Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

Analytical Chemistry, Год журнала: 2023, Номер 95(6), С. 3332 - 3339

Опубликована: Янв. 30, 2023

Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) introduced convert each target RNA strand into long single-stranded DNA with repeated sequences, which acted as triggers initiate transcleavage activity of CRISPR-Cas12a. activated Cas12a could cleave biotinylated linker CuS nanoparticles (NPs) inhibit binding NPs streptavidin immobilized surface microplate, strongly reduced generation Cu2+ from between AgNO3, thus efficiently suppressed CL Cu2+-luminol-H2O2 system, giving "signal off" biosensor. With multiple amplification, detection limit developed sensor for miRNA-21 reached 16 aM. In addition, this is not only suitable professional instrument but also smartphone used tool purpose portable low-cost assay. This method be specifically quite low level in human serum samples various cancer cells, indicating its potential ultrasensitive molecular diagnostics.

Язык: Английский

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Recent development of chemiluminescence for bioanalysis

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 166, С. 117213 - 117213

Опубликована: Авг. 4, 2023

Язык: Английский

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Universal smartphone-assisted label-free CRISPR/Cas12a-DNAzyme chemiluminescence biosensing platform for on-site detection of nucleic acid and non-nucleic acid targets

Biosensors and Bioelectronics, Год журнала: 2023, Номер 247, С. 115929 - 115929

Опубликована: Дек. 15, 2023

Язык: Английский

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A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

Biosensors and Bioelectronics, Год журнала: 2023, Номер 225, С. 115102 - 115102

Опубликована: Янв. 25, 2023

Язык: Английский

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Isothermal nucleic acid amplification based microfluidic “lab-on-a-chip” for the detection of pathogenic bacteria and viruses in agri-foods

Trends in Food Science & Technology, Год журнала: 2024, Номер 148, С. 104482 - 104482

Опубликована: Апрель 7, 2024

Язык: Английский

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Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 6, 2024

Major health events caused by pathogenic microorganisms are increasing, seriously jeopardizing human lives. Currently PCR and ITA widely used for rapid testing in food, medicine, industry agriculture. However, due to the non-specificity of amplification process, researchers have proposed combination nucleic acid technology with novel CRISPR detection, which improves specificity credibility results. This paper summarizes research progress conjunction CRISPR/Cas detection pathogens, provides a reference theoretical basis subsequent application field pathogen detection.

Язык: Английский

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A ratiometric electrochemical aptasensor for sensitive detection of kanamycin in food based on entropy-driven strand displacement reaction

Food Control, Год журнала: 2024, Номер 161, С. 110390 - 110390

Опубликована: Фев. 20, 2024

Язык: Английский

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Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies

BME Frontiers, Год журнала: 2025, Номер 6

Опубликована: Янв. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Язык: Английский

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Target nucleic acid amplification-free detection of Escherichia coli O157:H7 by CRISPR/Cas12a and hybridization chain reaction based on an evanescent wave fluorescence biosensor

Sensors and Actuators B Chemical, Год журнала: 2022, Номер 376, С. 133005 - 133005

Опубликована: Ноя. 21, 2022

Язык: Английский

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Hierarchically Structured and Highly Dispersible MOF Nanozymes Combining Self-Assembly and Biomineralization for Sensitive and Persistent Chemiluminescence Immunoassay

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(36), С. 42404 - 42412

Опубликована: Авг. 29, 2023

Metal-organic frameworks (MOF) are promising candidates for the construction of artificial nanozymes and have found applications in many fields. However, preparation nanosized MOF materials with high performance good dispersibility is still a big challenge great demand as signal labels immunoassays. In this work, hierarchically structured highly dispersible nanoparticles were facilely prepared one-pot method. Self-assembled micelles from PEGylated hematin used templates to mediate formation zeolitic imidazole framework-8 (ZIF-8) aqueous solution. The encapsulation ZIF-8 produces well-dispersed generates dual-confinement effects catalytic hematin. Owing hierarchical structures, formed show enhanced peroxidase-like activity enable persistent chemiluminescence behaviors luminol system. Sandwich-type immunoassays carcinoembryonic antigen (CEA) proposed using labels, analytical performances achieved. combination self-assembly biomineralization may open new avenues development nanomaterials.

Язык: Английский

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