Monitoring Autophagy in Human Aging: Key Cell Models and Insights

Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(3)

Опубликована: Март 20, 2025

Autophagy, a key cellular degradation and recycling pathway, is critical for maintaining homeostasis responding to metabolic environmental stress. Evidence age-related autophagic dysfunction its implications in chronic diseases including neurodegeneration accumulating. However, as complex, multi-step process, autophagy can be challenging measure, particularly humans human aging- disease-relevant models. This review describes the links between macroautophagy, aging, diseases. We present three novel cell models, peripheral blood mononuclear cells (PBMCs), primary dermal fibroblasts (PDFs), induced neurons (iNs), which serve essential tools studying flux assessing potential biomarker aging. Unlike traditional these models retain age- disease-associated molecular signatures, enhancing their relevance studies. The development of robust methodologies measuring holds promise advancing our understanding autophagy’s role aging diseases, ultimately facilitating discovery therapies enhance health outcomes.

Язык: Английский

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Deciphering the relationship between sarcopenia and aging: A combined text mining and bioinformatics approach

Geriatrics and gerontology international/Geriatrics & gerontology international, Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Aim Sarcopenia is recognized as an age‐related muscle disease, but there has been no comprehensive analysis of what different between normal aging and sarcopenia, with awareness the worldwide research to date. Therefore, in this study, we used text mining PubMed articles on sarcopenia focused our bioinformatics items that have identified. Methods This study compared gene‐level changes identify sarcopenia‐specific gene using high‐throughput sequencing data. In particular, was pathways mechanisms interest research, focus more these mechanisms. Results We identified common aging. Interleukin‐7 were associated both conditions. Although phagosome‐related suggested sarcopenia‐specific, significant phagosome formation, lysosome‐related mitophagy‐related groups However, genes nicotinamide adenine dinucleotide phosphate oxidase catalytic subunit family shown be possibly altered, suggesting involvement oxidative stress regulatory pathways. Conclusions A analysis, complemented by extant literature, might not characterized a failure autophagy whole, rather, disruption regulation, particularly subunit‐related at subsequent stage after phagosome‐lysosome fusion. Geriatr Gerontol Int 2025; ••: ••–•• .

Язык: Английский

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Harnessing immunomodulation to combat sarcopenia: current insights and possible approaches

Immunity & Ageing, Год журнала: 2024, Номер 21(1)

Опубликована: Авг. 5, 2024

Sarcopenia is a complex age-associated syndrome of progressive loss muscle mass and strength. Although this condition influenced by many factors, age-related changes in immune function including cell dynamics, chronic inflammation contribute to its progression. The interplay between the system, gut-muscle axis, autophagy further underscores their important roles sarcopenia pathogenesis. Immunomodulation has emerged as promising strategy counteract sarcopenia. Traditional management approaches treat physical exercise nutritional supplementation, emerging technologies biophysical stimulation demonstrated importance immunomodulation regulation macrophages T cells reduction inflammation. Treatments alleviate low-grade older adults modulating gut microbial composition diversity combat Furthermore, some pharmacological interventions, nano-medicine, therapies targeting muscle, microbiota, or present additional avenues for This narrative review explores immunological underpinnings sarcopenia, elucidating relationship system during ageing. Additionally, discusses new areas such axis autophagy, which bridge health. Insights into current potential through modulation are provided, along with suggestions future research directions therapeutic strategies. We aim guide investigation clinical biomarkers identify indicators diagnosis treatment targets condition. also draw attention considering

Язык: Английский

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Scientific landscape of oxidative stress in sarcopenia: from bibliometric analysis to hotspots review

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Ноя. 11, 2024

Sarcopenia is a significant healthcare challenge in the aging population. Oxidative stress (OS) acknowledged to play pivotal role pathological progression of sarcopenia. Numerous studies have demonstrated that mitigating or eliminating OS can ameliorate manifestations associated with However, current clinical antioxidant therapies often fall short anticipated outcomes. This bibliometric analysis aims delineate prevailing research trends, thematic emphases, focal points, and developmental trajectories within domain sarcopenia, while also endeavoring explore prospective anti-oxidative strategies for future interventions.

Язык: Английский

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Effects of upper extremity blood flow restriction training on muscle strength and hypertrophy: a systematic review and meta-analysis

Frontiers in Physiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 6, 2025

Low load resistance training with blood flow restriction (LL-BFRT) has been shown to improve muscle strength and hypertrophic function. The effect of LL-BFRT on lower extremity improvement widely discussed. However, no studies have discussed the this method upper muscles until now. This systematic review meta-analysis focused use in muscles. relevant literature was searched four major databases including Pubmed, Web science, Cochrane Library Embase from 10 June 2024. Collaboration's tool GRADE methodology were used assess risk bias quality included studies. a total 11 articles 220 participants. high (HLRT) produced similar effects improving (low certainty evidence, SMD: -0.35; 95%CI: -0.73 0.03; p: 0.07; I2: 2%) hypertrophy (moderate -0.36; 0.01; 0.05; 0%). Compared low (LLRT), showed greater advantages 0.67; 0.33 1.01; 0.0001; 0%) 0.37; 0.06 0.02; In general, can be as an alternative for HLRT hypertrophy. Our study shows that is limited by age region. It necessary formulate reasonable exercise programs according characteristics different demographic groups. https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42024555514.

Язык: Английский

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Effects of Bioconverted Guava Leaf (Psidium guajava L.) Extract on Skeletal Muscle Damage by Regulation of Ubiquitin–Proteasome System and Apoptosis in Type 2 Diabetic Mice

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3877 - 3877

Опубликована: Апрель 19, 2025

Skeletal muscle atrophy is one of the serious complications diabetes, which increases risk frailty, falls, and mortality. However, interventions for are limited, research needed regarding treatment wasting. Recently, bioconversion natural products by lactic acid bacteria has been highlighted as a possibility to improve bioavailability active ingredients. This process also produces metabolites, key signaling mediators variety physiological functions. study investigated effect bioconverted guava leaf (Psidium guajava L., GL) Lactobacillus plantarum on hyperglycemia-induced skeletal in type 2 diabetes mellites (T2DM) mice. Diabetes was induced high-fat diet with two-time streptozotocin (STZ) injection (60 mg/kg BW) male C57BL/6J After (a fasting blood glucose level (FBG) ≥ 300 mg/dL), mice were administered GL (100 mg/kg/day) or (FGL) (50 oral gavage 14 weeks. FGL contains different substances such hydroxyl-isocaproic hydroxyl-isovaleric compared GLE itself, have potential prevent degradation T2DM supplementation reduced FBG In addition, enhanced strength, cross-sectional area, ameliorated ubiquitin-proteasome system (UPS)-related pathways On other hand, tolerance demonstrated test insulin pathway. only attenuated inflammation apoptosis an improved mammalian target rapamycin (mTOR)-autophagy-related Although at half dose GLE, greater efficacy regulating expression these molecular markers. The result suggests that even itself anti-diabetic effects, functionality would be L. Plantarum, additive or/and synergistic effect. Taken together, could used nutraceutical attenuate inhibition inflammation, UPS,

Язык: Английский

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Exploration of Pathogenesis and Cutting-Edge Treatment Strategies of Sarcopenia: A Narrative Review

Clinical Interventions in Aging, Год журнала: 2025, Номер Volume 20, С. 659 - 684

Опубликована: Май 1, 2025

Sarcopenia a progressive and multifactorial musculoskeletal syndrome characterized by loss of muscle mass function, poses significant global health challenge, particularly in aging populations. Epidemiological studies reveal that sarcopenia affects approximately 5-10% the general population, with prevalence rates escalating dramatically after age 60 to reach 10-27% older adults. This age-associated increase contributes significantly healthcare burdens elevating risks disability, frailty, mortality. Despite its profound impact, current clinical approaches remain limited. While resistance exercise protein supplementation form cornerstone management, their efficacy is often constrained poor long-term adherence variable individual responses, highlighting urgent need for more comprehensive personalized treatment strategies. The pathogenesis complex influenced various factors, including aging, inflammation, nutritional deficits, physical inactivity, mitochondrial dysfunction. However, precise molecular mechanisms underlying this condition are still not fully understood. Recent research has made strides elucidating intricate contributing sarcopenia, revealing novel insights into cellular underpinnings. Notably, emerging evidence points pivotal role dysfunction, altered myokine profiles, neuromuscular junction degeneration progression. Additionally, breakthroughs stem cell therapy, exosome-based treatments, precision nutrition offer promising avenues intervention. review aims synthesize latest advancements research, focusing on contributions We explore trends such as senescence, epigenetic regulation, targeted therapeutic interventions could reshape future managing sarcopenia. By recent cutting-edge we hope advance understanding foster translation these findings effective therapies.

Язык: Английский

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Gender and age peculiarities of autophagy in men and women induced by a comprehensive wellness program: a prospective observational study

Kuban Scientific Medical Bulletin, Год журнала: 2024, Номер 31(2), С. 95 - 106

Опубликована: Апрель 28, 2024

Background . Autophagy refers to an evolutionary physiological mechanism of cell self-purification. The use its positive effects in regenerative and preventive medicine implies this process be investigated under the conditions various therapeutic factors. Objective To investigate influence gender, age somatic-morphological factors on autophagy processes induced by a comprehensive wellness program as expressed marker protein Beclin-1. Methods A prospective observational study involved 107 patients monitored before after procedures. All participants underwent clinic OOO Centr Zdorovie (Maykop, Russia). was conducted periods September‒December 2020 January 2021. measuring concentration Beclin-1 blood 12 days In order characterize degree activation process, index “delta-Beclin-1” introduced (difference between initial level registered days). response evaluated terms upward or downward direction (positive/negative delta- value, respectively). effect procedures functional systems organism determined accordance with laboratory tests (complete count, lipid profile), morphometric evaluation, assessment body composition impedance monitoring. obtained data were processed using SPSS Statistics 26.0 (IBM, USA). Results Monitoring revealed that basic activity is age-specific significantly lower individuals over 60 years compared both healthy persons. This correlation appears more pronounced obese (r = -0.59). men 30% than women, however, set mentioned increased 7%. found not all responded unidirectionally procedures: 49 out 77 showed increase concentration, while 28 — decrease. Patients who participated demonstrated negative expression age, those responding mass index, weight fat mass. source can refer mass, women lean especially mature period II (36‒60 years). Conclusion complex include gender weight. These obtain different significance at periods. serve alternative existing pharmacological methods for activating humans.

Язык: Английский

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Deciphering Osteosarcopenia through the hallmarks of aging

Mechanisms of Ageing and Development, Год журнала: 2024, Номер 222, С. 111997 - 111997

Опубликована: Окт. 11, 2024

Osteosarcopenia is a major driver of functional loss and risk factor for falls, fractures, disability mortality in older adults, urgently requiring the development effective interventions to address it. The hallmarks aging provide theoretical practical framework that allows structured organization current knowledge planning new lines. This article comprehensively reviews currently available literature on role osteosarcopenia, thereby offering panoramic view state art gaps this field.

Язык: Английский

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Irisin in degenerative musculoskeletal diseases: functions in system and potential in therapy

Pharmacological Research, Год журнала: 2024, Номер 210, С. 107480 - 107480

Опубликована: Окт. 25, 2024

Язык: Английский

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