Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108609 - 108609
Опубликована: Май 1, 2025
Язык: Английский
Analytical Chemistry, Год журнала: 2024, Номер 96(18), С. 7274 - 7280
Опубликована: Апрель 24, 2024
Inspired by natural DNA networks, programmable artificial networks have become an attractive tool for developing high-performance biosensors. However, there is still a lot of room expansion in terms sensitivity, atom economy, and result self-validation current microRNA sensors. In this protocol, miRNA-122 as target model, ultrasensitive fluorescence (FL) photoelectrochemical (PEC) dual-mode biosensing platform developed using entropy-driven circuit (EDC) cascaded self-feedback DNAzyme network. The well-designed EDC realizes full utilization the strands improves atomic economy signal amplification system. unique rational design double-CdSe quantum-dot-released substrate network significantly avoids high background signals enhances sensitivity specificity. Also, enzyme-free, effectively risk leakage accuracy sensor. Moreover, introduction superparamagnetic Fe
Язык: Английский
Процитировано
25
Deleted Journal, Год журнала: 2023, Номер 2(1)
Опубликована: Дек. 19, 2023
Abstract Monitoring tumor biomarkers in a non‐invasive manner for diagnosis has attracted increasing attention. Liquid biopsy mainly includes three types of biomarkers: circulating cells, extracellular vesicles, and nucleic acids. These released by cells can travel to other parts the circulation system. The analysis markers enables early diagnosis, progression evolution, treatment monitoring, which are important individualized clinical decisions. In this study, we summarize different DNA nanotechnology strategies that be used capture, amplify, measure discuss current challenges outlook.
Язык: Английский
Процитировано
30
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Фев. 6, 2024
Major health events caused by pathogenic microorganisms are increasing, seriously jeopardizing human lives. Currently PCR and ITA widely used for rapid testing in food, medicine, industry agriculture. However, due to the non-specificity of amplification process, researchers have proposed combination nucleic acid technology with novel CRISPR detection, which improves specificity credibility results. This paper summarizes research progress conjunction CRISPR/Cas detection pathogens, provides a reference theoretical basis subsequent application field pathogen detection.
Язык: Английский
Процитировано
10
ACS Sensors, Год журнала: 2024, Номер 9(3), С. 1438 - 1446
Опубликована: Март 7, 2024
Exosomal microRNAs (exomiRNAs) have emerged as promising biomarkers for the early clinical diagnosis of osteoporosis. However, their limited abundance and short length in peripheral blood present significant challenges accurate detection exomiRNAs. Herein, we designed implemented an efficacious fluorescence-based biosensor highly sensitive exomiRNA associated with osteoporosis, leveraging enhancing 3D DNA walker-induced CRISPR/Cas12a technology. The engineered walker is capable efficiently transforming target into amplifying strands, thereby sensitivity developed biosensor. Concurrently, liberated strands serve activators to trigger Cas12a trans-cleavage activity, culminating a significantly amplified fluorescent signal exomiRNA-214. Under optimal conditions, devised technology demonstrated capacity detect exomiRNA-214 at concentrations low 20.42 fM, encompassing wide linear range extending from 50.0 fM 10.0 nM. Moreover, could accurately differentiate between healthy individuals osteoporosis patients via exomiRNA-214, which was agreement RT-qPCR results. As such, this biosensing offers promise valuable tool
Язык: Английский
Процитировано
10
Molecular Biology Reports, Год журнала: 2025, Номер 52(1)
Опубликована: Янв. 11, 2025
Язык: Английский
Процитировано
1
ACS Omega, Год журнала: 2025, Номер 10(9), С. 8816 - 8831
Опубликована: Март 3, 2025
Early diagnosis of cancer can significantly contribute to improving therapeutic outcomes and enhancing survival rates for patients. Polymer-based biosensors have emerged as a promising tool detection due their high sensitivity, selectivity, low cost. These utilize functionalized polymers in different parts the body detect biomarkers biological samples. This approach offers several advantages over traditional methods, including real-time monitoring noninvasive while maintaining sensitivity accuracy. review discusses recent advances development polymer-based design, fabrication, performance. The essential characteristics biosensing devices are presented, along with examples natural synthetic commonly utilized biosensors. Furthermore, strategies employed tailor improve applications future perspectives application also highlighted. Integrating these advancements will illuminate potential transformative tools early management cancer.
Язык: Английский
Процитировано
1
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 178, С. 117856 - 117856
Опубликована: Июль 9, 2024
Язык: Английский
Процитировано
9
Critical Reviews in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 1 - 17
Опубликована: Март 15, 2024
MicroRNA (miRNA) has emerged as a promising biomarker for disease diagnosis and potential therapeutic targets drug development. The detection of miRNA can serve noninvasive tool in diseases predicting prognosis. CRISPR/Cas12a system great nucleic acid due to its high sensitivity specificity, which been developed be versatile acid-based various fields. However, conversion from RNA DNA with or without amplification operation is necessary based on system, because dsDNA containing PAM sequence ssDNA traditionally considered the activator Cas12a. Until recently, direct by reported. In this review, we provide an overview evolution biosensors indirect direct, would beneficial development CRISPR/Cas12a-based sensors better performance miRNA.
Язык: Английский
Процитировано
8
Biosensors, Год журнала: 2023, Номер 13(9), С. 885 - 885
Опубликована: Сен. 15, 2023
Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify amplification. Recently, numerous attempts been made to incorporate paper microfluidics into these isothermal tests. Paper (including lateral flow strips) used extract acids, amplify the target gene, detect amplified products, all toward automating process. We investigated literature from 2020 present, i.e., since onset of COVID-19 pandemic, during which significant surge in has observed. microfluidic detection extensively for recombinase (RPA) its related methods, along with loop-mediated (LAMP) rolling circle (RCA). Detection was conducted primarily colorimetric fluorometric although few publications demonstrated distance- surface-enhanced Raman spectroscopic (SERS)-based detection. A good number could be found that both on platforms. small showed extraction or three procedures (i.e., fully integrated systems) platforms, necessitating need future work.
Язык: Английский
Процитировано
15
Nano Letters, Год журнала: 2024, Номер unknown
Опубликована: Сен. 17, 2024
MicroRNAs (MiRNAs) are valuable biomarkers for the diagnosis and prognosis of diseases. The development reliable assays is an urgent pursuit. We herein fabricate a novel electrochemical sensing strategy based on conformation transitions DNA nanostructures click chemistry. Duplex-specific nuclease (DSN)-catalyzed reaction first used disintegration triangular pyramid frustum (DNA TPF). A triangle formed, which in turn assists strain-promoted alkyne-azide cycloaddition (SPAAC) to localize single-stranded probes (P1). After SPAAC ligation, multiple hairpins spontaneously folded, labeled species dragged near electrode interface. By recording analyzing responses, highly sensitive biosensor established, exhibits high sensitivity reproducibility. Clinical applications have been verified with good stability. This relies integration chemistry, may inspire further designs nanotechnology clinical
Язык: Английский
Процитировано
6