Salinity tolerance in plants. Quantitative approach to ion transport starting from halophytes and stepping to genetic and protein engineering for manipulating ion fluxes DOI Creative Commons
Vadim Volkov

Frontiers in Plant Science, Год журнала: 2015, Номер 6

Опубликована: Окт. 27, 2015

Ion transport is the fundamental factor determining salinity tolerance in plants. The Review starts from differences ion between salt tolerant halophytes and salt-sensitive plants with an emphasis on of potassium sodium via plasma membranes. comparison provides introductory information for increasing tolerance. Effects stress properties membranes show huge opportunities manipulating fluxes. Further steps require knowledge about mechanisms individual genes proteins. Initially, describes methods to measure fluxes, independent set techniques ensures robust reliable basement quantitative approach. briefly summarises current data concerning Na+ K+ concentrations cells, refers primary thermodynamics gives special attention channels transporters. Simplified scheme a plant cell known systems at membrane tonoplast helps imagine complexity allows choose specific transporters modulating transport. enhanced by influence size wall Special given HKT, HAK, NHX SOS1 Comparison nonselective cation reveals potential importance balance two pathways detail several successful attempts overexpress or knockout changing Future perspectives are questioned more promising candidate altered expression. Potential direction modifying using single point mutations discussed questioned. An alternative approach synthetic biology create new regulation networks novel proteins desired transforming agricultural crops. had not been widely used earlier; it leads also theoretical pure scientific aspects protein chemistry, structure-function relations proteins, physiology homeostasis. Summarising, ways aimed required increase

Язык: Английский

All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins DOI
Daniel R. Hochbaum, Yongxin Zhao, Samouil L. Farhi

и другие.

Nature Methods, Год журнала: 2014, Номер 11(8), С. 825 - 833

Опубликована: Июнь 22, 2014

Язык: Английский

Процитировано

754
Genetically encoded indicators of neuronal activity DOI
Michael Z. Lin, Mark J. Schnitzer

Nature Neuroscience, Год журнала: 2016, Номер 19(9), С. 1142 - 1153

Опубликована: Авг. 26, 2016

Язык: Английский

Процитировано

620
The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins DOI
Erik A. Rodriguez, Robert E. Campbell, John Y. Lin

и другие.

Trends in Biochemical Sciences, Год журнала: 2016, Номер 42(2), С. 111 - 129

Опубликована: Ноя. 1, 2016

Язык: Английский

Процитировано

576
Genetic Dissection of Neural Circuits: A Decade of Progress DOI Creative Commons
Liqun Luo, Edward M. Callaway, Karel Svoboda

и другие.

Neuron, Год журнала: 2018, Номер 98(2), С. 256 - 281

Опубликована: Апрель 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Язык: Английский

Процитировано

445
High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor DOI
François St-Pierre, Jesse D. Marshall,

Ying Yang

и другие.

Nature Neuroscience, Год журнала: 2014, Номер 17(6), С. 884 - 889

Опубликована: Апрель 22, 2014

Язык: Английский

Процитировано

407
Astrocytes Control Circadian Timekeeping in the Suprachiasmatic Nucleus via Glutamatergic Signaling DOI Creative Commons
Marco Brancaccio, Andrew P. Patton, Johanna E. Chesham

и другие.

Neuron, Год журнала: 2017, Номер 93(6), С. 1420 - 1435.e5

Опубликована: Март 1, 2017

The suprachiasmatic nucleus (SCN) of the hypothalamus orchestrates daily rhythms physiology and behavior in mammals. Its circadian (∼24 hr) oscillations gene expression electrical activity are generated intrinsically can persist indefinitely temporal isolation. This robust resilient timekeeping is generally regarded as a product intrinsic connectivity its neurons. Here we show that neurons constitute only one "half" SCN clock, metabolically active during daytime. In contrast, astrocytes nighttime, when they suppress by regulating extracellular glutamate levels. glutamatergic gliotransmission sensed dorsal via specific pre-synaptic NMDA receptor assemblies containing NR2C subunits. Remarkably, somatic genetic re-programming intracellular clocks was capable remodeling behavioral adult mice. Thus, circuit-level arises from interdependent mutually supportive astrocytic-neuronal signaling.

Язык: Английский

Процитировано

390
Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo DOI Creative Commons
Helen H. Yang, François St-Pierre, Xulu Sun

и другие.

Cell, Год журнала: 2016, Номер 166(1), С. 245 - 257

Опубликована: Июнь 1, 2016

Язык: Английский

Процитировано

263
Visualizing Whole-Brain Activity and Development at the Single-Cell Level Using Light-Sheet Microscopy DOI Creative Commons
Philipp Keller, Misha B. Ahrens

Neuron, Год журнала: 2015, Номер 85(3), С. 462 - 483

Опубликована: Фев. 1, 2015

Язык: Английский

Процитировано

234
Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila DOI Creative Commons

Zachary A. Knecht,

Ana F. Silbering, Lina Ni

и другие.

eLife, Год журнала: 2016, Номер 5

Опубликована: Сен. 22, 2016

Ionotropic Receptors (IRs) are a large subfamily of variant ionotropic glutamate receptors present across Protostomia. While these most extensively studied for their roles in chemosensory detection, recent work has implicated two family members, IR21a and IR25a, thermosensation Drosophila. Here we characterize one the evolutionarily deeply conserved receptors, IR93a, show that it is co-expressed functions with IR25a to mediate physiological behavioral responses cool temperatures. IR93a also distinct receptor, IR40a, discrete population sensory neurons sacculus, multi-chambered pocket within antenna. We demonstrate this combination required neuronal dry air discrimination humidity differences. Our results identify as common component molecularly cellularly IR pathways important hygrosensation insects.

Язык: Английский

Процитировано

222
Whole-central nervous system functional imaging in larval Drosophila DOI Creative Commons
William C. Lemon, Stefan R. Pulver,

Burkhard Höckendorf

и другие.

Nature Communications, Год журнала: 2015, Номер 6(1)

Опубликована: Авг. 11, 2015

Abstract Understanding how the brain works in tight concert with rest of central nervous system (CNS) hinges upon knowledge coordinated activity patterns across whole CNS. We present a method for measuring an entire, non-transparent CNS high spatiotemporal resolution. combine light-sheet microscope capable simultaneous multi-view imaging at volumetric speeds 25-fold faster than state-of-the-art, whole-CNS assay isolated Drosophila larval and computational framework analysing multi-view, calcium data. image both ventral nerve cord, covering entire 2 or 5 Hz two- one-photon excitation, respectively. By mapping network during fictive behaviours quantitatively comparing high-resolution maps individuals, we predict functional connections between regions reveal neurons that identify type temporal state motor programs executed cord.

Язык: Английский

Процитировано

215