Talanta, Год журнала: 2024, Номер 274, С. 125921 - 125921
Опубликована: Март 28, 2024
Язык: Английский
Talanta, Год журнала: 2024, Номер 274, С. 125921 - 125921
Опубликована: Март 28, 2024
Язык: Английский
Analytical Chemistry, Год журнала: 2023, Номер 95(6), С. 3332 - 3339
Опубликована: Янв. 30, 2023
Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) introduced convert each target RNA strand into long single-stranded DNA with repeated sequences, which acted as triggers initiate transcleavage activity of CRISPR-Cas12a. activated Cas12a could cleave biotinylated linker CuS nanoparticles (NPs) inhibit binding NPs streptavidin immobilized surface microplate, strongly reduced generation Cu2+ from between AgNO3, thus efficiently suppressed CL Cu2+-luminol-H2O2 system, giving "signal off" biosensor. With multiple amplification, detection limit developed sensor for miRNA-21 reached 16 aM. In addition, this is not only suitable professional instrument but also smartphone used tool purpose portable low-cost assay. This method be specifically quite low level in human serum samples various cancer cells, indicating its potential ultrasensitive molecular diagnostics.
Язык: Английский
Процитировано
54TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 166, С. 117213 - 117213
Опубликована: Авг. 4, 2023
Язык: Английский
Процитировано
50Biosensors and Bioelectronics, Год журнала: 2023, Номер 247, С. 115929 - 115929
Опубликована: Дек. 15, 2023
Язык: Английский
Процитировано
34Biosensors and Bioelectronics, Год журнала: 2023, Номер 225, С. 115102 - 115102
Опубликована: Янв. 25, 2023
Язык: Английский
Процитировано
30Trends in Food Science & Technology, Год журнала: 2024, Номер 148, С. 104482 - 104482
Опубликована: Апрель 7, 2024
Язык: Английский
Процитировано
16Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Фев. 6, 2024
Major health events caused by pathogenic microorganisms are increasing, seriously jeopardizing human lives. Currently PCR and ITA widely used for rapid testing in food, medicine, industry agriculture. However, due to the non-specificity of amplification process, researchers have proposed combination nucleic acid technology with novel CRISPR detection, which improves specificity credibility results. This paper summarizes research progress conjunction CRISPR/Cas detection pathogens, provides a reference theoretical basis subsequent application field pathogen detection.
Язык: Английский
Процитировано
10Food Control, Год журнала: 2024, Номер 161, С. 110390 - 110390
Опубликована: Фев. 20, 2024
Язык: Английский
Процитировано
10BME Frontiers, Год журнала: 2025, Номер 6
Опубликована: Янв. 1, 2025
Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.
Язык: Английский
Процитировано
1Sensors and Actuators B Chemical, Год журнала: 2022, Номер 376, С. 133005 - 133005
Опубликована: Ноя. 21, 2022
Язык: Английский
Процитировано
38ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(36), С. 42404 - 42412
Опубликована: Авг. 29, 2023
Metal-organic frameworks (MOF) are promising candidates for the construction of artificial nanozymes and have found applications in many fields. However, preparation nanosized MOF materials with high performance good dispersibility is still a big challenge great demand as signal labels immunoassays. In this work, hierarchically structured highly dispersible nanoparticles were facilely prepared one-pot method. Self-assembled micelles from PEGylated hematin used templates to mediate formation zeolitic imidazole framework-8 (ZIF-8) aqueous solution. The encapsulation ZIF-8 produces well-dispersed generates dual-confinement effects catalytic hematin. Owing hierarchical structures, formed show enhanced peroxidase-like activity enable persistent chemiluminescence behaviors luminol system. Sandwich-type immunoassays carcinoembryonic antigen (CEA) proposed using labels, analytical performances achieved. combination self-assembly biomineralization may open new avenues development nanomaterials.
Язык: Английский
Процитировано
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