Genetically encoded horse heart myoglobin for electron microscopy imaging in mammalian cells
Chemical Engineering Journal,
Год журнала:
2025,
Номер
unknown, С. 161854 - 161854
Опубликована: Март 1, 2025
Язык: Английский
Recent advances in correlative cryo-light and electron microscopy
Current Opinion in Structural Biology,
Год журнала:
2024,
Номер
89, С. 102934 - 102934
Опубликована: Окт. 3, 2024
Язык: Английский
FLEX: genetically encodable enzymatic fluorescence signal amplification using engineered peroxidase
Cell chemical biology,
Год журнала:
2024,
Номер
31(3), С. 502 - 513.e6
Опубликована: Март 1, 2024
Язык: Английский
Electron Transfer Drives the Photosensitized Polymerization of Contrast Agents by Flavoprotein Tags for Correlative Microscopy
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(34), С. 23797 - 23805
Опубликована: Авг. 16, 2024
Singlet
oxygen
generation
has
long
been
considered
the
key
feature
that
allows
genetically
encoded
fluorescent
tags
to
produce
polymeric
contrast
agents
for
electron
microscopy.
Optimization
of
singlet
sensitization
quantum
yield
not
included
effects
electron-rich
monomers
on
sensitizer's
photocycle.
We
report
at
monomer
concentrations
employed
staining,
quenching
by
transfer
is
primary
deactivation
pathway
photoexcitations.
A
simple
photochemical
model
including
contributions
from
both
processes
reproduces
observed
reaction
rates
and
indicates
most
product
driven
pathways
involve
with
monomers─not
oxygen.
Realizing
importance
these
competing
offers
a
new
paradigm
guide
development
encodable
suggests
opportunities
expand
materials
scope
growth
conditions
(e.g.,
biocompatible
monomers,
O
Язык: Английский
Engineering of LOV-domains for their use as protein tags
Archives of Biochemistry and Biophysics,
Год журнала:
2024,
Номер
unknown, С. 110228 - 110228
Опубликована: Ноя. 1, 2024
Язык: Английский
Fe-TAMLs as a new class of small molecule peroxidase probes for correlated light and electron microscopy
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 26, 2023
ABSTRACT
We
introduce
Fe-TAML,
a
small
molecule-based
peroxidase
as
versatile
new
member
of
the
correlated
fluorescence
and
electron
microscopy
toolkit.
The
utility
probe
is
demonstrated
by
high
resolution
imaging
newly
synthesized
DNA
(through
biorthogonal
labeling),
genetically
tagged
proteins
(using
HaloTag),
untagged
endogenous
(via
immunostaining).
EM
visualization
in
these
applications
facilitated
exploiting
Fe-TAML’s
catalytic
activity
for
deposition
localized
osmiophilic
precipitates
based
on
polymerized
3,3’-diaminobenzidine.
Optimized
conditions
synthesizing
implementing
Fe-TAML
probes
are
also
described.
Overall,
chemical
biology
tool
that
can
be
used
to
visualize
diverse
biomolecular
species
along
nanometer
micron
scales
within
cells.
Язык: Английский