Cold plasma pretreatment reinforces the lignocellulose-derived aldehyde inhibitors tolerance and bioethanol fermentability for Zymomonas mobilis DOI Creative Commons
Xia Yi, Dong Yang, Xiaoyan Xu

et al.

Biotechnology for Biofuels and Bioproducts, Journal Year: 2023, Volume and Issue: 16(1)

Published: June 15, 2023

Lignocellulose-derived aldehyde inhibitors seriously blocked the biorefinery of biofuels and biochemicals. To date, economic production lignocellulose-based products heavily relied on high productivities fermenting strains. However, it was expensive time-consuming for achievable rational modification to strengthen stress tolerance robustness inhibitors. Here, aimed improve cellulosic bioethanol fermentability chassis Zymomonas mobilis ZM4 pretreated using energy-efficient eco-friendly cold plasma.It found that weaker in CSH (corn stover hydrolysates) than synthetic medium Z. mobilis, thus attributed inhibition lignocellulose-derived CSH. Convincingly, further confirmed mixed aldehydes severely decreased accumulation through additional supplementary assays medium. After assayed under different processing time (10-30 s), discharge power (80-160 W), working pressure (120-180 Pa) atmosphere plasma (CAP), achieved increased after at optimized parameters (20 s, 140 W 165 Pa). It showed brought about three mutation sites including ZMO0694 (E220V), ZMO0843 (L471L) (P505H) via Genome resequencing-based SNPs (single nucleotide polymorphisms). A serial differentially expressed genes (DEGs) were identified as potential contributors RNA-Seq sequencing, ZMO0253 ZMO_RS09265 (type I secretion outer membrane protein), ZMO1941 (Type IV secretory pathway protease TraF-like ZMOr003 ZMOr006 (16S ribosomal RNA), ZMO0375 ZMO0374 (levansucrase) ZMO1705 (thioredoxins). enriched cellular process, followed by metabolic process single-organism biological process. For KEGG analysis, mutant also referred starch sucrose metabolism, galactose metabolism two-component system. Finally, but interestingly, simultaneously enhanced capacity mobilis.Of several candidate genetic changes, treated with conferred upon facilitated production. This work would provide a strain biocatalyst efficient lignocellulosic

Language: Английский

Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production DOI Creative Commons

Qiqun Peng,

Weiwei Bao,

Binan Geng

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(2), P. 242 - 249

Published: Feb. 13, 2024

Lactate is an important monomer for the synthesis of poly-lactate (PLA), which a substitute petrochemical plastics. To achieve goal high lactate titer, rate, and yield commercial production, efficient production pathway needed as well genetic targets that affect tolerance. In this study, LldR-based d-lactate biosensor with broad dynamic range was first applied into Zymomonas mobilis to select mutant strains strong GFP fluorescence, could be increased production. Then, combined genome-wide CRISPR interference (CRISPRi) library targeting entire genome generate thousands mutants gRNA different across whole genome. Specifically, two libraries were selected containing 105 104 sites from rounds fluorescence-activated cell sorting (FACS), respectively. Two ZMO1323 ZMO1530 characterized confirmed associated further knockout resulted in 15% 21% increase This work thus not only established high-throughput approach combines genome-scale CRISPRi biosensor-assisted screening identify Z. mobilis, but also provided feasible rapid identification strain performance other industrial microorganisms.

Language: Английский

Citations

8
Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis DOI
Geng Binan, Yalun Wu, Wu Xinyan

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(11), P. 1551 - 1575

Published: Aug. 28, 2024

Language: Английский

Citations

8
Scale-up of microbial lipid and bioethanol production from oilcane DOI Creative Commons
Narendra Naik Deshavath, William Woodruff, Fred J. Eller

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 399, P. 130594 - 130594

Published: March 15, 2024

Microbial oils are a sustainable biomass-derived substitute for liquid fuels and vegetable oils. Oilcane, an engineered sugarcane with superior feedstock characteristics biodiesel production, is promising candidate bioconversion. This study describes the processing of oilcane stems into juice hydrothermally pretreated lignocellulosic hydrolysate their valorization to ethanol microbial oil using Saccharomyces cerevisiae Rhodosporidium toruloides strains, respectively. A bioethanol titer 106 g/L was obtained from S. grown on in 3 L fermenter, lipid 8.8 R. 75 fermenter. Oil extracted cells supercritical CO2, observed fatty acid profile consistent previous studies this strain. These results demonstrate feasibility pilot-scale production as part integrated bioconversion strategy.

Language: Английский

Citations

7
Microbial cell factories for bioeconomy: From discovery to creation DOI Creative Commons
Xiongying Yan,

Qiaoning He,

Binan Geng

et al.

BioDesign Research, Journal Year: 2024, Volume and Issue: 6

Published: Jan. 1, 2024

Microbial cell factories (MCFs) are extensively used to produce a wide array of bioproducts, such as bioenergy, biochemical, food, nutrients, and pharmaceuticals, have been regarded the "chips" biomanufacturing that will fuel emerging bioeconomy era. Biotechnology advances led screening, investigation, engineering an increasing number microorganisms diverse MCFs, which workhorses help develop bioeconomy. This review briefly summarizes progress strategies in development robust efficient MCFs for sustainable economic biomanufacturing. First, comprehensive understanding microbial chassis cells, including accurate genome sequences corresponding annotations; metabolic regulatory networks governing substances, energy, physiology, information; their similarity uniqueness compared with those other microorganisms, is needed. Moreover, application effective tools crucial both model nonmodel cells into identification characterization biological parts, well design, synthesis, assembly, editing, regulation genes, circuits, pathways. also highlights necessity integrating automation artificial intelligence (AI) biotechnology facilitate future customized synthetic expedite industrialization process

Language: Английский

Citations

4
Adaptive laboratory evolution boosts Yarrowia lipolytica tolerance to vanillic acid DOI

Yuanyuan Sha,

Linlin Zhou,

Zedi Wang

et al.

Journal of Biotechnology, Journal Year: 2023, Volume and Issue: 367, P. 42 - 52

Published: March 23, 2023

Language: Английский

Citations

10
The CBS/H2S signalling pathway regulated by the carbon repressor CreA promotes cellulose utilization in Ganoderma lucidum DOI Creative Commons

Jiaolei Shangguan,

Jinjin Qiao,

He Liu

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 17, 2024

Abstract Cellulose is an important abundant renewable resource on Earth, and the microbial cellulose utilization mechanism has attracted extensive attention. Recently, some signalling molecules have been found to regulate discovery of underlying signals recently In this paper, we that hydrogen sulfide (H 2 S) concentration under culture condition increased approximately 2.3-fold compared with glucose in Ganoderma lucidum . Further evidence shown cellulase activities G. were improved by 18.2-27.6% through increasing H S concentration. Then, observed carbon repressor CreA inhibited biosynthesis binding promoter cbs , a key gene for biosynthesis, at “CTGGGG”. our study, reported first time analyzed induced cellulose. This study not only enriches understanding but also provides reference analysis physiological function signals.

Language: Английский

Citations

3
Effect of acetate and format inhibitors on the production of Aspergillus niger inulinase and fermentation modeling, sensitivity, and economic analysis DOI Creative Commons

Hilal Nur Gürler Tufan,

Selime Benemir Erkan Ünsal, Müge Canatar

et al.

Biomass Conversion and Biorefinery, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Language: Английский

Citations

0
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production DOI Creative Commons

Jiyun Lou,

Xia Wang, Runxia Li

et al.

Synthetic and Systems Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Robust Saccharomyces cerevisiae by rational metabolic engineering for effective ethanol production from undetoxified steam-exploded corn stover hydrolysate DOI
Yilu Wu, Di Cai,

Changsheng Su

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132605 - 132605

Published: April 1, 2025

Language: Английский

Citations

0
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production DOI Creative Commons
Zhen Wang, Xia Wang, Xiongying Yan

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(2), P. 349 - 358

Published: March 18, 2024

Due to the complicated metabolic and regulatory networks of l-serine biosynthesis degradation, microbial cell factories for production using non-model microorganisms have not been reported. In this study, a combination synthetic biology process optimization were applied in an ethanologenic bacterium Zymomonas mobilis production. By blocking degradation pathway while introducing exporter EceamA from E. coli, titer recombinant Z. was increased 15.30 mg/L 62.67 mg/L. It further 260.33 after enhancing pathway. Then, 536.70 achieved by removing feedback inhibition with SerA mutant, elevated 687.67 obtained through increasing serB copies precursors. Finally, 855.66 can be accumulated supplementation glutamate precursor. This work thus only constructed producer help understand bottlenecks limiting improvement, but also provides guidance on engineering microbes produce biochemicals pathways such as amino acids or terpenoids.

Language: Английский

Citations

3