Sophorolipids: A comprehensive review on properties and applications

Advances in Colloid and Interface Science, Год журнала: 2023, Номер 313, С. 102856 - 102856

Опубликована: Фев. 9, 2023

Язык: Английский

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Sustainable biosurfactant production from secondary feedstock—recent advances, process optimization and perspectives

Frontiers in Chemistry, Год журнала: 2024, Номер 12

Опубликована: Янв. 19, 2024

Biosurfactants have garnered increased attention lately due to their superiority of properties over fossil-derived counterparts. While the cost production remains a significant hurdle surpass synthetic surfactants, biosurfactants been anticipated gain larger market share in coming decades. Among these, glycolipids, type low-molecular-weight biosurfactant, stand out for efficacy reducing surface and interfacial tension, which made them highly sought-after various surfactant-related applications. Glycolipids are composed hydrophilic carbohydrate moieties linked hydrophobic fatty acid chains through ester bonds that mainly include rhamnolipids, trehalose lipids, sophorolipids, mannosylerythritol lipids. This review highlights current landscape glycolipids covers specific glycolipid productivity diverse range products found global market. Applications such as bioremediation, food processing, petroleum refining, biomedical uses, increasing agriculture output discussed. Additionally, latest advancements reduction challenges utilizing second-generation feedstocks sustainable also thoroughly examined. Overall, this proposes balance between environmental advantages, economic viability, societal benefits optimized integration secondary biosurfactant production.

Язык: Английский

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Bacillus licheniformis: The unexplored alternative for the anaerobic production of lipopeptide biosurfactants?

Biotechnology Advances, Год журнала: 2022, Номер 60, С. 108013 - 108013

Опубликована: Июнь 23, 2022

Microbial biosurfactants have attracted the attention of researchers and companies for last decades, as they are considered promising candidates to replace chemical surfactants in numerous applications. Although years, considerable advances were performed regarding strain engineering use low-cost substrates order reduce their production costs, one main bottlenecks is at industrial scale. Conventional aerobic biosurfactant processes result excessive foaming, due high agitation aeration rates necessary increase dissolved oxygen concentration allow microbial growth production. Different approaches been studied overcome this problem, although with limited success. A not widely explored alternative development foam-free through anaerobic biosurfactant-producing microorganisms. Surfactin, produced by Bacillus subtilis, most lipopeptide biosurfactant, powerful known so far. licheniformis strains produce lichenysin, a which structure similar surfactin. However, despite its extraordinary surface-active properties potential applications, lichenysin has scarcely studied. According previous studies, B. better adapted than could be good biosurfactants. In review, limitations surfactin under conditions will analyzed, possibility implementing production, expand market applications different fields, discussed.

Язык: Английский

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Sophorolipids—Bio-Based Antimicrobial Formulating Agents for Applications in Food and Health

Molecules, Год журнала: 2022, Номер 27(17), С. 5556 - 5556

Опубликована: Авг. 29, 2022

Sophorolipids are well-known glycolipid biosurfactants, produced mainly by non-pathogenic yeast species such as Candida bombicola with high yield. Its unique environmental compatibility and biodegradable properties have made them a focus in the present review for their promising applications diverse areas. This study aims to examine current research trends of sophorolipids evaluate food health. A literature search was conducted using different databases including PubMed, ScienceDirect, EBSCOhost, Wiley Online Library identify studies on fundamental mechanisms Studies shown that various structural forms exhibit biological physicochemical properties. represent one most attractive biosurfactants industry due antimicrobial action against both Gram-positive Gram-negative microorganisms health sectors. In this review, we provided an overview detailed analysis areas food, agriculture, pharmaceutical, cosmetic, anticancer, activities.

Язык: Английский

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Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Molecules, Год журнала: 2022, Номер 27(15), С. 5037 - 5037

Опубликована: Авг. 8, 2022

Biosurfactants with anti-inflammatory activity may alleviate skin irritation caused by synthetic surfactants in cleaning products. Sophorolipid (SL) is a promising alternative to surfactants. However, there are few reports on the of SL and underlying mechanism. The purpose this work verify that lipopolysaccharide (LPS)-induced inflammation could be inhibited through targeting pathway nuclear factor-κB (NF-κB) RAW264.7 cells.The influence cytokine release was investigated LPS-induced cells using ELISA. quantification protein expression corresponding molecular markers realized Western blot analysis. Flow cytometry employed determine levels Ca2+ reactive oxygen species (ROS). relative inducible nitric oxide synthase (INOS) cyclooxygenase-2 (COX-2) determined RT-PCR. An immunofluorescence assay confocal microscope were used observe NF-κB/p65 translocation from cytoplasm into nucleus. likely targets predicted docking analysis.SL showed reduced inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), (NO). experimental results show suppressed ROS influx alleviated iNOS COX-2, NF-κB (p65) nucleus, phosphorylated proteins such as p65 IκBα. Furthermore, analysis inhibit signaling competing LPS bind TLR4/MD-2 hydrophobic interactions inhibiting IKKβ activation hydrogen bonding interactions.This study demonstrated exerted via cells.

Язык: Английский

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Agricultural Bioinputs Obtained by Solid-State Fermentation: From Production in Biorefineries to Sustainable Agriculture

Sustainability, Год журнала: 2024, Номер 16(3), С. 1076 - 1076

Опубликована: Янв. 26, 2024

Agriculture plays a major role on society, especially in developing countries which rely commodity exportation markets. To maintain high crop productivity, the use of agrochemicals was once employed as main strategy, turn affected soil, water, and human health. In order to aid this issue, identifying some alternatives, such implementation biofertilizers inoculants bioinputs modern agriculture, are imperative improve ecosystem quality. Among these bioinputs, few bioproducts have shown good performances, phytohormones (e.g., auxins giberellins), biosurfactants, other enzymes; thus, it is extremely important assure quality feasibility their production biorefinery scenarios. These can be synthesized through fermentation processes utilizing plant biomasses agricultural byproducts carbon sources. sense, increase tecno-economical availability processes, solid-state (SSF) has great potential due its ease operation cost-attractiveness. Therefore, study aims describe substrates used SSF systems for bioinputs; associated hurdles, parameters, conditions selection; most suitable microorganisms; underlying mechanisms molecules soil dynamics. Within context, expected contribute development new biorefineries mitigation environmental impacts.

Язык: Английский

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Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Апрель 27, 2024

Abstract As a kind of biosurfactants, iturin A has attracted people’s wide attentions due to their features biodegradability, environmentally friendly, etc.; however, high production cost limited its extensive application, and the aim this research wants improve in Bacillus amyloliquefaciens . Firstly, dual promoter was applied strengthen synthetase expression, yield increased 1.25 g/L. Subsequently, original 5′-UTRs downstream genes ( ituA , ituB ituC ) cluster were optimized, which significantly mRNA secondary stability, produced by resultant strain HZ-T3 reached 2.32 Secondly, synthetic pathway α-glucosidase inhibitor 1-deoxynojirimycin blocked substrate corn starch utilization, 34.91% 3.13 Thirdly, efficient precursor (fatty acids, Ser, Pro) supplies proven as critical role synthesis, 5.52 g/L attained strain, through overexpressing yngH serC introducing ocD Meanwhile, responsible for poly-γ-glutamic acid, extracellular polysaccharide, surfactin syntheses deleted, led 30.98% increase yield. Finally, lipopeptide transporters screened, 17.98% SwrC overexpression 8.53 g/L, is highest ever reported. This study laid foundation industrial application development A, provided guidance metabolic engineering breeding other metabolites synthesized non-ribosomal peptide synthetase. Key points • Optimizing 5′-UTR an effective tactics regulate expression. Blocking 1-DNJ synthesis benefited utilization production. The work reported so far.

Язык: Английский

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Enhanced penetration and biofilm eradication by sophorolipid micelles encapsulating Honokiol: a comprehensive solution for biofilm-associated lung infections

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 3, 2025

Biofilm-associated lung infections, particularly those caused by Staphylococcus aureus (S. aureus), pose significant clinical challenges to conventional therapies. S. Biofilm infections are refractory treatment due the presence of persister bacterial cells and barrier effect unique extracellular polymeric substances (EPS). This study describes development multifunctional micelles, HK-SL Ms, utilizing sophorolipid (SL) encapsulate Honokiol (HK). Ms potently disrupted EPS barrier, killed some internal colonizing bacteria, inhibited further adhesion. Consequently, dynamic cycling biofilms was hindered, achieving a promising removal biofilms. In vitro studies demonstrated that exhibited antimicrobial reduction 6.42 log10CFU/mL. eradicated 71.73% targeting polysaccharides, proteins, viable within biofilm. Additionally, 1.66 log10CFU/mL units were killed. Moreover, 91.10% early biofilm formation obstructing initial adhesion polysaccharides polysaccharide intercellular adhesins (PIA). Thus, reestablishment reinfection could be resolved promisingly. as predominant in acute pneumonia chronic cases, inducing similar inflammation. murine model infected aureus, significantly reduced load lungs, decreased inflammatory factor levels, repaired tissue damage. offers novel strategy for biofilm-associated dispersing removing preventing new infections.

Язык: Английский

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Sophorolipids bioproduction in the yeast Starmerella bombicola: Current trends and perspectives

Bioresource Technology, Год журнала: 2021, Номер 346, С. 126593 - 126593

Опубликована: Дек. 20, 2021

Язык: Английский

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Solid-State Fermentation from Organic Wastes: A New Generation of Bioproducts

Processes, Год журнала: 2022, Номер 10(12), С. 2675 - 2675

Опубликована: Дек. 12, 2022

Solid-state fermentation (SSF) is part of the pathway to consolidate waste as a relevant alternative for valorization organic waste. The objective SSF produce one or several bioproducts added value from solid substrates. can use wide variety substrates thus, it an excellent candidate in framework circular bioeconomy change status feedstock. development was boosted previous decade by scientific efforts devoted production hydrolytic enzymes. Nowadays, has expanded other valuable products: biosurfactants, biopesticides, aromas, pigments, and bio-flocculants, among others. This review explores conditions obtain main emerging products highlight discuss challenges related scale-up these processes downstream, which hamper their further commercialization.

Язык: Английский

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