International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12672 - 12672
Опубликована: Ноя. 26, 2024
Neurodegenerative
diseases
are
characterized
by
brain
lesions
that
limit
normal
daily
activities
and
represent
a
major
challenge
to
healthcare
systems
worldwide,
with
significant
economic
impact.
Nanotechnology
is
the
science
of
manipulating
matter
at
nanoscale,
where
materials
exhibit
unique
properties
significantly
different
from
their
larger
counterparts.
These
can
be
exploited
for
wide
range
applications,
including
medicine.
Among
emerging
therapeutic
approaches
treatment
neurodegenerative
diseases,
nanotechnologies
gaining
prominence
as
promising
avenue
explore.
Here,
we
review
state
art
biological
artificial
vesicles
in
context
diseases.
Indeed,
nanometric
structures
such
extracellular
tool
disorders
due
size,
biocompatibility,
ability
transport
drugs,
proteins,
genetic
material
across
blood-brain
barrier
target
specific
cells
areas.
In
future,
deeper
broader
synergy
between
science,
bioengineering,
biology,
medicine,
discovery
new,
increasingly
powerful
delivery
will
certainly
enable
more
applied
use
nanotechnology
disorders.
Pharmacological Research,
Год журнала:
2024,
Номер
204, С. 107202 - 107202
Опубликована: Май 3, 2024
Plant-derived
extracellular
vesicles
(PDEV)
constitute
nanoscale
entities
comprising
lipids,
proteins,
nucleic
acids
and
various
components
enveloped
by
the
lipid
bilayers
of
plant
cells.
These
play
a
crucial
role
in
facilitating
substance
information
transfer
not
only
between
cells
but
also
across
different
species.
Owing
to
its
safety,
stability,
abundance
raw
materials,
this
has
found
extensive
utilization
recent
years
within
research
endeavors
aimed
at
treating
diseases.
This
article
provides
an
overview
pathways
biological
characteristics
PDEV,
along
with
prevalent
methods
employed
for
isolation,
purification,
storage.
Furthermore,
we
comprehensively
outline
therapeutic
implications
diverse
sources
PDEV
musculoskeletal
system
disorders.
Additionally,
explore
as
platforms
engineering
drug
carriers,
aiming
delve
deeper
into
significance
potential
contributions
realm
system.
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
170, С. 116098 - 116098
Опубликована: Дек. 28, 2023
Inflammatory
bowel
diseases
(IBD),
such
as
severe
colitis,
are
associated
with
the
development
of
lung
inflammation
and
tissue
damage.
Pueraria
lobata
(P.
lobata)
plays
an
essential
role
in
controlling
cytokines.
However,
exact
mechanism
response
is
still
unknown.
Purpose:
To
investigate
effects
P.
lobata-derived
exosomes-like
nanovesicles
(PLDENs)
on
colitis
their
inflammatory
response.
In
this
study,
we
investigated
PLDENs
dextran
sulfate
sodium
(DSS)-induced
explored
mechanisms
by
forming
gut-lung
axis.
were
characterized
mass
spectrometry-based
proteomic
analysis.
The
results
showed
that
had
significant
preventive
DSS-induced
pathological
changes
colons
a
dose-dependent
manner.
Simultaneously,
treatment
could
effectively
reduce
lung.
selectively
regulate
composition
gut
microbiota.
These
data
suggested
'attenuate
inflammation,
providing
efficacious
supplement
for
reducing
co-morbidities
IBD
patients.
Pharmaceutics,
Год журнала:
2024,
Номер
16(4), С. 567 - 567
Опубликована: Апрель 21, 2024
Extracellular
vesicles
(EVs),
acting
as
inherent
nanocarriers
adept
at
transporting
a
range
of
different
biological
molecules
such
proteins,
lipids,
and
genetic
material,
exhibit
diverse
functions
within
the
gastroenteric
tract.
In
states
normal
health,
they
participate
in
upkeep
systemic
organ
homeostasis.
Conversely,
pathological
conditions,
significantly
contribute
to
pathogenesis
gastrointestinal
diseases
(GIDs).
Isolating
EVs
from
patients'
biofluids
facilitates
discovery
new
biomarkers
that
have
potential
offer
rapid,
cost-effective,
non-invasive
method
for
diagnosing
prognosing
specific
GIDs.
Furthermore,
demonstrate
considerable
therapeutic
naturally
targeted
physiological
carriers
intercellular
delivery
cargo
or
nanoscale
tools
engineered
specifically
regulate
physio-pathological
conditions
disease
progression.
Their
attributes
including
safety,
high
permeability,
stability,
biocompatibility,
low
immunogenicity,
homing/tropism
capabilities
their
promising
clinical
applications.
This
review
will
delve
into
various
examples
serving
context
GIDs,
highlighting
both
functional
structural
conditions.
The
versatile
advantageous
properties
position
them
candidates
innovative
strategies
advancing
personalized
medicine
approaches
tailored
tract,
addressing
Nutrients,
Год журнала:
2024,
Номер
16(18), С. 3097 - 3097
Опубликована: Сен. 13, 2024
Background/Objectives:
Extracellular
vesicles
(EVs)
play
a
key
role
in
intercellular
communication
by
transferring
miRNAs
and
other
macromolecules
between
cells.
Understanding
how
diet
exercise
modulate
the
release
content
of
skeletal
muscle
(SM)-derived
EVs
could
lead
to
novel
therapeutic
strategies
prevent
age-related
decline
chronic
diseases,
such
as
sarcopenia.
This
review
aims
provide
an
overview
function
explore
nutritional
physical
interventions
can
optimise
their
function.
Methods:
A
literature
studies
examining
impact
on
MS-derived
was
conducted.
Major
scientific
databases,
including
PubMed,
Scopus
Web
Science,
were
searched
using
keywords
‘extracellular
vesicles’,
‘muscle’,
‘exercise’,
‘nutrition’
‘sarcopenia’.
The
selected
included
randomised
controlled
trials
(RCTs),
clinical
cohort
studies.
Data
from
these
synthesised
identify
findings
related
EVs,
composition
potential
targets.
Results:
Dietary
patterns,
specific
foods
supplements
found
significantly
EV
composition,
affecting
health
metabolism.
Exercise-induced
changes
observed
after
both
acute
interventions,
with
marked
proteins
growth
inflammation.
Nutritional
Mediterranean
omega-3
fatty
acids,
have
also
shown
ability
alter
profiles,
suggesting
improve
cardiovascular
reduce
Conclusions:
are
emerging
critical
mediators
beneficial
effects
health.
Both
offering
promising
avenues
for
development
targeting
sarcopenia
diseases.
Future
research
should
focus
large-scale
RCT
standardised
methodologies
better
understand
biomarkers
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 23, 2024
Plant-driven
extracellular
vesicles
(PEVs)
have
attracted
significant
interest
due
to
their
natural
origin,
remarkable
bioactivity,
and
efficacy
in
drug
encapsulation
target
delivery.
In
our
work,
from
Citri
Reticulate
Pericranium
(CEVs)
were
isolated
investigated
physicochemical
characteristics
biological
activities.
We
identified
the
vesicle
structures
as
regular,
with
a
particle
size
of
approximately
200
nm.
also
detected
large
quantities
lipids,
proteins,
carbohydrates,
well
total
phenols
flavonoids.
The
rich
content
CEVs
endows
them
antioxidant
anti-inflammatory
effects,
which
can
notably
increase
levels
GSH
effectively
promote
activity
markers
such
SOD,
CAT,
GR.
Additionally,
they
inhibit
level
inflammatory
like
NO
factors
(
IL-6
,
IL-1β
TNF-α
).
addition,
we
successfully
synthesized
EVs-nob
nanoparticles
83.75%
±
2.83%
rate
2.79%
0.02%
loading,
may
enhance
effects
nobiletin.
Our
research
provides
critical
insights
into
bioactivity
demonstrates
potential
PEVs
nanocarrier
creation,
thereby
promoting
advancement
more
for
biomedical
applications.
International Immunopharmacology,
Год журнала:
2024,
Номер
133, С. 112140 - 112140
Опубликована: Апрель 25, 2024
Inflammation-induced
intestinal
barrier
dysfunction
is
not
only
a
pathological
feature
of
Crohn's
disease
(CD)
but
also
an
important
therapeutic
target.
Sclareol
(SCL)
nontoxic
natural
plant
compound
with
anti-inflammatory
effect,
its
role
in
CD
has
been
established.
In
vivo
studies
mice
TNBS-induced
colitis
were
carried
out
to
evaluate
the
effects
SCL
on
CD-like
and
function.
vitro,
TNF-α-induced
colonic
organoid
model
was
established
test
direct
effect
inflammation-induced
injure
inflammatory
response.
The
Nrf2/NF-κB/MLCK
signalling
analysed
explore
mechanism
SCL.
vivo,
largely
alleviated
TNBS
mice,
as
evidenced
by
improvements
weight
loss,
symptoms,
endoscopic
score,
macroscopic
histological
inflammation
score.
Moreover,
significantly
improved
dysfunction,
manifested
reduced
permeability
decreased
bacterial
translocation
mice.
Importantly,
antagonised
mucosal
while
protecting
tight
junctions
depressed
pro-inflammatory
cytokines
levels
epithelial
model.
context
CD,
protective
against
damage
are
at
least
partially
results
from
Nrf2
activation
NF-κB/MLCK
inhibition.
colitis,
possibly
through
modulation
signalling.
view
SCL's
safety
profile,
there
hope
that
it
will
be
useful
clinic.
Plant-derived
extracellular
vesicles
(EVs)
are
promising
therapeutic
agents
owing
to
their
natural
abundance,
accessibility,
and
unique
biological
properties.
This
review
provides
a
comprehensive
exploration
of
the
potential
plant-derived
EVs
emphasizes
anti-inflammatory,
antimicrobial,
tumor-inhibitory
effects.
Here,
we
discussed
advancements
in
isolation
purification
techniques,
such
as
ultracentrifugation
size-exclusion
chromatography,
which
critical
for
maintaining
functional
integrity
these
nanovesicles.
Next,
investigated
diverse
administration
routes
carefully
weighed
respective
advantages
challenges
related
bioavailability
patient
compliance.
Moreover,
elucidated
multifaceted
mechanisms
action
EVs,
including
roles
anti-inflammation,
antioxidation,
antitumor
activity,
modulation
gut
microbiota.
We
also
impact
on
specific
diseases
cancer
inflammatory
bowel
disease,
highlighting
importance
addressing
current
production
scalability,
regulatory
compliance,
immunogenicity.
Finally,
proposed
future
research
directions
optimizing
EV
extraction
developing
targeted
delivery
systems.
Through
efforts,
envision
seamless
integration
into
mainstream
medicine,
offering
safe
potent
alternatives
across
various
medical
disciplines.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 12793 - 12815
Опубликована: Ноя. 1, 2024
Plant-derived
exosome-like
nanovesicles
(PELNs)
from
edible
plants,
isolated
by
ultracentrifugation,
size
exclusion
chromatography
or
other
methods,
were
proved
to
contain
a
variety
of
biologically
active
and
therapeutically
specific
components.
Recently,
investigations
in
the
field
PELN-based
biomedicine
have
been
conducted,
which
positioned
those
as
promising
tools
for
prevention
treatment
several
diseases,
with
their
natural
origin
potentially
offering
superior
biocompatibility
bioavailability.
However,
inadequate
targeting
limited
therapeutic
effects
constrain
utility
clinical
translation
PELNs.
Thus,
strategies
aiming
at
bridging
gap
engineering
PELNs
great
interest.
Those
approaches
include
membrane
hybridization,
physical
chemical
surface
functionalization
encapsulation
payloads.
Herein,
we
provide
comprehensive
overview
biogenesis
composition,
isolation
purification
methods
characterization
PELNs,
well
functions.
Current
knowledge
on
construction
biomedical
application
engineered
reviewed.
Additionally,
future
directions
perspectives
this
discussed
order
further
enrich
expand
prospects
