Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 22, 2024

In modern ecological systems, the overuse and misuse of antibiotics have escalated prevalence antibiotic resistance genes (ARGs) mobile genetic elements (MGEs), positioning them as emerging environmental contaminants. Notably, composting serves a sustainable method to recycle agricultural waste into nutrient-rich fertilizer while potentially reducing ARGs MGEs. This study conducted 47-day experiment using pig manure corn straw, supplemented with chitin N-Acetyl-D-glucosamine, explore impact these additives on dynamics MGEs, unravel interplay between microbial communities in composting. Results showed that adding 5% significantly postponed thermophilic phase, yet enhanced removal efficiency total MGEs by over 20% compared control. Additionally, addition N-Acetyl-D-glucosamine increased abundance tetracycline-resistant sulfonamide-resistant genes, well High-throughput sequencing revealed bacterial α-diversity, providing diverse hosts for Resistance mechanisms, predominantly efflux pumps deactivation, played pivotal role shaping resistome process. Co-occurrence network analysis identified key phyla Proteobacteria , Firmicutes Gemmatimonadota Myxococcota transformation dissemination. Redundancy indicated physicochemical factors, particularly carbon-to-nitrogen ratio emerged critical variables influencing The findings lay foundation developing regulation reduce risks animal composts.

Язык: Английский