Mesoporous TiO₂ Coatings Regulate ZnO Nanoparticle Loading and Zn²⁺ Release on Titanium Dental Implants for Sustained Osteogenic and Antibacterial Activity

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(12), С. 15235 - 15249

Опубликована: Март 17, 2023

Two major issues are currently hindering the clinical practice of titanium dental implants for lack biological activities: immediate/early loading risks and peri-implantitis. To solve these issues, it is urgent to develop multifunctional modified with effective osteogenic antibacterial properties. Zinc oxide nanoparticles (ZnO NPs) possess superior activity; however, they can rapidly release Zn2+, causing cytotoxicity. In this study, a potential implant modification was creatively developed as ZnO nanoparticle-loaded mesoporous TiO2 coatings (nZnO/MTC-Ti) via evaporation-induced self-assembly method (EISA) one-step spin coating. The (MTCs) regulated synthesis NPs inside nanosized pores. synergistic effects MTC on nZnO/MTC-Ti not only controlled long-term steady-state Zn2+ but also optimized charge distribution surface. Therefore, cytotoxicity resolved without triggering excessive reactive oxygen species (ROS). increased extracellular further promoted favorable intracellular zinc ion microenvironment through modulation transporters (ZIP1 ZnT1). Owing that, adhesion, proliferation, activity bone mesenchymal stem cells (BMSCs) were improved. Additionally, inhibited proliferation oral pathogens (Pg Aa) by inducing bacterial ROS production. For in vivo experiments, different implanted into alveolar fossa Sprague–Dawley rats immediately after tooth extraction. found higher capability enhancing regeneration, antibiosis, osseointegration vivo. These findings suggested outstanding performance accelerating inhibiting infection, indicating huge solving peri-implantitis implants.

Язык: Английский

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Advanced strategies of scaffolds design for bone regeneration

BMEMat, Год журнала: 2023, Номер 1(4)

Опубликована: Сен. 2, 2023

Abstract Bone defects are encountered substantially in clinical practice, and bionic scaffolds represent a promising solution for repairing bone defects. However, it is difficult to fabricate with structures reconstruct the microenvironment fulfill satisfying repair effects. In this review article, we first discuss various strategies design construction of promote defect repair, especially including structural scaffold integration bioactive substances together application external stimuli. We then roles artificial intelligence medical imaging aiding treatment. Finally, point out challenges future outlooks developing multifunctional scaffolds, aiming provide insights improving regeneration efficacy accelerating translation.

Язык: Английский

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Piezoelectric stimulation enhances bone regeneration in alveolar bone defects through metabolic reprogramming of macrophages

Exploration, Год журнала: 2024, Номер 4(6)

Опубликована: Июнь 10, 2024

Abstract Immunomodulation has emerged as a promising strategy for promoting bone regeneration. However, designing osteoimmunomodulatory biomaterial that can respond to mechanical stress in the unique microenvironment of alveolar under continuous occlusal remains significant challenge. Herein, wireless piezoelectric stimulation system, namely, hydrogel incorporating BaTiO 3 nanoparticles (BTO NPs), is successfully developed generate potentials modulating macrophage reprogramming. The reprograms macrophages towards M2 phenotype, which subsequently induces osteogenic differentiation marrow mesenchymal stem cells (BMSCs). RNA sequencing analysis reveals piezoelectricity‐modulated polarization closely associated with metabolic reprogramming, including increased amino acid biosynthesis and fatty oxidation. composite excellent biocompatibility exhibits immunomodulatory osteoinductive activities. In rat model defects, effectively promotes endogenous regeneration at load‐bearing sites. piezoelectric‐driven osteoimmunomodulation proposed this study not only broadens understanding mechanism underlying biomaterials tissue but also provides new insights into design development next‐generation biomaterials.

Язык: Английский

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Regulation of Macrophage Polarization Through Periodic Photo‐Thermal Treatment to Facilitate Osteogenesis

Small, Год журнала: 2022, Номер 18(38)

Опубликована: Авг. 19, 2022

Abstract The richened reactive oxygen species (ROS) and their derived excessive inflammation at bone injured sites hinder osteogenesis of endosseous Ti‐based implants. Herein, anti‐oxidized polydopamine (PDA) is deposited on hydrothermal growth formed hydroxyapatite (HA) nanorods Ti to form a core‐shell structural nanorod‐like array with HA as core PDA an amorphous shell (PDA@HA), showing not only ROS scavenging ability but also near‐infrared (NIR) light photo‐thermal effects. PDA@HA suppresses based its certain extent, while periodic treatment (PTT) mild temperature (41 ± 1 °C) further accelerates the transition macrophages (MΦs) adhered from pro‐inflammatory (M1) phenotype anti‐inflammatory (M2) in vitro vivo. Transcriptomic analysis reveals that activation PI3K‐Akt1 signaling pathway responsible for PTT induced acceleration M1‐to‐M2 MΦs. Acting mesenchymal stem cells (MSCs) paracrine cytokines M2 macrophages, greatly promote osteogenetic functions MSCs thus osteogenesis. This work paves way employing mildly induce favorable immunomodulatory microenvironment provides insights into underlying immunomodulation mechanism.

Язык: Английский

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High entropy alloy coatings for biomedical applications: A review

Smart Materials in Manufacturing, Год журнала: 2022, Номер 1, С. 100009 - 100009

Опубликована: Сен. 28, 2022

Metallic biomaterials are widely used as short and long-term implantable devices by virtue of their outstanding mechanical properties, such high load-bearing capacity fatigue resistance. Due to inherent bioinertness, potential corrosion, some inferior surface metallic generally require coating modification improve function extend lifespan in the body. High entropy alloys (HEAs) a novel class materials that composed at least five principal elements with equiatomic or close-to-equiatomic compositions. Some unique properties HEAs for include excellent corrosion resistance, remarkable wear strength/hardness, strong diffusion The on substrates can be achieved through different techniques, including thermal spraying, laser deposition, vapor deposition. have become promising candidate biomedical applications combining tailor-made topography, biocompatibility, appropriate chemistry, element composition design. present article is thorough review research using HEAs.

Язык: Английский

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Phosphatidylserine liposome multilayers mediate the M1-to-M2 macrophage polarization to enhance bone tissue regeneration

Acta Biomaterialia, Год журнала: 2022, Номер 154, С. 583 - 596

Опубликована: Окт. 21, 2022

Язык: Английский

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Application of Bioactive Materials for Osteogenic Function in Bone Tissue Engineering

Small Methods, Год журнала: 2024, Номер 8(8)

Опубликована: Март 21, 2024

Bone tissue defects present a major challenge in orthopedic surgery. engineering using multiple versatile bioactive materials is potential strategy for bone-defect repair and regeneration. Due to their unique physicochemical mechanical properties, biofunctional can enhance cellular adhesion, proliferation, osteogenic differentiation, thereby supporting stimulating the formation of new bone tissue. 3D bioprinting physical stimuli-responsive strategies have been employed various studies on regeneration fabrication desired multifunctional biomaterials with integrated properties. In this review, applied engineering, emerging techniques, rational manufacturing novel therapeutic regenerative functions are summarized. Furthermore, impact differentiation stem cells pathways associated biomaterial-induced osteogenesis discussed.

Язык: Английский

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Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(17)

Опубликована: Янв. 13, 2024

Abstract Biomaterials are defined as “engineered materials” and include a range of natural synthetic products, designed for their introduction into interaction with living tissues. considered prominent tools in regenerative medicine that support the restoration tissue defects retain physiologic functionality. Although commonly used medical field, these constructs inherently foreign toward host induce an immune response at material–tissue interface, body (FBR). A strong connection between regeneration is suggested, which appropriate amount macrophage polarization necessary to trigger autologous formation. Recent developments this field have led characterization immunomodulatory traits optimizes bioactivity, integration biomaterials determines fate regeneration. This review addresses variety aspects involved steering inflammatory response, including cell interactions, physical characteristics, biochemical cues, metabolomics. Harnessing advancing knowledge FBR allows optimization biomaterial‐based implants, aiming prevent damage implant, improve regeneration, provide efficient successful vivo implantation.

Язык: Английский

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Metformin Facilitates Osteoblastic Differentiation and M2 Macrophage Polarization by PI3K/AKT/mTOR Pathway in Human Umbilical Cord Mesenchymal Stem Cells

Stem Cells International, Год журнала: 2022, Номер 2022, С. 1 - 12

Опубликована: Июнь 18, 2022

Mesenchymal stem cells (MSCs) are the most promising multipotent that can differentiate into osteoblasts, chondrocytes, and adipocytes. This cellular flexibility contributes to widespread clinical use of MSCs in tissue repair regeneration. The immune system is a key player regulating bone remodeling. In recent years, association between metabolism has become an increasing focus interest. Metformin, glucose-lowering drug, exerts powerful impact on metabolic signaling. However, whether metformin modulate or influence milieu by regulation macrophages not been thoroughly elucidated. Herein, we specifically explored complex interactions human umbilical cord mesenchymal (UC-MSCs) context metformin. Our research demonstrated only stimulated osteogenesis UC-MSCs but also influenced via promoting M2 reducing M1 macrophages. Mechanically, found metformin-treated possessed more potent osteoinductive capacity our coculture system. Molecularly, these metformin-stimulated facilitated activating PI3K/AKT/mTOR pathway. As using PI3K-specific inhibitor LY294002, pathway partly reversed activity which was activated Overall, novel illuminated cooperative synergistic effects dynamic balance metabolism.

Язык: Английский

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Intrafibrillar Mineralization and Immunomodulatory for Synergetic Enhancement of Bone Regeneration via Calcium Phosphate Nanocluster Scaffold

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(12)

Опубликована: Март 3, 2023

Abstract Inspired by the bionic mineralization theory, organic–inorganic composites with hydroxyapatite nanorods orderly arranged along collagen fibrils have attracted extensive attention. Planted an ideal bone scaffold will contribute greatly to osteogenic microenvironment; however, it remains challenging develop a biomimetic ability promote intrafibrillar and simultaneous regulation of immune microenvironment in situ. To overcome these challenges, containing ultra‐small particle size calcium phosphate nanocluster (UsCCP) is prepared, which can enhance regeneration through synergetic effect immunomodulatory. By efficient infiltration into fibrils, UsCCP released from achieves mineralization. It also promotes M2‐type polarization macrophages, leading both angiogenic potential. The results confirm that has immunomodulatory effects, making promising candidate for regeneration.

Язык: Английский

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