ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(42), С. 56874 - 56883
Опубликована: Окт. 11, 2024
As
an
emerging
source
for
liquid
biopsy,
exosomes
hold
significant
promise
clinical
diagnosis.
However,
commonly
used
exosome
isolation
methods
(e.g.,
ultracentrifugation)
suffer
from
low
throughput
a
large
number
of
samples.
Herein,
dysprosium–metal
organic
framework
was
synthesized
and
doped
with
nanofibers
by
electrospinning
efficient
capture
body
fluid.
With
the
integration
multichannel
pipet
or
robot
automatic
workstation,
high
can
be
achieved
samples
reproducibility.
To
evaluate
value
developed
method,
urinary
were
enriched
34
liver
disease
different
stages
profiling
metabolites
mass
spectrometry.
The
results
showed
that
HCC,
cirrhosis,
healthy
controls
significantly
differentiated
Random
Forest
classification
model.
has
promising
applications
in
exosome-based
biopsy
large-scale
Analytical Chemistry,
Год журнала:
2024,
Номер
96(46), С. 18555 - 18563
Опубликована: Ноя. 5, 2024
In
this
study,
we
developed
ratiometric
surface-enhanced
Raman
scattering
(SERS)
biosensors
using
Ag-Au
alloy
nanoflowers
as
SERS
substrates,
molecules
having
amide
bonds
and
alkyne
groups
(Tag
A)
reporters,
sodium
thiocyanate
an
internal
standard
molecule
B)
for
the
sensitive
detection
of
human
carboxylesterase-1
(hCE1)
in
HepG-2
cells.
The
correlation
between
cell
damage
hCE1
activity
levels
was
investigated.
Both
Tag
A's
group
B's
cyanide
produced
characteristic
signals
Raman-silent
region
(
Abstract
For
every
epidemic
outbreak,
the
prevention
and
treatments
in
resource‐limited
areas
are
always
out
of
reach.
Critical
to
this
is
that
high
accuracy,
stability,
more
comprehensive
analytical
techniques
rely
on
expensive
bulky
instruments
large
laboratories.
Here,
a
fully
integrated
high‐throughput
microfluidic
system
proposed
for
ultra‐multiple
point‐of‐care
immunoassay,
termed
Dac
system.
Specifically,
only
requires
handheld
portable
device
automatically
recycle
repetitive
multi‐step
reactions
including
on‐demand
liquid
releasing,
dispensing,
metering,
collecting,
oscillatory
mixing,
discharging.
The
performs
high‐precision
enzyme‐linked
immunosorbent
assays
up
17
samples
or
targets
simultaneously
single
chip.
Furthermore,
reagent
consumption
2%
compared
conventional
ELISA,
microbubble‐accelerated
shorten
assay
time
by
than
half.
As
proof
concept,
multiplexed
detections
achieved
detecting
at
least
four
infection
two
singular
barcode‐based
multi‐target
results
can
rapidly
distinguish
between
five
similar
cases,
allowing
accurate
therapeutic
interventions.
Compared
clinical
instruments,
accuracy
inflammation
classification
92.38%
(
n
=
105),
with
quantitative
correlation
coefficient
R
2
0.9838,
while
specificity
100%
sensitivity
98.93%.
Abstract
Solid
bubbles
have
expanded
the
SERS
assay
toolbox,
but
their
detection
performance
in
biofluids
is
still
hampered
by
irrational
design
of
plasmonic
sensing
interface.
A
bubble
aggregate‐driven
DNA‐encoded
reported
here
that
enables
simultaneous,
ultrasensitive,
and
specific
multiple
miRNAs
blood
samples
for
accurate
cancer
diagnosis.
In
this
assay,
buoyancy
allows
them
to
self‐aggregate
at
a
droplet
apex
reconfiguration,
form
single‐layer
aggregates
with
nanogaps,
prevent
coffee
ring
effect
during
evaporation
assembly.
Furthermore,
seamlessly
couple
dual‐color
catalytic
hybridization
assembly
amplify
miRNA‐responsive
Raman
signal,
function
as
both
an
analyte
concentrator
signal
aggregator
without
external
forces.
Using
these
merits,
magnet‐free,
portable
achieves
femtomolar
dual‐miRNA
quantitation
single‐base
resolution,
simultaneous
miRNA
across
four
cell
lines,
diagnosis
(AUC
=
1)
via
analyzing
40
machine
learning,
thus
providing
promising
tool
clinical
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
The
unmet
on‐site
clinical
diagnostics
are
increasingly
demanding
portable
bioassays,
but
compromised
by
sensitivity
and
throughput.
Herein,
a
nanostructured
bubbles‐enhanced
fluorescence
(nBEF)
assay
is
reported
interfacing
with
smartphone
imaging
system
to
enable
portable,
ultrasensitive,
specific
miRNA
detection
in
parallel.
In
nBEF,
the
high‐curvature
bubbles
allow
physically
enhancing
optical
propagation,
chemically
fluorescence,
assembling
more
DNA
probes,
undergoing
faster
self‐suspension
efficient
self‐aggregation
enhancement
relative
their
smooth
counterparts.
By
coupling
bubble‐driven
signal
amplification
plug‐and‐play
concatemer,
system,
this
nBEF
achieves
ultrasensitive
fluorescent
of
at
femtomolar
level
one‐base
resolution
single
test,
capable
monitoring
drug‐induced
dynamic
expression
accurate
cancer
diagnosis
(AUC
=
1)
through
profiling
40
plasma
samples
after
RNA
extraction.
This
further
integrates
an
R‐value
analysis
RGB
module
realize
automated,
readout.
Such
high‐performance
provides
low‐cost,
highly
sensitive
tool
diagnosis.
