Biotechnology for Biofuels and Bioproducts,
Год журнала:
2023,
Номер
16(1)
Опубликована: Июнь 15, 2023
Lignocellulose-derived
aldehyde
inhibitors
seriously
blocked
the
biorefinery
of
biofuels
and
biochemicals.
To
date,
economic
production
lignocellulose-based
products
heavily
relied
on
high
productivities
fermenting
strains.
However,
it
was
expensive
time-consuming
for
achievable
rational
modification
to
strengthen
stress
tolerance
robustness
inhibitors.
Here,
aimed
improve
cellulosic
bioethanol
fermentability
chassis
Zymomonas
mobilis
ZM4
pretreated
using
energy-efficient
eco-friendly
cold
plasma.It
found
that
weaker
in
CSH
(corn
stover
hydrolysates)
than
synthetic
medium
Z.
mobilis,
thus
attributed
inhibition
lignocellulose-derived
CSH.
Convincingly,
further
confirmed
mixed
aldehydes
severely
decreased
accumulation
through
additional
supplementary
assays
medium.
After
assayed
under
different
processing
time
(10-30
s),
discharge
power
(80-160
W),
working
pressure
(120-180
Pa)
atmosphere
plasma
(CAP),
achieved
increased
after
at
optimized
parameters
(20
s,
140
W
165
Pa).
It
showed
brought
about
three
mutation
sites
including
ZMO0694
(E220V),
ZMO0843
(L471L)
(P505H)
via
Genome
resequencing-based
SNPs
(single
nucleotide
polymorphisms).
A
serial
differentially
expressed
genes
(DEGs)
were
identified
as
potential
contributors
RNA-Seq
sequencing,
ZMO0253
ZMO_RS09265
(type
I
secretion
outer
membrane
protein),
ZMO1941
(Type
IV
secretory
pathway
protease
TraF-like
ZMOr003
ZMOr006
(16S
ribosomal
RNA),
ZMO0375
ZMO0374
(levansucrase)
ZMO1705
(thioredoxins).
enriched
cellular
process,
followed
by
metabolic
process
single-organism
biological
process.
For
KEGG
analysis,
mutant
also
referred
starch
sucrose
metabolism,
galactose
metabolism
two-component
system.
Finally,
but
interestingly,
simultaneously
enhanced
capacity
mobilis.Of
several
candidate
genetic
changes,
treated
with
conferred
upon
facilitated
production.
This
work
would
provide
a
strain
biocatalyst
efficient
lignocellulosic
Synthetic and Systems Biotechnology,
Год журнала:
2024,
Номер
9(2), С. 242 - 249
Опубликована: Фев. 13, 2024
Lactate
is
an
important
monomer
for
the
synthesis
of
poly-lactate
(PLA),
which
a
substitute
petrochemical
plastics.
To
achieve
goal
high
lactate
titer,
rate,
and
yield
commercial
production,
efficient
production
pathway
needed
as
well
genetic
targets
that
affect
tolerance.
In
this
study,
LldR-based
d-lactate
biosensor
with
broad
dynamic
range
was
first
applied
into
Zymomonas
mobilis
to
select
mutant
strains
strong
GFP
fluorescence,
could
be
increased
production.
Then,
combined
genome-wide
CRISPR
interference
(CRISPRi)
library
targeting
entire
genome
generate
thousands
mutants
gRNA
different
across
whole
genome.
Specifically,
two
libraries
were
selected
containing
105
104
sites
from
rounds
fluorescence-activated
cell
sorting
(FACS),
respectively.
Two
ZMO1323
ZMO1530
characterized
confirmed
associated
further
knockout
resulted
in
15%
21%
increase
This
work
thus
not
only
established
high-throughput
approach
combines
genome-scale
CRISPRi
biosensor-assisted
screening
identify
Z.
mobilis,
but
also
provided
feasible
rapid
identification
strain
performance
other
industrial
microorganisms.
Bioresource Technology,
Год журнала:
2024,
Номер
399, С. 130594 - 130594
Опубликована: Март 15, 2024
Microbial
oils
are
a
sustainable
biomass-derived
substitute
for
liquid
fuels
and
vegetable
oils.
Oilcane,
an
engineered
sugarcane
with
superior
feedstock
characteristics
biodiesel
production,
is
promising
candidate
bioconversion.
This
study
describes
the
processing
of
oilcane
stems
into
juice
hydrothermally
pretreated
lignocellulosic
hydrolysate
their
valorization
to
ethanol
microbial
oil
using
Saccharomyces
cerevisiae
Rhodosporidium
toruloides
strains,
respectively.
A
bioethanol
titer
106
g/L
was
obtained
from
S.
grown
on
in
3
L
fermenter,
lipid
8.8
R.
75
fermenter.
Oil
extracted
cells
supercritical
CO2,
observed
fatty
acid
profile
consistent
previous
studies
this
strain.
These
results
demonstrate
feasibility
pilot-scale
production
as
part
integrated
bioconversion
strategy.
Microbial
cell
factories
(MCFs)
are
extensively
used
to
produce
a
wide
array
of
bioproducts,
such
as
bioenergy,
biochemical,
food,
nutrients,
and
pharmaceuticals,
have
been
regarded
the
"chips"
biomanufacturing
that
will
fuel
emerging
bioeconomy
era.
Biotechnology
advances
led
screening,
investigation,
engineering
an
increasing
number
microorganisms
diverse
MCFs,
which
workhorses
help
develop
bioeconomy.
This
review
briefly
summarizes
progress
strategies
in
development
robust
efficient
MCFs
for
sustainable
economic
biomanufacturing.
First,
comprehensive
understanding
microbial
chassis
cells,
including
accurate
genome
sequences
corresponding
annotations;
metabolic
regulatory
networks
governing
substances,
energy,
physiology,
information;
their
similarity
uniqueness
compared
with
those
other
microorganisms,
is
needed.
Moreover,
application
effective
tools
crucial
both
model
nonmodel
cells
into
identification
characterization
biological
parts,
well
design,
synthesis,
assembly,
editing,
regulation
genes,
circuits,
pathways.
also
highlights
necessity
integrating
automation
artificial
intelligence
(AI)
biotechnology
facilitate
future
customized
synthetic
expedite
industrialization
process
Synthetic and Systems Biotechnology,
Год журнала:
2024,
Номер
9(2), С. 349 - 358
Опубликована: Март 18, 2024
Due
to
the
complicated
metabolic
and
regulatory
networks
of
l-serine
biosynthesis
degradation,
microbial
cell
factories
for
production
using
non-model
microorganisms
have
not
been
reported.
In
this
study,
a
combination
synthetic
biology
process
optimization
were
applied
in
an
ethanologenic
bacterium
Zymomonas
mobilis
production.
By
blocking
degradation
pathway
while
introducing
exporter
EceamA
from
E.
coli,
titer
recombinant
Z.
was
increased
15.30
mg/L
62.67
mg/L.
It
further
260.33
after
enhancing
pathway.
Then,
536.70
achieved
by
removing
feedback
inhibition
with
SerA
mutant,
elevated
687.67
obtained
through
increasing
serB
copies
precursors.
Finally,
855.66
can
be
accumulated
supplementation
glutamate
precursor.
This
work
thus
only
constructed
producer
help
understand
bottlenecks
limiting
improvement,
but
also
provides
guidance
on
engineering
microbes
produce
biochemicals
pathways
such
as
amino
acids
or
terpenoids.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Апрель 17, 2024
Abstract
Cellulose
is
an
important
abundant
renewable
resource
on
Earth,
and
the
microbial
cellulose
utilization
mechanism
has
attracted
extensive
attention.
Recently,
some
signalling
molecules
have
been
found
to
regulate
discovery
of
underlying
signals
recently
In
this
paper,
we
that
hydrogen
sulfide
(H
2
S)
concentration
under
culture
condition
increased
approximately
2.3-fold
compared
with
glucose
in
Ganoderma
lucidum
.
Further
evidence
shown
cellulase
activities
G.
were
improved
by
18.2-27.6%
through
increasing
H
S
concentration.
Then,
observed
carbon
repressor
CreA
inhibited
biosynthesis
binding
promoter
cbs
,
a
key
gene
for
biosynthesis,
at
“CTGGGG”.
our
study,
reported
first
time
analyzed
induced
cellulose.
This
study
not
only
enriches
understanding
but
also
provides
reference
analysis
physiological
function
signals.
