Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3332 - 3339

Published: Jan. 30, 2023

Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) introduced convert each target RNA strand into long single-stranded DNA with repeated sequences, which acted as triggers initiate transcleavage activity of CRISPR-Cas12a. activated Cas12a could cleave biotinylated linker CuS nanoparticles (NPs) inhibit binding NPs streptavidin immobilized surface microplate, strongly reduced generation Cu2+ from between AgNO3, thus efficiently suppressed CL Cu2+-luminol-H2O2 system, giving "signal off" biosensor. With multiple amplification, detection limit developed sensor for miRNA-21 reached 16 aM. In addition, this is not only suitable professional instrument but also smartphone used tool purpose portable low-cost assay. This method be specifically quite low level in human serum samples various cancer cells, indicating its potential ultrasensitive molecular diagnostics.

Language: Английский

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Recent development of chemiluminescence for bioanalysis

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 166, P. 117213 - 117213

Published: Aug. 4, 2023

Language: Английский

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Universal smartphone-assisted label-free CRISPR/Cas12a-DNAzyme chemiluminescence biosensing platform for on-site detection of nucleic acid and non-nucleic acid targets

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 247, P. 115929 - 115929

Published: Dec. 15, 2023

Language: Английский

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A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 225, P. 115102 - 115102

Published: Jan. 25, 2023

Language: Английский

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Isothermal nucleic acid amplification based microfluidic “lab-on-a-chip” for the detection of pathogenic bacteria and viruses in agri-foods

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 148, P. 104482 - 104482

Published: April 7, 2024

Language: Английский

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Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 6, 2024

Major health events caused by pathogenic microorganisms are increasing, seriously jeopardizing human lives. Currently PCR and ITA widely used for rapid testing in food, medicine, industry agriculture. However, due to the non-specificity of amplification process, researchers have proposed combination nucleic acid technology with novel CRISPR detection, which improves specificity credibility results. This paper summarizes research progress conjunction CRISPR/Cas detection pathogens, provides a reference theoretical basis subsequent application field pathogen detection.

Language: Английский

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A ratiometric electrochemical aptasensor for sensitive detection of kanamycin in food based on entropy-driven strand displacement reaction

Food Control, Journal Year: 2024, Volume and Issue: 161, P. 110390 - 110390

Published: Feb. 20, 2024

Language: Английский

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Target nucleic acid amplification-free detection of Escherichia coli O157:H7 by CRISPR/Cas12a and hybridization chain reaction based on an evanescent wave fluorescence biosensor

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 376, P. 133005 - 133005

Published: Nov. 21, 2022

Language: Английский

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Hierarchically Structured and Highly Dispersible MOF Nanozymes Combining Self-Assembly and Biomineralization for Sensitive and Persistent Chemiluminescence Immunoassay

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(36), P. 42404 - 42412

Published: Aug. 29, 2023

Metal-organic frameworks (MOF) are promising candidates for the construction of artificial nanozymes and have found applications in many fields. However, preparation nanosized MOF materials with high performance good dispersibility is still a big challenge great demand as signal labels immunoassays. In this work, hierarchically structured highly dispersible nanoparticles were facilely prepared one-pot method. Self-assembled micelles from PEGylated hematin used templates to mediate formation zeolitic imidazole framework-8 (ZIF-8) aqueous solution. The encapsulation ZIF-8 produces well-dispersed generates dual-confinement effects catalytic hematin. Owing hierarchical structures, formed show enhanced peroxidase-like activity enable persistent chemiluminescence behaviors luminol system. Sandwich-type immunoassays carcinoembryonic antigen (CEA) proposed using labels, analytical performances achieved. combination self-assembly biomineralization may open new avenues development nanomaterials.

Language: Английский

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Sensitive detection of viable salmonella bacteria based on tertiary cascade signal amplification via splintR ligase ligation-PCR amplification-CRISPR/Cas12a cleavage

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1248, P. 340885 - 340885

Published: Jan. 23, 2023

Language: Английский

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