npj Systems Biology and Applications,
Год журнала:
2023,
Номер
9(1)
Опубликована: Ноя. 30, 2023
Abstract
Recent
global
events
have
drawn
into
focus
the
diversity
of
options
for
combatting
disease
across
a
spectrum
prophylactic
and
therapeutic
approaches.
The
recent
success
mRNA-based
COVID-19
vaccines
has
paved
way
RNA-based
treatments
to
revolutionize
pharmaceutical
industry.
However,
historical
treatment
are
continuously
updated
reimagined
in
context
novel
technical
developments,
such
as
those
facilitated
through
application
synthetic
biology.
When
it
comes
development
genetic
forms
therapies
vaccines,
biology
offers
diverse
tools
approaches
influence
content,
dosage,
breadth
with
prospect
economic
advantage
provided
time
cost
benefits.
This
can
be
achieved
by
utilizing
broad
within
this
discipline
enhance
functionality
efficacy
agent
sequences.
review
will
describe
how
principles
augment
optimizing
not
only
vaccine
antigen,
construct,
activity,
delivery
vector.
enhancement
RNA
technology
implementing
potential
shape
next
generation
therapeutics.
Chemical Communications,
Год журнала:
2022,
Номер
58(54), С. 7562 - 7565
Опубликована: Янв. 1, 2022
This
work
reports
on
the
proof-of-concept
of
a
photoelectrochemical
(PEC)
biosensor
with
horseradish
peroxidase-single
stranded
DNA-encoded
magnetic
bead
(MB-ssDNA-HRP)
signal
probe
cleaved
by
catalytic
hairpin
assembly
(CHA)-mediated
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-Cas12a
system
for
quantification
microRNA
(miR-21)
using
yolk-in-shell
Au@CdS
as
photoactive
material.
Abstract
Gastric
cancer
(GC)
is
one
of
the
most
common
tumors
worldwide
and
leading
cause
tumor-related
mortality.
Endoscopy
serological
tumor
marker
testing
are
currently
main
methods
GC
screening,
treatment
relies
on
surgical
resection
or
chemotherapy.
However,
traditional
examination
more
harmful
to
patients
less
sensitive
accurate.
A
minimally
invasive
method
respond
early
prognosis
monitoring,
efficacy,
drug
resistance
situations
urgently
needed.
As
a
result,
liquid
biopsy
techniques
have
received
much
attention
in
clinical
application
GC.
The
non-invasive
technique
requires
fewer
samples,
reproducible,
can
guide
individualized
patient
by
monitoring
patients'
molecular-level
changes
real-time.
In
this
review,
we
introduced
applications
circulating
cells,
free
DNA,
non-coding
RNAs,
exosomes,
proteins,
which
primary
markers
technology
We
also
discuss
current
limitations
future
trends
as
applied
technology.
Heliyon,
Год журнала:
2024,
Номер
10(4), С. e26179 - e26179
Опубликована: Фев. 1, 2024
CRISPR/Cas
systems
have
become
integral
parts
of
nucleic
acid
detection
apparatus
and
biosensors.
Various
such
as
CRISPR/Cas9,
CRISPR/Cas12,
CRISPR/Cas13,
CRISPR/Cas14
CRISPR/Cas3
utilize
different
mechanisms
to
detect
or
differentiate
biological
activities
nucleotide
sequences.
Usually,
CRISPR/Cas-based
are
combined
with
polymerase
chain
reaction,
loop-mediated
isothermal
amplification,
recombinase
amplification
transcriptional
technologies
for
effective
diagnostics.
Premised
on
these,
many
biosensors
been
developed
acids
viral
bacterial
pathogens
in
clinical
samples,
well
other
applications
life
sciences
including
biosecurity,
food
safety
environmental
assessment.
Additionally,
showed
better
specificity
compared
molecular
diagnostic
methods.
In
this
review,
we
give
an
overview
various
methods
highlight
some
advances
their
development
components.
We
also
discourse
operational
challenges
advantages
disadvantages
systems.
Finally,
important
considerations
offered
the
improvement
testing.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(24), С. 28933 - 28940
Опубликована: Июнь 9, 2023
MicroRNA
(miRNA)
is
a
promising
biomarker
for
the
diagnosis,
monitoring,
and
prognostic
evaluation
of
diseases,
especially
cancer.
The
existing
miRNA
detection
methods
usually
need
external
instruments
quantitative
signal
output,
limiting
their
practical
applications
in
point-of-care
(POC)
settings.
Here,
we
propose
distance-based
biosensor
through
responsive
hydrogel,
combination
with
CRISPR/Cas12a
system
target-triggered
strand
displacement
amplification
(SDA)
reaction
visual
sensitive
measurement
miRNA.
target
first
converted
into
plenty
double-stranded
DNA
(dsDNA)
via
SDA
reaction.
Then,
dsDNA
products
trigger
collateral
cleavage
activity
CRISPR/Cas12a,
leading
to
release
trypsin
from
magnetic
beads
(MBs).
released
can
hydrolyze
gelatin,
hence
permeability
gelatin-treated
filter
paper
increased,
resulting
visible
distance
on
cotton
thread.
Using
this
system,
concentration
be
quantified
visually
without
any
assistance
instruments,
limit
6.28
pM
obtained.
In
addition,
human
serum
samples
cell
lysates
also
detected
accurately.
Owing
characteristics
simplicity,
sensitivity,
specificity,
portability,
proposed
provides
new
tool
holds
great
promise
POC
applications.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(2), С. 910 - 916
Опубликована: Янв. 3, 2024
Early
tumor
diagnosis
is
crucial
to
successful
treatment.
Earlier
studies
have
shown
that
microRNA
a
biomarker
for
early
diagnosis.
The
development
of
highly
sensitive
miRNA
detection
methods,
especially
in
living
cells,
plays
an
indispensable
role
and
treatment
tumor.
Although
the
catalytic
hairpin
assembly
(CHA)-based
analysis
strategy
commonly
used
disease
diagnosis,
further
application
CHA
hindered
due
its
low
amplification
efficiency
recognition
contrast.
To
address
these
limitations,
we
propose
dual-signal
based
on
APE1-assisted
amplification,
enabling
high-contrast
imaging.
miR-221
was
selected
as
target
model.
This
has
exhibited
high
efficiency,
which
could
analyze
21
fM.
also
specificity,
distinguish
nontarget
with
single-base
differences.
Moreover,
this
method
showed
significant
potential
practical
application,
it
successfully
expression
difference
plasma
samples
normal
people
patients.
Most
importantly,
level
APE1
enzyme
cells
higher
than
allowing
sensitively
specifically
image
within
cells.
proposed
been
indicate
fluctuations
intracellular
between
cancer
We
anticipate
will
provide
fresh
insights
can
be
powerful
tool
analysis.
