Customized AIEgen-Based Molecular Signaling Tags Combined Microfluidic Chip for Point-of-Care Testing Viable E. coli O157:H7

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Pathogenic bacterial infections pose a significant threat to human life, health, and socioeconomic development, with those arising from Escherichia coli (E. coli) O157:H7 being particularly concerning. Herein, customized aggregation-induced emission luminogens (AIEgen)-based signaling tags (TPA-galactose) were combined microfluidic chip for the determination of E. O157:H7. TPA-galactose undergoes hydrolysis by β-galactosidase, resulting in formation highly fluorescent TPA–OH AIE characteristics. Phages covalently bound surface magnetic beads specifically capture lyse O157:H7, releasing endogenous fluorescence intensity facilitates The process achieves sensitivity 45 CFU/mL min, requiring no DNA extraction or amplification, utilizing minimal sample volume, enabling accurate one-stop quantification live This strategy enables rapid on-site environmental, food, clinical samples, significantly enhancing public health safety.

Язык: Английский

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Sample-in-answer-out centrifugal microfluidic chip reaction biosensor powered by Thermus thermophilus Argonaute (TtAgo) for rapid, highly sensitive and multiplexed molecular diagnostics of foodborne bacterial pathogens

Chemical Engineering Journal, Год журнала: 2024, Номер 495, С. 153434 - 153434

Опубликована: Июль 1, 2024

Язык: Английский

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Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Advanced Science, Год журнала: 2024, Номер 12(2)

Опубликована: Ноя. 22, 2024

Abstract Bacterial infection is a crucial factor resulting in public health issues worldwide, often triggering epidemics and even fatalities. The accurate, rapid, convenient detection of viable bacteria an effective method for reducing infections illness outbreaks. Here, unsupervised learning–assisted surface acoustic wave–interdigital transducer‐driven nano‐lens holography biosensing platform developed the ultrasensitive amplification‐free bacteria. monitoring device integrated with effect can achieve holographic imaging polystyrene microsphere probes ultra‐wide field view (∽28.28 mm 2 ), sensitivity limit as low 99 nm. A lightweight learning hologram processing algorithm considerably reduces training time computing hardware requirements, without requiring datasets manual labels. By combining phage–mediated bacterial DNA extraction enhanced CRISPR–Cas12a systems, this strategy successfully achieves Salmonella various real samples, demonstrating accuracy validated qPCR benchmark method. This approach has cost (∽$500) rapid (∽1 h) highly sensitive (∽38 CFU mL −1 allowing emerging powerful tool food safety inspection clinical diagnosis.

Язык: Английский

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Versatile sensing strategies based on emerging programmable prokaryotic Argonautes: From nucleic acid to non-nucleic acid targets

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118130 - 118130

Опубликована: Янв. 1, 2025

Язык: Английский

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Harnessing CRISPR technology for next-generation microphysiological systems

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер 185, С. 118164 - 118164

Опубликована: Янв. 31, 2025

Язык: Английский

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Argonaute protein powered biosensing for pathogenic biosafety

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 305, С. 141321 - 141321

Опубликована: Фев. 19, 2025

Язык: Английский

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Artificial Intelligence in Bacterial Diagnostics and Antimicrobial Susceptibility Testing: Current Advances and Future Prospects

Biosensors and Bioelectronics, Год журнала: 2025, Номер 280, С. 117399 - 117399

Опубликована: Март 19, 2025

Язык: Английский

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Advances in optical sensor visualization: Enabling rapid mycotoxin detection

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 105003 - 105003

Опубликована: Апрель 1, 2025

Язык: Английский

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Microfluidics in smart food safety

Advances in food and nutrition research, Год журнала: 2024, Номер unknown, С. 305 - 354

Опубликована: Янв. 1, 2024

Язык: Английский

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Programmable and ultra-efficient Argonaute protein-mediated nucleic acid tests: A review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 278, С. 134755 - 134755

Опубликована: Авг. 13, 2024

Язык: Английский

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