Royal Society of Chemistry eBooks,
Год журнала:
2024,
Номер
unknown, С. 271 - 292
Опубликована: Окт. 31, 2024
Proteins
play
a
crucial
role
in
shaping
cellular
processes,
requiring
accurate
recognition,
imaging,
and
dynamic
tracking
for
understanding
complex
biological
networks.
The
evolution
of
protein
labeling,
progressing
from
covalent
coupling
to
advanced
methodologies
like
click
chemistry,
genetically
encoded
tags,
proximity
has
provided
unprecedented
attributes.
Fluorescent
which
relies
heavily
on
diverse
technologies,
predominantly
employs
organic
dye-based
fluorophores
expanded
beyond
mere
tagging
proteins.
This
chapter
explores
the
significance
fluorogenic
probes
emphasizing
their
achieving
efficient,
high
resolution,
wash-free
imaging.
journey
into
mechanisms,
specificities,
advantages
reveals
as
essential
allies
precision
visualizing
functions.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(36), С. 14660 - 14668
Опубликована: Авг. 24, 2024
Bovine
serum
albumin
(BSA)
has
been
widely
used
in
biosensors
as
a
blocking
agent.
Herein,
conformist
BSA
was
first
exploited
an
ingenious
operator
to
enhance
the
photocurrent
response
of
(2Z,2′Z)-2,2′-(1,4-phenylene)bis(3-(4-(bis(4-methoxyphenyl)amino)phenyl)acrylonitrile)
(TPDCN)-based
photoelectrochemical
(PEC)
platform
via
manipulating
electron
transfer
process
detection
system.
Concretely,
presence
target
molecules
triggered
catalytic
hairpin
assembly
reaction
and
subsequently
powered
terminal
deoxynucleotidyl
transferase-mediated
signal
amplification
produce
AgNP@BSA-DNA
dendrimer
nanostructure.
After
being
treated
with
HNO3,
large
amount
could
be
released
from
When
they
were
transferred
TPDCN-based
PEC
platform,
biosensor
largely
enhanced
because
can
manipulate
electrons
TPDCN
well-matched
energy
level
form
new
track.
Meanwhile,
tryptophan
(Trp)
oxidized
quinone
Trp-O
under
photoirradiation,
which
facilitate
oxidation
ascorbate
generate
more
H+
promote
migration
photogenerated
electrons.
As
result,
proposed
exhibits
excellent
analytical
performance
for
miRNA-21
(as
model
target)
over
wide
linear
range
0.01
10,000
pM
limit
low
4.7
fM.
Overall,
this
strategy
provides
perspective
on
constructing
efficient
biosensors,
expands
potential
applications
bioanalysis
clinical
diagnosis.
ACS Sensors,
Год журнала:
2024,
Номер
9(6), С. 3170 - 3177
Опубликована: Июнь 11, 2024
Super-resolution
fluorescence
imaging
has
emerged
as
a
potent
tool
for
investigating
the
nanoscale
structure
and
function
of
plasma
membrane
(PM).
Nevertheless,
challenge
persists
in
achieving
super-resolution
PM
dynamics
due
to
limitations
probe
photostability
issues
with
cell
internalization
staining.
Herein,
we
report
assembly-mediated
buffering
fluorogenic
probes
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 30, 2024
Abstract
Super‐resolution
fluorescence
imaging
of
live
cells
increasingly
demands
fluorescent
probes
capable
multi‐color
and
long‐term
dynamic
imaging.
Understanding
the
mechanisms
probe‐target
recognition
is
essential
for
engineered
development
such
probes.
In
this
study,
it
discovered
that
molecular
lipid
solubility
parameter,
Clog
P
,
determines
staining
performance
dyes
on
droplets
(LDs).
Fluorescent
with
values
between
2.5
4
can
form
buffering
pools
outside
LDs,
replacing
photobleached
within
LDs
to
maintain
constant
intensity
in
thereby
enabling
super‐resolution
LDs.
Guided
by
four
different
colored
LD
spanning
visible
light
spectrum
have
been
developed.
Using
Structured
Illumination
Microscopy
(SIM),
role
dynamics
tracked
during
cellular
ferroptosis
secretion,
storage,
degradation
overexpressed
ACSL3
proteins.
It
found
serve
as
storage
sites
these
proteins
through
membrane
fusion,
further
degrade
via
interactions
organelles
like
lysosomes
or
lipophagy,
maintaining
homeostasis.
Chemical Communications,
Год журнала:
2024,
Номер
60(11), С. 1424 - 1427
Опубликована: Янв. 1, 2024
In
this
study,
we
have
uncovered
that
trifluoroethylamine-substituted
solvatochromic
fluorophores
maintain
consistently
high
and
stable
fluorescence
intensity
in
diverse
polar
environments,
including
highly
protic
solvents.
Chemical Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Azetidinyl
Malachite
Green
(Aze-MG)
enhances
the
brightness
and
photostability
of
MG-FAP
system
by
suppressing
TICT
effect,
enabling
stable
dynamic
super-resolution
imaging
through
a
buffering
strategy.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
In
recent
years,
small
molecular
fluorescent
probes
have
significantly
advanced
biosensing
and
cancer
therapy,
enabling
applications
such
as
target
detection,
cellular
imaging,
fluorescence-guided
surgery,
phototherapy.
Chemical Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
By
incorporating
hybrid
FRET
pairs
with
RFPs
as
energy
donors
and
TMSiR
the
acceptor,
intersystem
crossing
(ISC)
of
is
suppressed
via
FRET,
leading
to
enhanced
photostability
RFPs.
