International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 13839 - 13855
Опубликована: Дек. 1, 2024
Successful regeneration of cranial defects necessitates the use porous bone fillers to facilitate cell proliferation and nutrient diffusion. Open microspheres, characterized by their high specific surface area osteo-inductive properties, offer an optimal microenvironment for ingrowth efficient ossification, potentially accelerating regeneration.
Язык: Английский
Bioactive Materials, Год журнала: 2024, Номер 40, С. 227 - 243
Опубликована: Июнь 14, 2024
Язык: Английский
Процитировано
9
Advanced Healthcare Materials, Год журнала: 2024, Номер unknown
Опубликована: Сен. 10, 2024
Abstract The healing of bone defects among diabetic patients presents a critical challenge due to the pathological microenvironment, characterized by hyperglycemia, excessive reactive oxygen species (ROS) production, and inflammation. Herein, multifunctional composite microspheres, termed GMAP are developed, using microfluidic technique incorporating Au@Pt nanoparticles (NPs) GelMA hydrogel modulate microenvironment for promoting regeneration. enables sustained release NPs, which function as bimetallic nanozymes with dual enzyme‐like activities involving glucose oxidase catalase. synergistic effect allows efficient consumption ROS elimination concurrently. Thus, effectively protects proliferation marrow mesenchymal stem cells (BMSCs) under adverse high‐glucose conditions. Furthermore, it also promotes osteogenic differentiation paracrine capabilities BMSCs, subsequently inhibits inflammation enhances angiogenesis. In vivo rats defect model, is demonstrated that microspheres significantly improve regeneration, verified micro‐computed tomography histological examinations. This study provides novel strategy regeneration modulating presenting promising approach addressing complex challenges associated in patients.
Язык: Английский
Процитировано
4
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101588 - 101588
Опубликована: Фев. 19, 2025
Язык: Английский
Процитировано
0
Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13
Опубликована: Фев. 28, 2025
Introduction Bone defect repair remains a major challenge in modern medicine. Although bone marrow mesenchymal stem cells (BMSCs) possess multilineage differentiation potential, traditional BMSC constructs are often limited clinical applications due to insufficient osteogenic efficiency and inadequate vascularization. Methods This study developed an innovative tissue engineering strategy by combining BMSCs with gelatin/polycaprolactone (GT/PCL) nanofiber membranes form cell sheets, which were then modified endothelial (ECs) on the surface. The sheets subsequently rolled into three-dimensional scaffolds systematically evaluate their potential underlying mechanisms. Resuilts Results showed that electrospun GT/PCL exhibited uniform fiber structure (diameter 200–500 nm), successfully mimicking microstructure of natural extracellular matrix. In vitro experiments demonstrated after 14 days culture, EC modification significantly enhanced compared unmodified controls, approximately 3-fold increase ALP expression (p < 0.05) 2.5-fold angiogenic factor VEGF 0.01). Subcutaneous implantation nude mice revealed superior formation capability EC-modified at both 4 8 weeks: micro-CT analysis density reaching 350 mg/cm 3 , surface area approaching 400 mm 2 volume fraction 20%, higher than control groups 0.0001). Immunohistochemical evaluation further confirmed more mature trabecular richer vascular networks groups. Discussion Mechanistic studies promoted regeneration through three key pathways: optimization local microenvironment for improved nutrient supply, activation intercellular synergistic signaling pathways, reconstruction physiological microenvironment. not only validates application value this composite but also provides important theoretical basis developing novel solutions.
Язык: Английский
Процитировано
0
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Апрель 16, 2025
Cartilage tissue engineering has made significant strides in clinical regenerative treatment. The success of cartilage regeneration critically depends on a favorable microenvironment by means ideal bioactive scaffolds. However, total meniscus replacement frequently entails harsh accompanying chronic inflammation and oxidative stress conditions after massive injury, which extremely hinders repair. Herein, "core-shell" codelivery nanocarrier is developed to synergistically regulate the cartilaginous immune (CIME) for replacement. In this study, mesoporous silica nanoparticles are used encapsulate an antioxidant anti-inflammatory drug, Emodin, core meanwhile modify growth differentiation factor (GDF) reversible disulfide bonds shell, together constructing system (Em@MSN-GDF). synergistic dual-drug release effectively reverses followed successful promotion fibrocartilage vivo. Subsequently, Em@MSN-GDF-loaded cartilage-specific matrix hydrogels combined with meniscus-shaped polycaprolactone framework construct mechanically reinforced living substitute. As result, rabbit experiments demonstrate that regulates microenvironment, thereby achieving regeneration. current therefore, offers nanotreatment strategy reverse
Язык: Английский
Процитировано
0
Accounts of Materials Research, Год журнала: 2024, Номер unknown
Опубликована: Сен. 22, 2024
Язык: Английский
Процитировано
3
Coordination Chemistry Reviews, Год журнала: 2024, Номер 527, С. 216400 - 216400
Опубликована: Дек. 16, 2024
Язык: Английский
Процитировано
2
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 11(1), С. 182 - 207
Опубликована: Дек. 12, 2024
The treatment of bone defects in diabetes mellitus (DM) patients remains a major challenge since the diabetic microenvironments significantly impede regeneration. Many abnormal factors including hyperglycemia, elevated oxidative stress, increased inflammation, imbalanced osteoimmune, and impaired vascular system microenvironment will result high rate impaired, delayed, or even nonhealing events tissue. Stimuli-responsive biomaterials that can respond to endogenous biochemical signals have emerged as effective therapeutic systems treat via combination microenvironmental regulation enhanced osteogenic capacity. Following natural healing processes, coupling angiogenesis osteogenesis by advanced bioresponsive drug delivery has proved be significant approach for promoting repair DM. In this Review, we systematically summarized mechanisms strategies DM-induced healing, outlined design systems, highlighted vascularization Accordingly, then overview recent advances developing facilitate vascularized regeneration remodeling modulating multiple regenerative cues. Furthermore, discuss development adaptable with unique features guiding DM-associated future.
Язык: Английский
Процитировано
1
Materials Today Bio, Год журнала: 2024, Номер 30, С. 101413 - 101413
Опубликована: Дек. 18, 2024
Язык: Английский
Процитировано
0
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 13839 - 13855
Опубликована: Дек. 1, 2024
Successful regeneration of cranial defects necessitates the use porous bone fillers to facilitate cell proliferation and nutrient diffusion. Open microspheres, characterized by their high specific surface area osteo-inductive properties, offer an optimal microenvironment for ingrowth efficient ossification, potentially accelerating regeneration.
Язык: Английский
Процитировано
0