Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158139 - 158139
Опубликована: Ноя. 1, 2024
Язык: Английский
Advanced Materials, Год журнала: 2024, Номер 36(34)
Опубликована: Июнь 11, 2024
The repair and functional reconstruction of bone defects resulting from severe trauma, surgical resection, degenerative disease, congenital malformation pose significant clinical challenges. Bone tissue engineering (BTE) holds immense potential in treating these defects, without incurring prevalent complications associated with conventional autologous or allogeneic grafts. 3D printing technology enables control over architectural structures at multiple length scales has been extensively employed to process biomimetic scaffolds for BTE. In contrast inert grafts, next-generation smart possess a remarkable ability mimic the dynamic nature native extracellular matrix (ECM), thereby facilitating regeneration. Additionally, they can generate tailored controllable therapeutic effects, such as antibacterial antitumor properties, response exogenous and/or endogenous stimuli. This review provides comprehensive assessment progress 3D-printed BTE applications. It begins an introduction physiology, followed by overview technologies utilized scaffolds. Notable advances various stimuli-responsive strategies, efficacy, applications are discussed. Finally, highlights existing challenges development implementation scaffolds, well emerging this field.
Язык: Английский
Процитировано
53
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
Regeneration of diabetic bone defects remains a formidable challenge due to the chronic hyperglycemic state, which triggers accumulation advanced glycation end products (AGEs) and reactive oxygen species (ROS). To address this issue, we have engineered bimetallic metal–organic framework-derived Mn@Co3O4@Pt nanoenzyme loaded with alendronate Mg2+ ions (termed MCPtA) regulate microenvironment recover osteogenesis/osteoclast homeostasis. Notably, Mn atom substitution in Co3O4 nanocrystalline structure could modulate electronic significantly improve SOD/CAT catalytic activity for ROS scavenging. By integration GOx-like Pt nanoparticles, MCPtA achieved effective multiple cascade performance that facilitated clearance glucose ROS. Furthermore, was encapsulated within glucose-responsive hydrogel cross-linked via borate ester bond, termed PAM, evaluate potential composite cranial defect repair rats. The vitro/vivo experiments as well RNA sequencing analysis demonstrated disrupt glucose-ROS-induced inflammation promoted osteogenesis angiogenesis, consequence, improving therapeutic effects regeneration. This study provided crucial insights into nanoenzyme-mediated microenvironmental regulation
Язык: Английский
Процитировано
3
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 289, С. 138633 - 138633
Опубликована: Дек. 13, 2024
Язык: Английский
Процитировано
9
Chemical Engineering Journal, Год журнала: 2024, Номер 490, С. 151792 - 151792
Опубликована: Апрель 29, 2024
Язык: Английский
Процитировано
8
Advanced Materials, Год журнала: 2024, Номер unknown
Опубликована: Окт. 22, 2024
Conventional bone tissue engineering materials struggle to reinstate physiological remodeling in a diabetic context, primarily due the compromised repolarization of proinflammatory macrophages anti-inflammatory macrophages. Here, leveraging single-cell RNA sequencing (scRNA-seq) technology, pivotal role nitric oxide (NO) and reactive oxygen species (ROS) is unveiled impeding macrophage during amidst diabetes. Guided by scRNA-seq analysis, we engineer multienzymatic hydrogel scaffold (MEBTHS) composed engineered methylpropenylated gelatin integrated with ruthenium nanozymes, possessing both Ru
Язык: Английский
Процитировано
7
Tissue Engineering Part B Reviews, Год журнала: 2025, Номер unknown
Опубликована: Янв. 6, 2025
Synthetic bone transplantation has emerged in recent years as a highly promising strategy to address the major clinical challenge of tissue defects. In this field, bioactive glasses (BGs) have been widely recognized viable alternative traditional substitutes due their unique advantages, including favorable biocompatibility, pronounced bioactivity, excellent biodegradability, and superior osseointegration properties. This article begins with comprehensive overview development success BGs engineering, then focuses on composite reinforcement systems biodegradable metals, calcium-phosphorus (Ca-P)-based bioceramics, medical polymers, respectively. Moreover, outlines some frequently used manufacturing methods for three-dimensional BG-based bioscaffolds highlights remarkable achievements these scaffolds field defect repair years. Lastly, based many potential challenges encountered preparation application BGs, brief outlook future directions is presented. review may help provide new ideas researchers develop ideal reconstruction functional recovery.
Язык: Английский
Процитировано
0
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101483 - 101483
Опубликована: Янв. 11, 2025
Injuries to the central nervous system (CNS) often lead persistent inflammation and limited regeneration. This study developed a clinically relevant injectable hydrogel derived from decellularized human peripheral nerves, with mechanical properties biomimicking native CNS tissue. Using modified Hudson method, sciatic nerves were decellularized, effectively removing immunogenic cellular debris while retaining extracellular matrix. Two delipidation solvents, dichloromethane: ethanol (2:1 v/v) n-hexane: isopropanol (3:1 v/v), evaluated, former achieving optimal lipid removal better digestion. The resulting solution was crosslinked genipin, forming an (iHPN) that gelled within 12 min at 37 °C exhibited stiffness of approximately 400 Pa. Human astrocytes, microglial cell clone 3 (HMC3), mouse RAW 264.7 macrophages cultured individually iHPN, lipopolysaccharide (LPS) added mimic following injury. Compared LPS-activated cells on tissue culture plates (TCP), astrocytes iHPN maintained quiescent state, as evidenced by reduced GFAP expression IL-1β secretion. HMC3 in displayed anti-inflammatory phenotype, shown increased CD206 decreased CD86/CD68 expression, along higher IL-4 lower TNF-α/IL-1β SH-SY5Y neuroblastoma viability improved neuronal differentiation compared TCP. brain neurons had TCP or collagen hydrogels. Overall, is novel has potential for minimally invasive applications, such carrier drug delivery and/or biomaterial support axonal growth.
Язык: Английский
Процитировано
0
Biomaterials Advances, Год журнала: 2025, Номер 169, С. 214199 - 214199
Опубликована: Янв. 23, 2025
Язык: Английский
Процитировано
0
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101533 - 101533
Опубликована: Янв. 29, 2025
Bone defects resulting from trauma or disease remain a significant challenge in clinical practice, often requiring prolonged treatment. Poly(ether-ketone-ketone) (PEKK) is commonly used implant material due to its excellent biocompatibility and mechanical properties, which are similar those of bone. However, biological inertness leads poor anti-inflammatory osteointegration significantly hindering the bone repair process. In this study, cryogel filled - PEKK/bioglass (BG) composite scaffold (SPBC) was prepared via 3D printing provide immunomodulatory integration performance. Compared with untreated PEKK, SPBC exhibited enhancements surface including higher hydrophilicity roughness. Additionally, enhanced adsorption fibronectin vitronectin on regulated maturation cytoskeleton adhesion plaques by increasing phosphorylation level FAK at Y397, thereby promoting cell spreading. Due release bioactive ions, can promote polarization RAW264.7 cells towards M2 secretion cytokines, while also enhancing proliferation differentiation rat mesenchymal stem (rMSCs) vitro. Furthermore, vivo results confirmed properties host tissue. summary, after modification filling, demonstrated abilities, presenting potential for application as an orthopedic scaffold.
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Март 8, 2025
Periodontitis is a chronic inflammatory disease caused by plaque. In order to remove pathogens and promote tissue repair, the following steps need be taken simultaneously: localizing diseased area, improving anaerobic microenvironment, as well addressing anti-inflammatory osteogenic needs. This study aims address these issues developing responsive near-infrared-IIb nanozyme system (DMUP), assembled from lanthanide-doped down-converted nanoparticles multi-enzymatically active nanozyme. DMUP binds bacterial membranes via targeting peptide ubiquicidin29-41 (UBI29-41). Upon responding it releases manganese (Mn) paeonol (Pae), localized infected areas fluorescent imaging in near-infrared IIb (NIR-IIb) region. particular, released Mn reacts with hydrogen peroxide microenvironment generate oxygen (O2) situ, thereby anoxic environment inhibit bacteria. On other hand, metal oxide nanozyme, scavenges reactive species (ROS) mimicking cascade process of superoxide dismutase catalase. The phenolic antioxidant Pae shifts macrophages pro-inflammatory (M1-type) (M2-type) through Akt/mTOR pathway. It can synergize regulate reducing inflammation, promoting genes expression, accelerating periodontal tissues regeneration.
Язык: Английский
Процитировано
0