Coumarin-based near-infrared fluorogenic probes: Recent advances, challenges and future perspectives

Coordination Chemistry Reviews, Год журнала: 2023, Номер 480, С. 215020 - 215020

Опубликована: Янв. 25, 2023

Язык: Английский

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A Lipid Droplet-Specific NIR Fluorescent Probe with a Large Stokes Shift for In Vivo Visualization of Polarity in Contrast-Induced Acute Kidney Injury

Analytical Chemistry, Год журнала: 2023, Номер 95(31), С. 11785 - 11792

Опубликована: Июль 7, 2023

The research on lipid droplets (LDs) has attracted great attention in the field of biomedical science recent years. LD malfunction is found to be associated with development acute kidney injury (AKI). To monitor this biological process and explain related pathological behavior, excellent fluorescent probes a polarity-sensitive character would provide desirable strategy. Herein, we designed new polarity-susceptible probe named LD-B targetability, which exhibits very weak fluorescence highly polar solvents based twisted intramolecular charge transfer effect but enhanced low environments, enabling us visualize polarity alteration. also possesses merits intense near-infrared (NIR) emission, good photostability, large Stokes shift, toxicity, faster metabolic rate, wash-free ability; thereby, it contribute efficient visualization application. Using via confocal laser scanning imaging small-animal system vivo, first manifested prominent rise contrast-induced AKI (CI-AKI), not only at cellular level animals vivo. Furthermore, vivo studies suggest that could accumulate kidney. In addition, normal cell lines (including cells) exhibiting greater LDs than cancer cells have been demonstrated systemically. Altogether, our work presents an effective approach for medical diagnosis CI-AKI identification potential therapeutic markers.

Язык: Английский

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Recent advances in small-molecule fluorescent probes for diagnosis of cancer cells/tissues

Coordination Chemistry Reviews, Год журнала: 2022, Номер 477, С. 214946 - 214946

Опубликована: Дек. 9, 2022

Язык: Английский

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Fluorescence Imaging of Intracellular Glutathione Levels in the Endoplasmic Reticulum to Reveal the Inhibition Effect of Rutin on Ferroptosis

Analytical Chemistry, Год журнала: 2023, Номер 95(3), С. 1949 - 1957

Опубликована: Янв. 9, 2023

Ferroptosis is an emerging form of nonapoptotic cell death, and the search for novel ferroptosis inhibitors great importance to explore unique cytoprotective strategies against ferroptosis-relevant diseases. In this work, we present endoplasmic reticulum-targeting fluorescent probe (ER-G) imaging intracellular glutathione (GSH) levels revealed inhibition effect rutin on ferroptosis. Structurally, ER-G utilized a cyclohexyl sulfonylurea as unit, single-crystal X-ray diffraction analysis confirmed that possessed N-oxide pyridine sulfinyl group instead sulfone. After response GSH, fluorescence intensity at 523 nm displayed significant increase by 3900-fold. showed extreme sensitivity selectivity GSH. The results demonstrated exhibited excellent properties could be applied monitor GSH in reticulum during erastin-induced process. By reticulum, it was efficiently block depletion potentially act inhibitor. Moreover, unlike traditional inhibitors, speculated mechanism integration chelate Fe(II) ions antioxidant effect. We expect provide convenient feasible method evaluate small molecules

Язык: Английский

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Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

Analytical Chemistry, Год журнала: 2023, Номер 95(11), С. 5034 - 5044

Опубликована: Март 10, 2023

Adenosine triphosphate (ATP), as an important intracellular energy currency produced in mitochondria, is closely related to various diseases living organisms. Currently, the biological application of AIE fluorophore a fluorescent probe for ATP detection mitochondria rarely reported. Herein, D−π–A and D–A structure-based tetraphenylethylene (TPE) fluorophores were employed synthesize six different probes (P1–P6), phenylboronic acid groups dual positive charge sites could interact with vicinal diol ribose negatively charged structure ATP, respectively. However, P1 P4 boronic group site had poor selectivity detection. In contrast, P2, P3, P5, P6 exhibited better than P4. particular, P2 more advantages high sensitivity, selectivity, good time stability P6, which was ascribed its structure, linker 1 (1,4-bis(bromomethyl)benzene), recognition sites. Then, detect it low limit 3.62 μM. Moreover, showed utility monitoring mitochondrial level fluctuations.

Язык: Английский

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Polarity-Driven Fluorescence Monitoring of Lipid Droplet Dynamics in Dry Eye Disease

Analytical Chemistry, Год журнала: 2024, Номер 96(24), С. 9975 - 9983

Опубликована: Июнь 3, 2024

The emergence of lipid droplets (LDs) has been recognized as cellular markers ocular surface hyperosmosis, which is a fundamental mechanism driving dry eye disease (DED), while their dynamics during DED progression and therapy remains unlocked. For this purpose, an LD-specific fluorescent probe P1 presented in work that exhibits highly selective sensitive emission enhancement response to decreased ambient polarity (Δf) from 0.209 0.021. hydrophobic nature enables specific staining LDs, facilitating visualization changes within these structures. Utilizing P1, we observe decrease accompanied by increase the size number LDs hyperosmotic human corneal epithelial cells (HCECs). Furthermore, interplays between organelles such mitochondria Golgi apparatus are visualized, suggesting underlying pathogenesis DED. Notably, variations observed after inhibition ferroptosis or activation autophagy HCECs, implying great potential indicators for design efficacy evaluation drugs regarding targets. Finally, confirmed be overproduced tissues mice, application clinical drops effectively impedes changes. This detailed exploration underscores significant roles indicator deep insight into advancement therapy.

Язык: Английский

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Novel Ultrasensitive Fluorescent Probe for Bioimaging Carboxylesterase and Detecting Pesticide Residues in Foods

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(37), С. 20615 - 20621

Опубликована: Сен. 6, 2024

Pesticide residues pose a significant threat to food safety and human health, necessitating the development of novel detection tools. Pesticides can inhibit activity certain biological enzymes, so enzyme inhibition is one methods pesticide detection. In this study, we developed near-infrared fluorescent probe named

Язык: Английский

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In Situ Visualizing Carboxylesterase Activity in Type 2 Diabetes Mellitus Using an Activatable Endoplasmic Reticulum Targetable Proximity Labeling Far-Red Fluorescent Probe

Analytical Chemistry, Год журнала: 2024, Номер 96(26), С. 10724 - 10731

Опубликована: Июнь 19, 2024

Carboxylesterase (CE), an enzyme widely present in organisms, is involved various physiological and pathological processes. Changes the levels of CEs liver may predict presence type 2 diabetes mellitus (T2DM). Here, a novel dicyanoisophorone (DCI)-based proximity-labeled far-red fluorescent probe DCI2F-Ac with endoplasmic reticulum targeting was proposed for real-time monitoring imaging activity. featured very low cytotoxicity biotoxicity highly selective sensitive CEs. Compared traditional probes, covalently anchored directly to CEs, thus effectively reducing loss situ signals due diffusion. Through "on–off" fluorescence signal readout, able distinguish cell lines screen inhibitors. In terms (ER) stress, it found that thapsigargin (Tg) induced upregulation but not tunicamycin (Tm), which related calcium homeostasis ER. could efficiently detect downregulated livers T2DM, therapeutic efficacy metformin, acarbose, combination these two drugs assessed by tracking fluctuation levels. The results showed combining metformin acarbose restore near-normal best antidiabetic effect. Thus, provides great opportunity explore untapped potential metabolic disorders drug assessment.

Язык: Английский

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Fluorescent sensors for discriminative detection of multiple targets in pathological processes

Coordination Chemistry Reviews, Год журнала: 2023, Номер 489, С. 215203 - 215203

Опубликована: Май 9, 2023

Язык: Английский

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Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe

Chinese Chemical Letters, Год журнала: 2024, Номер 35(12), С. 110020 - 110020

Опубликована: Май 16, 2024

Язык: Английский

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