Buoyant Metal–Organic Framework Corona-Driven Fast Isolation and Ultrasensitive Profiling of Circulating Extracellular Vesicles

ACS Nano, Год журнала: 2024, Номер 18(22), С. 14569 - 14582

Опубликована: Май 23, 2024

Accurately assaying tumor-derived circulating extracellular vesicles (EVs) is fundamental in noninvasive cancer diagnosis and therapeutic monitoring but limited by challenges efficient EV isolation profiling. Here, we report a bioinspired buoyancy-driven metal-organic framework (MOF) corona that leverages on-bubble coordination dual-encoded surface-enhanced Raman scattering (SERS) nanotags to streamline rapid ultrasensitive profiling of plasma EVs single assay for diagnostics. This integrated bubble-MOF-SERS (IBMsv) allows barnacle-like high-density adhesion MOFs on self-floating bubble surface enable fast (2 min, near 90% capture efficiency) tumor via enhanced EV-MOF binding. Also, IBMsv harnesses four-plexed SERS profile the captured protein markers at single-particle level. Such sensitive multiplexed across five types, revealing heterogeneous expression patterns. Furthermore, enables pilot clinical cohort (

Язык: Английский

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Understanding the Electrochemical MOF Sensors in Detecting Cancer with Special Emphasis on Breast Carcinoma Biomarkers

Topics in Current Chemistry, Год журнала: 2025, Номер 383(1)

Опубликована: Фев. 18, 2025

Язык: Английский

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Advances in magnetic affinity-based isolation/detection of exosomes for robust diagnostics

Microchimica Acta, Год журнала: 2025, Номер 192(4)

Опубликована: Март 5, 2025

The review article provides a short introduction to exosomes with the focus use as disease markers itself (i.e. their concentration or presence of some specific receptors) source biomarkers such proteins and metabolites. In detail, we are discussing various methods exosome isolation main paper is on affinity capture exosomes, since them can be applied sub-populations produced by organs. comprehensive overview magnetic (bio)affinity detection exosomal cargo using different ligands antibodies, DNA aptamers, peptides, glycan-based recognition, transferrin-based approaches, based recognition phospholipids other approaches including electrostatic interactions. in detail key analytical clinical parameters form an extensive table summarising outcomes published last two years (2023-2024). Finally, also conclusions sections pros cons for and/or determination content.

Язык: Английский

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Advances in MOFs-based microneedles in the treatment of skin diseases

Applied Materials Today, Год журнала: 2025, Номер 42, С. 102616 - 102616

Опубликована: Янв. 31, 2025

Язык: Английский

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Dual-target magneto-immunoassay with bifunctional nanohybrids for breast cancer exosome detection

Talanta, Год журнала: 2025, Номер 286, С. 127532 - 127532

Опубликована: Янв. 8, 2025

Язык: Английский

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Biosensor-based methods for exosome detection with applications to disease diagnosis

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117362 - 117362

Опубликована: Март 1, 2025

Язык: Английский

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A trifunctional surface ligand-directed general synthesis of 2D MOF hybrid nanozymes for customizable applications

Journal of Colloid and Interface Science, Год журнала: 2025, Номер 691, С. 137453 - 137453

Опубликована: Март 28, 2025

Язык: Английский

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Proximity-induced amplification cascade for accurate and sensitive colorimetric detection of tumor-derived exosomes

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137832 - 137832

Опубликована: Апрель 1, 2025

Язык: Английский

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All-in-one detection of breast cancer-derived exosomal miRNA on a pen-based paper chip

The Analyst, Год журнала: 2024, Номер 149(4), С. 1250 - 1261

Опубликована: Янв. 1, 2024

An all-in-one detection strategy was proposed for the identification of breast cancer-derived exosomal miRNA-21, exhibiting high sensitivity and specificity on a pen-based paper chip.

Язык: Английский

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Integrating All-rounder TiO₂ Accelerated Electrochemiluminescence with Dual-Quenching PDA@COF Probes for Sensitive Quantification and Protein Profiling of Tumorous Exosomes

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(45), С. 61692 - 61702

Опубликована: Ноя. 4, 2024

Exosomes have been perceived as promising biomarkers for noninvasive cancer diagnosis and treatment monitoring. However, the sensitive accurate quantification phenotyping of exosomes remains challenging. Herein, a versatile electrochemiluminescence (ECL) aptasensor was proposed analysis tumorous exosomes. Specifically, ternary nanohybrid (Ru-HAuTiO2), by covalently linking ECL luminophore Ru(dcbpy)32+ with gold nanoparticles (AuNPs)-decorated hollow urchin-like TiO2 (HTiO2), ingeniously designed highly luminescent self-enhanced nanoemitter. Notably, porous HTiO2 played an "all-rounder" role, including carrier luminophores AuNPs, coreaction accelerator, specific exosome capturing scaffold through Ti–phosphate coordination interaction. On other hand, polydopamine modified covalent organic framework (PDA@COF) employed quencher to remarkably attenuate Ru-HAuTiO2 dual-quenching mechanism, further labeled aptamer (Apt) exosomal surface protein. Based on forming Ru-HAuTiO2/exosome/Apt-PDA@COF sandwich structure electrode, "signal on–off" platform constructed, realizing detection within range 3.1 × 103 particles/mL 1 108 low limit 1.41 particles/mL, achieving phenotypic profiling proteins different This work provides alternative method

Язык: Английский

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