ACS Biomaterials Science & Engineering,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 16, 2025
Osteoarthritis
(OA)
is
a
chronic
multifactorial
disease
characterized
by
cartilage
degeneration,
pain,
and
reduced
mobility.
Current
therapies
primarily
aim
to
relieve
pain
restore
function,
but
they
often
have
limited
effectiveness
side
effects.
Coixol,
bioactive
compound
from
Coix
lacryma-jobi
L.,
exhibits
anti-inflammatory
analgesic
properties,
suggesting
potential
benefits
in
OA
treatment.
This
study
explored
the
effects
of
coixol
on
chondrocytes.
Primary
chondrocytes
rats
were
isolated
treated
with
varying
concentrations
coixol.
Cell
viability
proliferation
assessed
using
CCK-8
assays.
The
expression
genes
related
ferroptosis
autophagy
was
analyzed
through
RT-qPCR,
Western
blot,
immunofluorescence.
Moreover,
investigated
characteristics
performance
coixol-loaded
PDLLA–PEG-PDLLA
(PLEL)/gelatin
sponge
(GS)
hydrogels
(Coixol@PLEL/GS)
for
enhancing
osteochondral
defect
repair
specifically
targeting
chondrocyte
autophagy.
PDLLA–PEG-PDLLA/gelatin
evaluated
cryo-scanning
electron
microscopy
(SEM)
or
SEM,
release
kinetics
determined.
In
vivo,
rat
model
used
assess
efficacy
Coixol@PLEL/GS
International
Cartilage
Repair
Society
(ICRS)
scores,
Safranin
O/Fast
green
staining,
Toluidine
blue
Coixol
significantly
increased
dose-dependent
manner.
Furthermore,
inhibited
stimulated
autophagy,
as
evidenced
upregulation
genes.
remarkably
enhanced
defects
compared
that
control
groups.
conclusion,
protects
improving
survival,
inhibiting
ferroptosis,
activating
highlighting
its
therapeutic
strategy
Microbial Cell Factories,
Год журнала:
2025,
Номер
24(1)
Опубликована: Янв. 10, 2025
Abstract
Background
Paenibacillus
polymyxa
,
is
a
Gram-positive,
plant
growth
promoting
bacterium,
known
for
producing
98%
optically
pure
2,3-butanediol,
an
industrially
valuable
chemical
solvents,
plasticizers
and
resins.
Immobilization
of
has
been
proposed
to
improve
the
cell
stability
efficiency
fermentation
process,
reduce
contamination
provide
easy
separation
butanediol
in
culture
broth
as
compared
conventional
bioprocesses.
This
research
aimed
explore
potential
with
immobilization
technique
produce
2,3-butanediol.
Results
We
investigated
different
methods
natural
biopolymers
like
alginate,
chitosan
carrageenan-chitosan-based
immobilization.
These
were
further
their
yield
2,3-butanediol
production.
Carrageenan-chitosan
beads
enabled
higher
concentration
demonstrated
superior
retention
calcium-alginate-chitosan
beads.
preserved
production
bacteria
increased
product
formation
rate.
Conclusion
enables
non-pathogenic
be
capable
producer
rate,
which
not
previously
reported.
novel
strategy
offers
promising
alternative
traditional
processes
using
pathogenic
strains
can
applied
co-cultivations
metabolite
production,
wastewater
management
bioremediation.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(22), С. 5360 - 5376
Опубликована: Янв. 1, 2024
Articular
cartilage
tissue
has
limited
self-repair
capabilities,
with
damage
frequently
progressing
to
irreversible
degeneration.
Engineered
tissues
constructed
through
bioprinting
and
embedded
stem
cell
aggregates
offer
promising
therapeutic
alternatives.
Aggregates
of
bone
marrow
mesenchymal
stromal
cells
(BMSCs)
demonstrate
enhanced
more
rapid
chondrogenic
differentiation
than
isolated
cells,
thus
facilitating
repair.
However,
it
remains
a
key
challenge
precisely
control
biochemical
microenvironments
regulate
cellular
adhesion
cohesion
within
bioprinted
matrices
simultaneously.
Herein,
this
work
reports
bioprintable
hydrogel
matrix
high
aggregation
properties
for
The
comprises
an
cell-adhesive
gelatin
methacrylate
cell-cohesive
chitosan
(CHMA),
both
which
are
subjected
photo-initiated
crosslinking.
By
adjusting
the
CHMA
content,
mechanical
stability
cues
hydrogels
finely
tuned
promote
aggregation,
repair
implantation.
Multi-layer
constructs
encapsulated
BMSCs,
viability
reaching
91.1%,
photo-crosslinked
support
21
days.
BMSCs
rapidly
form
display
efficient
on
constructs,
as
evidenced
by
upregulated
expression
Sox9,
Aggrecan
Collagen
2a1
genes,
along
protein
levels.
Transplantation
these
BMSC-laden
into
cartilaginous
defects
demonstrates
effective
hyaline
Overall,
cell-responsive
scaffold
holds
immense
promise
applications
in
engineering.
Polymers,
Год журнала:
2024,
Номер
16(13), С. 1937 - 1937
Опубликована: Июль 6, 2024
Over
millions
of
years
evolution,
nature
has
developed
a
myriad
unique
features
that
have
inspired
the
design
adhesives
for
wound
healing.
Bionic
hydrogel
adhesives,
capable
adapting
to
dynamic
movements
tissues,
possess
superior
biocompatibility
and
effectively
promote
healing
both
external
internal
wounds.
This
paper
provides
systematic
review
principles
these
focusing
on
treatment
skin
wounds,
explores
feasibility
incorporating
nature-inspired
properties
into
their
design.
The
adhesion
mechanisms
bionic
are
analyzed
from
chemical
physical
perspectives.
Materials
natural
synthetic
polymers
commonly
used
as
detailed
regarding
degradability.
multifunctional
elements
trauma
treatment,
such
self-healing,
drug
release,
responsive
design,
optimization
mechanical
properties,
further
explored.
aim
is
overcome
limitations
conventional
treatments
offer
safer,
more
effective
solution
application
dressings.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Ноя. 9, 2024
Since
wound
healing
is
one
of
the
most
important
medical
challenges
and
common
dressings
have
not
been
able
to
manage
this
challenge
well
today,
efforts
increased
achieve
an
advanced
dressing.
Mesenchymal
stem
cells
exosomes
derived
from
them
shown
high
potential
in
regenerating
wounds
due
their
immunomodulatory,
anti-inflammatory,
immunosuppressive,
regenerative
capacities.
However,
such
as
short
life
these
cells,
low
durability
area,
stability
resulted
limitations
use
for
healing.
Nowadays,
different
scaffolds
are
considered
suitable
biomaterials
These
made
natural
or
synthetic
polymers
promising
ideal
dressing
that
does
disadvantages
dressings.
One
strategies
has
attracted
much
attention
today
using
seeding
delivering
MSCs
exosomes.
This
combined
strategy
a
enhancing
shelf
increasing
In
review,
combination
with
comprehensively
discussed.
World Journal of Stem Cells,
Год журнала:
2024,
Номер
16(4), С. 334 - 352
Опубликована: Апрель 24, 2024
Wound
repair
is
a
complex
challenge
for
both
clinical
practitioners
and
researchers.
Conventional
approaches
wound
have
several
limitations.
Stem
cell-based
therapy
has
emerged
as
novel
strategy
to
address
this
issue,
exhibiting
significant
potential
enhancing
healing
rates,
improving
quality,
promoting
skin
regeneration.
However,
the
use
of
stem
cells
in
regeneration
presents
challenges.
Recently,
biomaterials
been
identified
crucial
components
wound-healing
process.
Combination
involving
development
biocompatible
scaffolds,
accompanying
cells,
multiple
biological
factors,
structures
resembling
natural
extracellular
matrix
(ECM)
gained
considerable
attention.
Biological
scaffolds
encompass
range
that
serve
platforms
seeding
providing
them
with
an
environment
conducive
growth,
similar
ECM.
These
facilitate
delivery
application
tissue
healing.
This
article
provides
comprehensive
review
current
developments
applications
healing,
emphasizing
their
capacity
cell
adhesion,
proliferation,
differentiation,
paracrine
functions.
Additionally,
we
identify
pivotal
characteristics
contribute
enhanced
cellular
activity.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(25), С. 6005 - 6032
Опубликована: Янв. 1, 2024
Periodontitis
is
an
immune-inflammatory
disease
caused
by
dental
plaque,
and
deteriorates
the
periodontal
ligament,
causes
alveolar
bone
loss,
may
lead
to
tooth
loss.
To
treat
periodontitis,
antibacterial
anti-inflammation
approaches
are
required
reduce
Thus,
appropriate
drug
administration
methods
significant.
Due
their
"syringeability",
biocompatibility,
convenience,
injectable
hydrogels
associated
have
been
extensively
studied
used
for
periodontitis
therapy.
Such
made
from
natural
synthetic
polymer
materials
using
physical
and/or
chemical
cross-linking
approaches.
Interestingly,
some
stimuli-responsive
hydrogels,
which
respond
local
microenvironment
form
that
release
drugs.
Therefore,
as
different
highly
varied,
we
systematically
reviewed
treatment
field
three
perspectives:
raw
material
sources,
methods,
methods.
We
then
discussed
current
challenges
opportunities
translation
of
clinic,
guide
further
hydrogel
designs
periodontitis.
