
The
significant
clinical
application
bFGF
in
healing
limited
its
instability
vivo.
Sulfur
has
been
applied
for
the
skin
diseases
clinic
antibiosis.
We
previously
found
that
sulfur
incorporation
improves
ability
selenium
nanoparticles
to
accelerate
healing,
yet
toxicity
still
poses
a
risk
application.
To
obtain
materials
high
pro-regeneration
activity
low
toxicity,
we
explored
mechanism
which
selenium-sulfur
aid
via
RNA-Seq
designed
nanoparticle
called
Nano-S@bFGF,
was
constructed
from
bFGF.
As
expected,
Nano-S@bFGF
not
only
regenerated
zebrafish
tail
fins
promoted
but
also
repair
mice
profitable
safety
profile.
Mechanistically,
successfully
coactivated
FGFR
Hippo
signalling
pathways
regulate
Briefly,
reported
here
provides
an
efficient
feasible
method
synthesis
bioactive
nanosulfur
In
long
term,
our
results
reinvigorated
efforts
discover
more
peculiar
unique
biofunctions
great
variety
human
diseases.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Апрель 23, 2024
Abstract
Wound
healing
is
an
obvious
clinical
concern
that
can
be
hindered
by
inadequate
angiogenesis,
inflammation,
and
chronic
hypoxia.
While
exosomes
derived
from
adipose
tissue-derived
stem
cells
have
shown
promise
in
accelerating
carrying
therapeutic
growth
factors
microRNAs,
intracellular
cargo
delivery
compromised
hypoxic
tissues
due
to
activated
hypoxia-induced
endocytic
recycling.
To
address
this
challenge,
we
developed
a
strategy
coat
oxygen
nanobubbles
with
incorporate
them
into
polyvinyl
alcohol/gelatin
hybrid
hydrogel.
This
approach
not
only
alleviates
wound
hypoxia
but
also
offers
efficient
means
of
delivering
exosome-coated
nanoparticles
conditions.
The
self-healing
properties
the
hydrogel,
along
its
component,
gelatin,
aids
hemostasis,
while
crosslinking
bonds
facilitate
hydrogen
peroxide
decomposition,
ameliorate
inflammation.
Here,
show
potential
multifunctional
hydrogel
for
enhanced
healing,
promoting
facilitating
exosome
delivery,
mitigating
hypoxia,
inhibiting
inflammation
male
rat
full-thickness
model.
Biomedicines,
Год журнала:
2024,
Номер
12(4), С. 743 - 743
Опубликована: Март 27, 2024
Mesenchymal
stem
cells
(MSCs)
have
been
recognized
as
a
cell
therapy
with
the
potential
to
promote
skin
healing.
MSCs,
their
multipotent
differentiation
ability,
can
generate
various
related
wound
healing,
such
dermal
fibroblasts
(DFs),
endothelial
cells,
and
keratinocytes.
In
addition,
MSCs
neovascularization,
cellular
regeneration,
tissue
healing
through
mechanisms
including
paracrine
autocrine
signaling.
Due
these
characteristics,
extensively
studied
in
context
of
burn
chronic
repair.
Furthermore,
during
investigation
unique
roles
aging
scarless
also
discovered.
this
review,
we
summarize
by
which
discuss
recent
findings
from
preclinical
clinical
studies.
We
explore
strategies
enhance
therapeutic
effects
MSCs.
Moreover,
emerging
trend
combining
engineering
techniques,
leveraging
advantages
materials,
biodegradable
scaffolds
hydrogels,
repair
capacity
Additionally,
highlight
using
characteristics
cell-free
therapies
future
direction
cell-based
treatments,
further
demonstrating
regenerative
aesthetic
applications
regeneration.
Bioactive Materials,
Год журнала:
2024,
Номер
40, С. 597 - 623
Опубликована: Авг. 15, 2024
Tissue
engineering
technology
has
advanced
rapidly
in
recent
years,
offering
opportunities
to
construct
biologically
active
tissues
or
organ
substitutes
repair
even
enhance
the
functions
of
diseased
and
organs.
Tissue-engineered
scaffolds
rebuild
extracellular
microenvironment
by
mimicking
matrix.
Fibrin-based
possess
numerous
advantages,
including
hemostasis,
high
biocompatibility,
good
degradability.
Fibrin
provide
an
initial
matrix
that
facilitates
cell
migration,
differentiation,
proliferation,
adhesion,
also
play
a
critical
role
cell-matrix
interactions.
are
now
widely
recognized
as
key
component
tissue
engineering,
where
they
can
facilitate
defect
repair.
This
review
introduces
properties
fibrin,
its
composition,
structure,
biology.
In
addition,
modification
cross-linking
modes
fibrin
discussed,
along
with
various
forms
commonly
used
engineering.
We
describe
biofunctionalization
fibrin.
provides
detailed
overview
use
applications
skin,
bone,
nervous
tissues,
novel
insights
into
future
research
directions
for
clinical
treatment.
Giant,
Год журнала:
2024,
Номер
18, С. 100251 - 100251
Опубликована: Март 6, 2024
The
treatment
of
diabetic
wounds
is
a
major
challenge
faced
by
the
medical
system,
and
there
growing
interest
in
developing
innovative
therapies
to
accelerate
wound
healing.
Regenerative
medicine
with
cells
has
shown
promising
potential
skin
repair,
regenerative
properties
primarily
attributed
paracrine
effects
secreted
products,
including
exosomes.
Compared
cell-based
approaches,
using
exosomes
as
cell-free
therapy
for
chronic
several
advantages.
Exosomes
can
regulate
intercellular
communication
releasing
their
contents,
mRNA,
miRNA,
lipids,
proteins,
which
further
promote
are
well
explored
biomedical
application
owing
advantages
such
biocompatibility
low
immunogenicity.
However,
common
method
exosome
administration
through
injection,
but
due
rapid
clearance
rate
body,
maintaining
necessary
therapeutic
concentration
around
challenging.
Therefore,
it
develop
new
biocompatible
scaffold
carrier
extracellular
vesicles,
allowing
them
sustain
at
non-healing
sites
continuously
Engineered
kinds
modified
internal
treated
molecules,
surface
decoration
or
delivered
engineered
platform.
In
addition,
some
researchers
have
processed
exosomes,
known
decoration,
delivery
platforms.
regular
greater
promoting
this
review,
we
summarize
molecular
mechanisms
from
different
sources
varying
modifications
Advantages
limitations
repair
were
also
discussed.
Finally,
highlight
challenges
future
development
directions
translating
our
knowledge
into
clinical
practice.
Cell Biology and Toxicology,
Год журнала:
2025,
Номер
41(1)
Опубликована: Янв. 7, 2025
Vascular
endothelial
cell-derived
exosomes
are
thought
to
mediate
disease
progression
by
regulating
macrophage
polarization.
However,
its
mechanism
in
diabetes
mellitus
(DM)-related
atherosclerosis
(AS)
progress
is
unclear.
High-glucose
(HG)
and
oxLDL
were
used
induce
human
cardiac
microvascular
cells
(HCMECs)
mimic
DM-related
AS
model.
The
conditioned
medium
(CM)
from
HG+oxLDL-induced
HCMECs
was
incubated
with
THP1-M0
monocytes
treated
LPS
or
oxLDL.
mRNA
levels
of
M1/M2
polarization
markers,
NEDD4L,
IκBα
PPARγ
determined
qRT-PCR.
Flow
cytometry
analyze
marker.
Dil-labeled
oil
red
O
staining
performed
assess
uptake
cells.
inflammatory
factors
examined
using
ELISA.
Transmission
electron
microscope
for
observing
foam
cell
formation
exosome
morphology.
protein
p-Smad1/Smad1,
p-Smad2/Smad2,
p-IκBα/IκBα,
p-P65/P65,
anti-lipid
metabolism-related
NEDD4L
tested
western
blot.
interaction
between
assessed
Co-IP
assay.
CM
could
promote
M1
polarization,
formation,
inhibiter
GW4869
eliminated
these
effects.
overexpressed
HCMECs,
which
be
taken
up
Exosomal
knockdown
inhibited
reducing
the
p-IκBα/IκBα
p-P65/P65.
reduce
expression
through
ubiquitination.
HCMECs-derived
exosomal
enhance
ubiquitination
facilitate
uptake,
thus
accelerating
AS.
