A Siderophore Mimicking Gelation Component for Capturing and Self-Separation of Fe(III) from an Aqueous Solution of Mixture of Metal Ions DOI
Ritika Munjal,

Reena Kyarikwal,

Sayantan Sarkar

и другие.

Inorganic Chemistry, Год журнала: 2024, Номер 63(15), С. 7089 - 7103

Опубликована: Апрель 4, 2024

The carbohydrazide-based gelation component N2,N4,N6-(1,3,5-triazine-2,4,6-triyl)tris(benzene-1,3,5-tricarbohydrazide) (CBTC) was synthesized and characterized using various spectroscopic tools. CBTC trimesic acid (TMA) get self-assembled to form metallogel with Fe3+, specifically through noncovalent interactions in a DMSO H2O mixture. self-assembly shows remarkable specificity toward Fe(III) among different transition metal salts. It is pertinent point out that the binding for Fe3+ can also be found nature of siderophores, as they are mainly involved scavenging iron selectively from surroundings. DFT studies have been used investigate possible interaction between components metallogel. To determine selectivity iron, CBTC, along acid, interact other ions, including single system. TMA bind iron(III), which leads formation gets separated discrete layer, leaving ions solution. Therefore, together show iron-scavenging properties. This selective property explored FE-SEM, XPS, PXRD, IR, ICP-AES analysis. FE-SEM analysis flower-petal-like morphology resemblance CBTC-TMA-Fe obtained mixture salts established images XPS release achieved help ascorbic converts Fe2+. In biological systems, released similarly siderophores. first report where molecule capable self-separating aqueous presence ions.

Язык: Английский

Cyanochelin B: A siderophore produced by cyanobacteriumLeptolyngbyasp. NIES-3755 with photolytic properties that negate iron monopolization in the UV-light DOI Creative Commons
Berness Falcao,

Viviana Di Matteo,

Pavel Hrouzek

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Abstract Siderophores are low-molecular-weight compounds excreted by microorganisms to facilitate iron uptake in times of its unavailability. Microbes may produce siderophores monopolize and achieve competitive exclusion other strains. Alternatively, be exchanged for substrates mutualistic relationships. that employ β -hydroxy-aspartate ( -OH-Asp) chelation were shown undergo UV-mediated photolytic cleavage with simultaneous reduction Fe 3+ 2+ . Photolytic can mediate algal-bacterial mutualism, where the bacteria provide exchange dissolved organic carbon. We use an interdisciplinary strategy a complex characterization cyanochelin B, β-OH-Asp-containing siderophore produced filamentous cyanobacterium Leptolyngbya sp. NIES-3755. A combination nuclear magnetic resonance, high resolution mass spectrometry bioinformatic analyses complemented Marfey’s Murata’s methods yielded structure B configuration stereocenters. Cyanochelin B-iron complexes exposed UV- light photolyze within minutes (t 1/2 = 8.9 min; ∼3.5 uE UV-A) release reduced have co-cultured together Synechocystis PCC6803 as reporter strain lacking production. The cultivation setup was based on membrane-separated compartments accommodating individual strains employed alginate-embedded FeCl 3 simulate poorly accessible precipitated iron. Our results demonstrate absence UV-light favor Leptolyngbya. However, eliminates any monopolization makes it available competing organisms. Finally, we report isolation novel B-producing Phormidesmis from field material discuss phenomenon broader context. Importance Iron is essential micronutrient required all living organisms cofactor indispensable enzymes. Due specific properties mostly biologically unavailable. To uptake, microbes - bind mediators microbial interactions non-compatible or foster partners cheater full structural elucidation cyanobacterial contains β-OH-Asp. co-culture experiments show producer make depending presence UV light. Moreover, our data suggest benefits production not restricted symbiotic but rather whole irradiated community. Of known siderophores, 17.5% contain photoreactive β-OH-Asp function similar way.

Язык: Английский

Процитировано

0
In Silico Analysis of the Ga3+/Fe3+ Competition for Binding the Iron-Scavenging Siderophores of P. aeruginosa—Implementation of Three Gallium-Based Complexes in the “Trojan Horse” Antibacterial Strategy DOI Creative Commons
Nikoleta Kircheva, Stefan Dobrev, Vladislava Petkova

и другие.

Biomolecules, Год журнала: 2024, Номер 14(4), С. 487 - 487

Опубликована: Апрель 16, 2024

The emergence of multidrug-resistant (MDR) microorganisms combined with the ever-draining antibiotic pipeline poses a disturbing and immensely growing public health challenge that requires multidisciplinary approach application novel therapies aimed at unconventional targets and/or applying innovative drug formulations. Hence, bacterial iron acquisition systems Fe2+/3+-containing enzymes have been identified as plausible target great potential. intriguing “Trojan horse” deprives from essential iron. Recently, gallium’s potential in medicine an mimicry species has attracted vast attention. Different Ga3+ formulations exhibit diverse effects upon entering cell thus supposedly multiple targets. aim current study is to specifically distinguish characteristics significance regard initial gallium-based complex, allowing alien cation effectively compete native ferric ion for binding siderophores pyochelin pyoverdine secreted by bacterium P. aeruginosa. Therefore, three were taken into consideration: first-generation gallium nitrate, Ga(NO3)3, metabolized Ga3+-hydrated forms, second-generation maltolate (tris(3-hydroxy-2-methyl-4-pyronato)gallium), experimentally proven Ga carrier bloodstream—the protein transferrin. We employed reliable silico based on DFT computations order understand underlying biochemical processes govern Ga3+/Fe3+ rivalry two siderophores.

Язык: Английский

Процитировано

3
Implementation of Three Gallium-Based Complexes in the “Trojan Horse” Antibacterial Strategy against A. baumannii: A DFT Approach DOI
Nikoleta Kircheva, Stefan Dobrev, Valya Nikolova

и другие.

Inorganic Chemistry, Год журнала: 2024, Номер 63(33), С. 15409 - 15420

Опубликована: Авг. 8, 2024

Microorganisms of the ESKAPE group pose an enormous threat to human well-being, thus requiring a multidisciplinary approach for discovering novel drugs that are not only effective but utilize innovative mechanism action in order decrease fast developing resistance. A promising still hardly explored implementation "Trojan horse" antibacterial strategy has been recognized gallium, iron mimicry species with no known function exerting bacteriostatic/bactericidal effect against some representatives group. The study herewith focuses on bacterium A. baumannii and its siderophore acinetobactin two isomeric forms depending acidity medium. By applying powerful tools DFT approach, we aim delineate those physicochemical characteristics great importance potentiating gallium's ability compete native ferric cation binding such as pH, solvent exposure (dielectric constant environment), different metal/siderophore ratios, complex composition. Hence, provided results furnish explanation positive three Ga3+-based anti-infectives terms metal competition also shed light reported vitro vivo observations at molecular level regard baumannii.

Язык: Английский

Процитировано

2
Exploring plant symbiotic microbial dynamics in metal hyperaccumulators for phytoremediation DOI

Sarita Tiwari,

Abid Ullah, Yuxi Feng

и другие.

International Biodeterioration & Biodegradation, Год журнала: 2024, Номер 196, С. 105941 - 105941

Опубликована: Окт. 14, 2024

Язык: Английский

Процитировано

2
A new Ag(I) complex based on asymmetric azine-type ligand as a promising anticancer agent: Synthesis, X-ray structure, and DFT calculations DOI
Mezna Saleh Altowyan, Saied M. Soliman,

M. Abu-Youssef

и другие.

Journal of Molecular Structure, Год журнала: 2024, Номер unknown, С. 140908 - 140908

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1
A Siderophore Mimicking Gelation Component for Capturing and Self-Separation of Fe(III) from an Aqueous Solution of Mixture of Metal Ions DOI
Ritika Munjal,

Reena Kyarikwal,

Sayantan Sarkar

и другие.

Inorganic Chemistry, Год журнала: 2024, Номер 63(15), С. 7089 - 7103

Опубликована: Апрель 4, 2024

The carbohydrazide-based gelation component N2,N4,N6-(1,3,5-triazine-2,4,6-triyl)tris(benzene-1,3,5-tricarbohydrazide) (CBTC) was synthesized and characterized using various spectroscopic tools. CBTC trimesic acid (TMA) get self-assembled to form metallogel with Fe3+, specifically through noncovalent interactions in a DMSO H2O mixture. self-assembly shows remarkable specificity toward Fe(III) among different transition metal salts. It is pertinent point out that the binding for Fe3+ can also be found nature of siderophores, as they are mainly involved scavenging iron selectively from surroundings. DFT studies have been used investigate possible interaction between components metallogel. To determine selectivity iron, CBTC, along acid, interact other ions, including single system. TMA bind iron(III), which leads formation gets separated discrete layer, leaving ions solution. Therefore, together show iron-scavenging properties. This selective property explored FE-SEM, XPS, PXRD, IR, ICP-AES analysis. FE-SEM analysis flower-petal-like morphology resemblance CBTC-TMA-Fe obtained mixture salts established images XPS release achieved help ascorbic converts Fe2+. In biological systems, released similarly siderophores. first report where molecule capable self-separating aqueous presence ions.

Язык: Английский

Процитировано

0