Effect and mechanism of miRNA-144-5p-regulated autophagy in older adults with Sarcopenia

Immunity & Ageing, Journal Year: 2025, Volume and Issue: 22(1)

Published: Feb. 14, 2025

Advanced aging invariably triggers an overabundance of apoptosis, stemming from diminished autophagy or a disarray in cellular autophagic processes. This, turn, leads to accelerated breakdown muscle proteins, which exacerbates the ongoing deterioration skeletal and intensifies severity senile sarcopenia. This study aimed investigate role mechanism miRNA-regulated The miRNAs associated with sarcopenia were screened, target genes significant predicted. effects significantly differentially expressed miRNA-144-5p on cell validated vivo vitro. inhibition miR-144-5p enhanced multiplication mouse myoblasts, increased expression MHC markers LC3II/LC3I Beclin-1, facilitated formation autophagosomes reduced number cells senescence-related proteins acetylated p53, p21 myoblasts. affects myoblast senescence, myogenic differentiation, by regulating downstream gene, Atg2A. Inhibiting markedly grip strength posterior limb old mice, CSA mice young was also increased. All experiments have demonstrated that has impact regulation development

Language: Английский

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Monitoring Autophagy in Human Aging: Key Cell Models and Insights

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(3)

Published: March 20, 2025

Autophagy, a key cellular degradation and recycling pathway, is critical for maintaining homeostasis responding to metabolic environmental stress. Evidence age-related autophagic dysfunction its implications in chronic diseases including neurodegeneration accumulating. However, as complex, multi-step process, autophagy can be challenging measure, particularly humans human aging- disease-relevant models. This review describes the links between macroautophagy, aging, diseases. We present three novel cell models, peripheral blood mononuclear cells (PBMCs), primary dermal fibroblasts (PDFs), induced neurons (iNs), which serve essential tools studying flux assessing potential biomarker aging. Unlike traditional these models retain age- disease-associated molecular signatures, enhancing their relevance studies. The development of robust methodologies measuring holds promise advancing our understanding autophagy’s role aging diseases, ultimately facilitating discovery therapies enhance health outcomes.

Language: Английский

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Effects of Bioconverted Guava Leaf (Psidium guajava L.) Extract on Skeletal Muscle Damage by Regulation of Ubiquitin–Proteasome System and Apoptosis in Type 2 Diabetic Mice

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3877 - 3877

Published: April 19, 2025

Skeletal muscle atrophy is one of the serious complications diabetes, which increases risk frailty, falls, and mortality. However, interventions for are limited, research needed regarding treatment wasting. Recently, bioconversion natural products by lactic acid bacteria has been highlighted as a possibility to improve bioavailability active ingredients. This process also produces metabolites, key signaling mediators variety physiological functions. study investigated effect bioconverted guava leaf (Psidium guajava L., GL) Lactobacillus plantarum on hyperglycemia-induced skeletal in type 2 diabetes mellites (T2DM) mice. Diabetes was induced high-fat diet with two-time streptozotocin (STZ) injection (60 mg/kg BW) male C57BL/6J After (a fasting blood glucose level (FBG) ≥ 300 mg/dL), mice were administered GL (100 mg/kg/day) or (FGL) (50 oral gavage 14 weeks. FGL contains different substances such hydroxyl-isocaproic hydroxyl-isovaleric compared GLE itself, have potential prevent degradation T2DM supplementation reduced FBG In addition, enhanced strength, cross-sectional area, ameliorated ubiquitin-proteasome system (UPS)-related pathways On other hand, tolerance demonstrated test insulin pathway. only attenuated inflammation apoptosis an improved mammalian target rapamycin (mTOR)-autophagy-related Although at half dose GLE, greater efficacy regulating expression these molecular markers. The result suggests that even itself anti-diabetic effects, functionality would be L. Plantarum, additive or/and synergistic effect. Taken together, could used nutraceutical attenuate inhibition inflammation, UPS,

Language: Английский

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Deciphering the relationship between sarcopenia and aging: A combined text mining and bioinformatics approach

Geriatrics and gerontology international/Geriatrics & gerontology international, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Aim Sarcopenia is recognized as an age‐related muscle disease, but there has been no comprehensive analysis of what different between normal aging and sarcopenia, with awareness the worldwide research to date. Therefore, in this study, we used text mining PubMed articles on sarcopenia focused our bioinformatics items that have identified. Methods This study compared gene‐level changes identify sarcopenia‐specific gene using high‐throughput sequencing data. In particular, was pathways mechanisms interest research, focus more these mechanisms. Results We identified common aging. Interleukin‐7 were associated both conditions. Although phagosome‐related suggested sarcopenia‐specific, significant phagosome formation, lysosome‐related mitophagy‐related groups However, genes nicotinamide adenine dinucleotide phosphate oxidase catalytic subunit family shown be possibly altered, suggesting involvement oxidative stress regulatory pathways. Conclusions A analysis, complemented by extant literature, might not characterized a failure autophagy whole, rather, disruption regulation, particularly subunit‐related at subsequent stage after phagosome‐lysosome fusion. Geriatr Gerontol Int 2025; ••: ••–•• .

Language: Английский

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Scientific landscape of oxidative stress in sarcopenia: from bibliometric analysis to hotspots review

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Nov. 11, 2024

Sarcopenia is a significant healthcare challenge in the aging population. Oxidative stress (OS) acknowledged to play pivotal role pathological progression of sarcopenia. Numerous studies have demonstrated that mitigating or eliminating OS can ameliorate manifestations associated with However, current clinical antioxidant therapies often fall short anticipated outcomes. This bibliometric analysis aims delineate prevailing research trends, thematic emphases, focal points, and developmental trajectories within domain sarcopenia, while also endeavoring explore prospective anti-oxidative strategies for future interventions.

Language: Английский

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Gender and age peculiarities of autophagy in men and women induced by a comprehensive wellness program: a prospective observational study

Kuban Scientific Medical Bulletin, Journal Year: 2024, Volume and Issue: 31(2), P. 95 - 106

Published: April 28, 2024

Background . Autophagy refers to an evolutionary physiological mechanism of cell self-purification. The use its positive effects in regenerative and preventive medicine implies this process be investigated under the conditions various therapeutic factors. Objective To investigate influence gender, age somatic-morphological factors on autophagy processes induced by a comprehensive wellness program as expressed marker protein Beclin-1. Methods A prospective observational study involved 107 patients monitored before after procedures. All participants underwent clinic OOO Centr Zdorovie (Maykop, Russia). was conducted periods September‒December 2020 January 2021. measuring concentration Beclin-1 blood 12 days In order characterize degree activation process, index “delta-Beclin-1” introduced (difference between initial level registered days). response evaluated terms upward or downward direction (positive/negative delta- value, respectively). effect procedures functional systems organism determined accordance with laboratory tests (complete count, lipid profile), morphometric evaluation, assessment body composition impedance monitoring. obtained data were processed using SPSS Statistics 26.0 (IBM, USA). Results Monitoring revealed that basic activity is age-specific significantly lower individuals over 60 years compared both healthy persons. This correlation appears more pronounced obese (r = -0.59). men 30% than women, however, set mentioned increased 7%. found not all responded unidirectionally procedures: 49 out 77 showed increase concentration, while 28 — decrease. Patients who participated demonstrated negative expression age, those responding mass index, weight fat mass. source can refer mass, women lean especially mature period II (36‒60 years). Conclusion complex include gender weight. These obtain different significance at periods. serve alternative existing pharmacological methods for activating humans.

Language: Английский

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Harnessing immunomodulation to combat sarcopenia: current insights and possible approaches

Immunity & Ageing, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 5, 2024

Sarcopenia is a complex age-associated syndrome of progressive loss muscle mass and strength. Although this condition influenced by many factors, age-related changes in immune function including cell dynamics, chronic inflammation contribute to its progression. The interplay between the system, gut-muscle axis, autophagy further underscores their important roles sarcopenia pathogenesis. Immunomodulation has emerged as promising strategy counteract sarcopenia. Traditional management approaches treat physical exercise nutritional supplementation, emerging technologies biophysical stimulation demonstrated importance immunomodulation regulation macrophages T cells reduction inflammation. Treatments alleviate low-grade older adults modulating gut microbial composition diversity combat Furthermore, some pharmacological interventions, nano-medicine, therapies targeting muscle, microbiota, or present additional avenues for This narrative review explores immunological underpinnings sarcopenia, elucidating relationship system during ageing. Additionally, discusses new areas such axis autophagy, which bridge health. Insights into current potential through modulation are provided, along with suggestions future research directions therapeutic strategies. We aim guide investigation clinical biomarkers identify indicators diagnosis treatment targets condition. also draw attention considering

Language: Английский

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Irisin in degenerative musculoskeletal diseases: functions in system and potential in therapy

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107480 - 107480

Published: Oct. 25, 2024

Language: Английский

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Self-Reported Sleep Duration Is a Useful Tool to Predict Sarcopenia in Chilean Older Adults: Evidence from the ALEXANDROS Longitudinal Study

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(6), P. 578 - 578

Published: May 28, 2024

Age-related sleep disorders share common pathways with sarcopenia. Prospective data from Latin American populations are scarce, and the association between sarcopenia in Chileans remains unknown. Thus, we aimed to study longitudinal a cohort of 1116 community-dwelling Chilean older people ≥60 years old ALEXANDROS cohorts. After exclusion criteria, 318 subjects were followed. Sociodemographic data, self-reported chronic diseases, sedentarism, characteristics, anthropometric measurements, handgrip strength, muscle performance assessed. Results indicated that at baseline, prevalence was 24.10% without gender differences, problems 23.3%, higher women (26.46% versus 17.15% men). The adjusted Cox regression models for showed an sarcopenia, (HR = 2.08, 95% IC 1.14–3.80), long duration 2.42, 1.20–4.91). 8.24 follow-up, there 2.2 cases per 100 person-years. This demonstrates independent risk factor people. identification through provides opportunity early cost-effective prevention.

Language: Английский

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Deciphering Osteosarcopenia through the hallmarks of aging

Mechanisms of Ageing and Development, Journal Year: 2024, Volume and Issue: 222, P. 111997 - 111997

Published: Oct. 11, 2024

Osteosarcopenia is a major driver of functional loss and risk factor for falls, fractures, disability mortality in older adults, urgently requiring the development effective interventions to address it. The hallmarks aging provide theoretical practical framework that allows structured organization current knowledge planning new lines. This article comprehensively reviews currently available literature on role osteosarcopenia, thereby offering panoramic view state art gaps this field.

Language: Английский

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