Versatile fluorescence biosensors based on CRISPR/Cas12a for determination of site-specific DNA methylation from blood and tissues DOI

Lihua Ding,

Shengnan Cao, Liping Bai

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 329, P. 125520 - 125520

Published: Dec. 2, 2024

Language: Английский

Customized AIEgen-Based Molecular Signaling Tags Combined Microfluidic Chip for Point-of-Care Testing Viable E. coli O157:H7 DOI
Feng Niu, Yiqi Li,

Yongkun Zhao

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Pathogenic bacterial infections pose a significant threat to human life, health, and socioeconomic development, with those arising from Escherichia coli (E. coli) O157:H7 being particularly concerning. Herein, customized aggregation-induced emission luminogens (AIEgen)-based signaling tags (TPA-galactose) were combined microfluidic chip for the determination of E. O157:H7. TPA-galactose undergoes hydrolysis by β-galactosidase, resulting in formation highly fluorescent TPA–OH AIE characteristics. Phages covalently bound surface magnetic beads specifically capture lyse O157:H7, releasing endogenous fluorescence intensity facilitates The process achieves sensitivity 45 CFU/mL min, requiring no DNA extraction or amplification, utilizing minimal sample volume, enabling accurate one-stop quantification live This strategy enables rapid on-site environmental, food, clinical samples, significantly enhancing public health safety.

Language: Английский

Citations

1
Sample-in-answer-out centrifugal microfluidic chip reaction biosensor powered by Thermus thermophilus Argonaute (TtAgo) for rapid, highly sensitive and multiplexed molecular diagnostics of foodborne bacterial pathogens DOI
Nan Wang, Qiang Zhang, Yajie Liu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 495, P. 153434 - 153434

Published: July 1, 2024

Language: Английский

Citations

7
Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria DOI Creative Commons
Yang Zhou, Junpeng Zhao, Junping Wen

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 12(2)

Published: Nov. 22, 2024

Abstract Bacterial infection is a crucial factor resulting in public health issues worldwide, often triggering epidemics and even fatalities. The accurate, rapid, convenient detection of viable bacteria an effective method for reducing infections illness outbreaks. Here, unsupervised learning–assisted surface acoustic wave–interdigital transducer‐driven nano‐lens holography biosensing platform developed the ultrasensitive amplification‐free bacteria. monitoring device integrated with effect can achieve holographic imaging polystyrene microsphere probes ultra‐wide field view (∽28.28 mm 2 ), sensitivity limit as low 99 nm. A lightweight learning hologram processing algorithm considerably reduces training time computing hardware requirements, without requiring datasets manual labels. By combining phage–mediated bacterial DNA extraction enhanced CRISPR–Cas12a systems, this strategy successfully achieves Salmonella various real samples, demonstrating accuracy validated qPCR benchmark method. This approach has cost (∽$500) rapid (∽1 h) highly sensitive (∽38 CFU mL −1 allowing emerging powerful tool food safety inspection clinical diagnosis.

Language: Английский

Citations

5
Versatile sensing strategies based on emerging programmable prokaryotic Argonautes: From nucleic acid to non-nucleic acid targets DOI
Xianfeng Lin,

Lixin Kang,

Jiaqi Feng

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118130 - 118130

Published: Jan. 1, 2025

Language: Английский

Citations

0
Harnessing CRISPR technology for next-generation microphysiological systems DOI Creative Commons

S. Song,

Hyun Wook Kang,

Minjin Kang

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 185, P. 118164 - 118164

Published: Jan. 31, 2025

Language: Английский

Citations

0
Argonaute protein powered biosensing for pathogenic biosafety DOI
Yaru Li, Lu Zhao, Long Ma

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141321 - 141321

Published: Feb. 19, 2025

Language: Английский

Citations

0
Artificial Intelligence in Bacterial Diagnostics and Antimicrobial Susceptibility Testing: Current Advances and Future Prospects DOI
Seungmin Lee, Jeong‐Soo Park, Ji Hye Hong

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 280, P. 117399 - 117399

Published: March 19, 2025

Language: Английский

Citations

0
Advances in optical sensor visualization: Enabling rapid mycotoxin detection DOI

Qingxin Chuai,

Xuecheng Zhu,

Ying An

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 105003 - 105003

Published: April 1, 2025

Language: Английский

Citations

0
Microfluidics in smart food safety DOI
Liyuan Gong, Yang Lin

Advances in food and nutrition research, Journal Year: 2024, Volume and Issue: unknown, P. 305 - 354

Published: Jan. 1, 2024

Language: Английский

Citations

3
Programmable and ultra-efficient Argonaute protein-mediated nucleic acid tests: A review DOI
Jinlong Jiao, Dandan Zeng,

Yafang Wu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134755 - 134755

Published: Aug. 13, 2024

Language: Английский

Citations

3