Analytical Methods, Год журнала: 2024, Номер 16(29), С. 4917 - 4937
Опубликована: Янв. 1, 2024
DNAzyme-based biosensors for pathogen detection in colorimetric, fluorescent, and electrochemical detection.
Язык: Английский
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 3, 2025
Development of a multiplexed and sensitive biosensing platform is priority for public health security. We report micropore resistance counting based on polystyrene microsphere size-based encoding Clostridium butyricum Argonaute (CbAgo) decoding ultrasensitive detection. Initially, we constructed target DNA-modified coding system counting. Subsequently, the precise recognition cleavage capabilities guide DNA-activated CbAgo protein enable encoded system. Changes in concentration microspheres are presented as signal readout. The demonstrated excellent performance detection three mycotoxins (with sensitivity range over 4 orders magnitude reaching pg/mL level) two inflammatory markers at pg/mL. Combining enzyme by with counting, developed highly tool wide-ranging potential applications such clinical diagnosis, food safety inspection, environmental monitoring.
Язык: Английский
Процитировано
7
Small, Год журнала: 2024, Номер 20(24)
Опубликована: Март 20, 2024
Abstract The development of novel method for drug‐resistant bacteria detection is imperative. A simultaneous dual‐gene Test methicillin‐resistant Staphylococcus aureus (MRSA) developed using an Argonaute‐centered portable biosensor (STAR). This the first report concerning Argonaute‐based pathogenic detection. Simply, species‐specific mec and nuc gene are isothermally amplified loop‐mediated isothermal amplification (LAMP) technique, followed by enabled its programmable, guided, sequence‐specific recognition cleavage. With strategy, targeted nucleic acid signals dexterously converted into fluorescent signals. STAR capable detecting mecA simultaneously in a single reaction. limit 10 CFU/mL with dynamic range from to 7 CFU/mL. sample‐to‐result time <65 min. successfully adapted detect clinical samples, contaminated foods, MRSA‐infected animals. work broadens reach biosensing presents bacterial point‐of‐need (PON) platform.
Язык: Английский
Процитировано
11
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 16, 2025
Multiplexed and portable detection of small molecular food contaminants in complex matrices remains a challenge analytical chemistry. Herein, inspired by the multiplexed detection, nucleic-acid-targeting capability, programmability Thermus thermophilus argonaute (TtAgo), fingerprint recognition nanopore technology, we proposed TtAgo-assisted encoding strategy to detect three mycotoxins simultaneously (AFB1, OTA, ZEN). In this method, effective signal conversion turns into DNA reporters that were observed via technology . general, guide DNAs (gDNAs) released presence mycotoxins, followed specific cleavage mediated TtAgo release reporters. The translocation produces highly distinguishable information due favorable resolution nanopore. Owing high nanopore, method displayed low LODs (AFB1 67.18 fM, OTA 13.32 ZEN 11.69 fM) with wide linear ranges. Furthermore, specificity practical application also verified showed satisfactory results. conclusion, offers novel avenue for molecules.
Язык: Английский
Процитировано
1
Sensors and Actuators B Chemical, Год журнала: 2024, Номер 410, С. 135713 - 135713
Опубликована: Март 27, 2024
Язык: Английский
Процитировано
9
Advanced Healthcare Materials, Год журнала: 2024, Номер 13(18)
Опубликована: Март 26, 2024
Argonaute (Ago) as a powerful enzyme has provided new insights into biosensing due to its programmability, high sensitivity, and user-friendly operation. However, current strategies mainly rely on phosphorylated guide DNA modulate the cleavage activity of Ago, which is limited in versatility simplicity. Herein, authors report Mn
Язык: Английский
Процитировано
8
Chemical Engineering Journal, Год журнала: 2024, Номер 495, С. 153434 - 153434
Опубликована: Июль 1, 2024
Язык: Английский
Процитировано
8
Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(30), С. 16569 - 16582
Опубликована: Июль 20, 2024
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Процитировано
7
Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104882 - 104882
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
ACS Sensors, Год журнала: 2024, Номер 9(11), С. 5665 - 5682
Опубликована: Ноя. 11, 2024
Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.
Язык: Английский
Процитировано
6
Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(19), С. 11195 - 11204
Опубликована: Апрель 2, 2024
Genetically modified crops (GMCs) have been discussed due to unknown safety, and thus, it is imperative develop an effective detection technology. CRISPR/Cas deemed a burgeoning technology for nucleic acid detection. Herein, we developed novel method the first time, which combined thermostable Cas12b with loop-mediated isothermal amplification (LAMP), detect genetically (GM) soybeans in customized one-pot vessel. In our method, LAMP-specific primers were used amplify cauliflower mosaic virus 35S promoter (CaMV35S) of GM soybean samples. The corresponding amplicons activated
Язык: Английский
Процитировано
5