Photoelectrochemical bioanalysis of microRNA on yolk-in-shell Au@CdS based on the catalytic hairpin assembly-mediated CRISPR-Cas12a system

Chemical Communications, Journal Year: 2022, Volume and Issue: 58(54), P. 7562 - 7565

Published: Jan. 1, 2022

This work reports on the proof-of-concept of a photoelectrochemical (PEC) biosensor with horseradish peroxidase-single stranded DNA-encoded magnetic bead (MB-ssDNA-HRP) signal probe cleaved by catalytic hairpin assembly (CHA)-mediated clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system for quantification microRNA (miR-21) using yolk-in-shell Au@CdS as photoactive material.

Language: Английский

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Clinical application and detection techniques of liquid biopsy in gastric cancer

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Jan. 11, 2023

Abstract Gastric cancer (GC) is one of the most common tumors worldwide and leading cause tumor-related mortality. Endoscopy serological tumor marker testing are currently main methods GC screening, treatment relies on surgical resection or chemotherapy. However, traditional examination more harmful to patients less sensitive accurate. A minimally invasive method respond early prognosis monitoring, efficacy, drug resistance situations urgently needed. As a result, liquid biopsy techniques have received much attention in clinical application GC. The non-invasive technique requires fewer samples, reproducible, can guide individualized patient by monitoring patients' molecular-level changes real-time. In this review, we introduced applications circulating cells, free DNA, non-coding RNAs, exosomes, proteins, which primary markers technology We also discuss current limitations future trends as applied technology.

Language: Английский

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Nucleic acid-assisted CRISPR-Cas systems for advanced biosensing and bioimaging

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 159, P. 116931 - 116931

Published: Jan. 11, 2023

Language: Английский

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CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges

Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e26179 - e26179

Published: Feb. 1, 2024

CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 CRISPR/Cas3 utilize different mechanisms to detect or differentiate biological activities nucleotide sequences. Usually, CRISPR/Cas-based are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase amplification transcriptional technologies for effective diagnostics. Premised on these, many biosensors been developed acids viral bacterial pathogens in clinical samples, well other applications life sciences including biosecurity, food safety environmental assessment. Additionally, showed better specificity compared molecular diagnostic methods. In this review, we give an overview various methods highlight some advances their development components. We also discourse operational challenges advantages disadvantages systems. Finally, important considerations offered the improvement testing.

Language: Английский

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Dual-Signal Amplification Strategy Based on Catalytic Hairpin Assembly and APE1-Assisted Amplification for High-Contrast miRNA Imaging in Living Cells

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(2), P. 910 - 916

Published: Jan. 3, 2024

Early tumor diagnosis is crucial to successful treatment. Earlier studies have shown that microRNA a biomarker for early diagnosis. The development of highly sensitive miRNA detection methods, especially in living cells, plays an indispensable role and treatment tumor. Although the catalytic hairpin assembly (CHA)-based analysis strategy commonly used disease diagnosis, further application CHA hindered due its low amplification efficiency recognition contrast. To address these limitations, we propose dual-signal based on APE1-assisted amplification, enabling high-contrast imaging. miR-221 was selected as target model. This has exhibited high efficiency, which could analyze 21 fM. also specificity, distinguish nontarget with single-base differences. Moreover, this method showed significant potential practical application, it successfully expression difference plasma samples normal people patients. Most importantly, level APE1 enzyme cells higher than allowing sensitively specifically image within cells. proposed been indicate fluctuations intracellular between cancer We anticipate will provide fresh insights can be powerful tool analysis.

Language: Английский

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Ultrasensitive Detection of miRNA via CRISPR/Cas12a Coupled with Strand Displacement Amplification Reaction

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(24), P. 28933 - 28940

Published: June 9, 2023

MicroRNA (miRNA) is a promising biomarker for the diagnosis, monitoring, and prognostic evaluation of diseases, especially cancer. The existing miRNA detection methods usually need external instruments quantitative signal output, limiting their practical applications in point-of-care (POC) settings. Here, we propose distance-based biosensor through responsive hydrogel, combination with CRISPR/Cas12a system target-triggered strand displacement amplification (SDA) reaction visual sensitive measurement miRNA. target first converted into plenty double-stranded DNA (dsDNA) via SDA reaction. Then, dsDNA products trigger collateral cleavage activity CRISPR/Cas12a, leading to release trypsin from magnetic beads (MBs). released can hydrolyze gelatin, hence permeability gelatin-treated filter paper increased, resulting visible distance on cotton thread. Using this system, concentration be quantified visually without any assistance instruments, limit 6.28 pM obtained. In addition, human serum samples cell lysates also detected accurately. Owing characteristics simplicity, sensitivity, specificity, portability, proposed provides new tool holds great promise POC applications.

Language: Английский

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Amplification-free detection of HBV DNA mediated by CRISPR-Cas12a using surface-enhanced Raman spectroscopy

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1245, P. 340864 - 340864

Published: Jan. 19, 2023

Language: Английский

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Locking-DNA network regulated CRISPR-Cas12a fluorescent aptasensor based on hollow flower-like magnetic MoS2 microspheres for sensitive detection of sulfadimethoxine

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 459, P. 141463 - 141463

Published: Jan. 16, 2023

Language: Английский

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Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1303, P. 342477 - 342477

Published: March 22, 2024

Language: Английский

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An ultrasensitive one-pot Cas13a-based microfluidic assay for rapid multiplexed detection of microRNAs

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117212 - 117212

Published: Jan. 1, 2025

Language: Английский

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