Endplate chondrocyte-derived exosomal miR-128-3p mitigates intervertebral disc degeneration by targeting TRAF6 via the miR-128-3p/TRAF6 axis to suppress pyroptosis DOI Creative Commons
Qiuwei Li,

Ruocheng Guo,

Zuomeng Wu

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113620 - 113620

Опубликована: Ноя. 16, 2024

Intervertebral disc degeneration (IVDD) is a leading cause of chronic back pain and significantly impacts quality life. The pathogenesis IVDD largely driven by inflammation, pyroptosis, extracellular matrix (ECM) degradation, which current therapies fail to adequately address. In this study, we explore the therapeutic potential exosomes derived from endplate chondrocytes (EPCs), with particular focus on microRNA miR-128-3p. Our findings reveal that isolated third-generation EPCs, enriched miR-128-3p, exhibit potent anti-inflammatory anti-pyroptotic effects in lipopolysaccharide-treated nucleus pulposus cells, are key contributors pathology. Specifically, demonstrate miR-128-3p delivered via EPC-derived directly targets TRAF6, effectively suppressing activation NF-κB signaling pathway, known play pivotal role inflammation ECM breakdown, marked reduction pro-inflammatory cytokine release mitigation degradation. Importantly, EPC exosomes, higher levels showed superior efficacy compared fifth-generation underscoring critical mediating these protective effects. research highlights promise particularly those rich as novel, cell-free approach for IVDD. Unlike treatments primarily symptom management, our molecular pathways underlying progression, including By elucidating miR-128-3p/TRAF6 axis, study provides foundation development targeted, biologically based interventions aimed at halting or even reversing IVDD, thereby offering hope more effective lasting options.

Язык: Английский

Innovating intervertebral disc degeneration therapy: Harnessing the power of extracellular vesicles DOI Creative Commons
Shanfeng Chen,

Yiming Dou,

Yiming Zhang

и другие.

Journal of Orthopaedic Translation, Год журнала: 2025, Номер 50, С. 44 - 55

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

Dissolving Microneedles as In Situ Chemical Reaction Chambers: from Design Strategies to Versatile Biomedical Applications DOI Open Access
Yu Tian, Lili Xia, Xinran Song

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

Abstract The skin microenvironment is a highly intricate and dynamic system, characterized by an acidic pH, diverse microbiota, various metabolites, numerous enzymes, creating both challenges opportunities for the development of innovative drug delivery systems. Dissolving Microneedles (MNs) have emerged as promising, pain‐free alternative to conventional invasive injections, offering ability deliver therapeutics through gradual degradation within skin's interstitial fluids. Building upon unique properties dissolving MNs, novel concept introduced wherein MNs serve in situ chemical reaction chambers. In this framework, can reactants or catalysts skin, enabling initiation specific reactions, such prodrug activation targeted therapy, harmful enhanced synthesis beneficial molecules. Moreover, review systematically explores potential chambers, discussing key aspects their sustained release mechanisms, design strategies, range therapeutic applications. Finally, forward‐looking perspective provided on future addressing broader clinical translation application personalized medicine.

Язык: Английский

Процитировано

0

Cutting-Edge Biomaterials in Intervertebral Disc Degeneration Tissue Engineering DOI Creative Commons
Yifan Wang, Chuyue Zhang,

Junyao Cheng

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(8), С. 979 - 979

Опубликована: Июль 24, 2024

Intervertebral disc degeneration (IVDD) stands as the foremost contributor to low back pain (LBP), imposing a substantial weight on world economy. Traditional treatment modalities encompass both conservative approaches and surgical interventions; however, former falls short in halting IVDD progression, while latter carries inherent risks. Hence, quest for an efficacious method reverse onset is paramount. Biomaterial delivery systems, exemplified by hydrogels, microspheres, microneedles, renowned their exceptional biocompatibility, biodegradability, biological efficacy, mechanical attributes, have found widespread application bone, cartilage, various tissue engineering endeavors. Consequently, IVD has emerged burgeoning field of interest. This paper succinctly introduces intervertebral (IVD) structure pathophysiology IVDD, meticulously classifies biomaterials repair, reviews recent advances field. Particularly, strengths weaknesses are emphasized, potential avenues future research suggested.

Язык: Английский

Процитировано

3

Responsive porous microneedles with riboflavin ocular microinjection capability for facilitating corneal crosslinking DOI Creative Commons
Xiaojun Hu, Bin Kong, Yunzhe Wang

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Сен. 28, 2024

Язык: Английский

Процитировано

3

The pathogenesis and targeted therapies of intervertebral disc degeneration induced by cartilage endplate inflammation DOI Creative Commons
Haiyan Yang, Xiaoyan Chen, Jun Chen

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Дек. 2, 2024

Intervertebral disc degeneration (IVDD) is the leading cause of low back pain, where and death nucleus pulposus cells within intervertebral (IVD) can be obviously revealed. This result in an imbalance extracellular matrix due to loss proteoglycans water content, which further lead catabolic anabolic dysfunction IVD. Recently, cartilage endplate (CEP) during aging has drawn large attention its essential functions contributing nutrient exchange maintaining IVD homeostasis. Furthermore, inflammation disturbed homeostasis CEP not only accelerate degradation matrix, but also exacerbate IVDD by causing cell through other pathological factors. Here this review, we summarized possible factors underlying mechanisms inflammation-induced IVDD, including exosomes degeneration, calcification, ferroptosis, mechanical changes, senescence. Besides, changes miRNAs, pain-related neural reflex arc pathways associated with are reviewed. In addition, new strategies specifically designed for discussed last section. We hope paper offer some insights advancing novel treating serve as a valuable reference researchers field.

Язык: Английский

Процитировано

2

Endplate chondrocyte-derived exosomal miR-128-3p mitigates intervertebral disc degeneration by targeting TRAF6 via the miR-128-3p/TRAF6 axis to suppress pyroptosis DOI Creative Commons
Qiuwei Li,

Ruocheng Guo,

Zuomeng Wu

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113620 - 113620

Опубликована: Ноя. 16, 2024

Intervertebral disc degeneration (IVDD) is a leading cause of chronic back pain and significantly impacts quality life. The pathogenesis IVDD largely driven by inflammation, pyroptosis, extracellular matrix (ECM) degradation, which current therapies fail to adequately address. In this study, we explore the therapeutic potential exosomes derived from endplate chondrocytes (EPCs), with particular focus on microRNA miR-128-3p. Our findings reveal that isolated third-generation EPCs, enriched miR-128-3p, exhibit potent anti-inflammatory anti-pyroptotic effects in lipopolysaccharide-treated nucleus pulposus cells, are key contributors pathology. Specifically, demonstrate miR-128-3p delivered via EPC-derived directly targets TRAF6, effectively suppressing activation NF-κB signaling pathway, known play pivotal role inflammation ECM breakdown, marked reduction pro-inflammatory cytokine release mitigation degradation. Importantly, EPC exosomes, higher levels showed superior efficacy compared fifth-generation underscoring critical mediating these protective effects. research highlights promise particularly those rich as novel, cell-free approach for IVDD. Unlike treatments primarily symptom management, our molecular pathways underlying progression, including By elucidating miR-128-3p/TRAF6 axis, study provides foundation development targeted, biologically based interventions aimed at halting or even reversing IVDD, thereby offering hope more effective lasting options.

Язык: Английский

Процитировано

0